E. Coli

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3.1. Materials: 3.1.1. Sample: 3.MATERIAL AND METHODS A total of 295 samples obtained from livers, spleen, heart of freshly died broiler, one day old chicks and layers in Dakahlia province. A total of 53 children, male and female urine samples were obtained from human private laboratories. 3.1.2. Media used: 3.1.2.1. Selective enrichment broth: Selenite F broth (Edward and Ewing, 1972) Bacteriological peptone 5.0 g/l Lactose 4.0 g/l Sodium phosphate 10.0 g/l 3.1.2.2. Selective culture media: 3.1.2.2.1. Nutrient Agar (Oxoid) Lab-lemco powder 1.0 g/l Yeast extract 2.0 g/l Peptone 2.0 g/l Sodium chloride 5.0 g/l Agar pH 7.4 15.0 g/l 3.1.2.2.2. Blood Agar (Oxoid) Lab-lemco, powder 10.0 g/l Peptone 10.0 g/l 29
Sodium chloride 5.0 g/l Agar pH 7.3 15.0 g/l 3.1.2.2.3. MacConkey's Agar (Oxoid): Peptone 20.0 g/l Lactose 10.0 g/l Bile salts 5.0 g/l Sodium chloride 5.0 g/l Neutral red 0.075 g/l Agar pH 7.4 12.0 g/l 3.1.2.2.4. Salmonella Shigella Agar (Oxoid): Lab-lemco powder 5.0 g/l Peptone 5.0 g/l Lactose 10.0 g/l Bile salts 5.5 g/l Sodium citrate 10.0 g/l Sodium thiosulphate 8.5 g/l Ferric citrate 1.0 g/l Brilliant green 0.00033 g/l Neutral red 0.025 g/l Agar pH 7.3 12.0 g/l 3.1.2.2.5. Levins Eosin Methylene Blue (Oxoid) Peptone 10.0 g/l 30
Page 1 and 2: A STUDY ON THE USE OF ANTIBIOTICS I
Page 3 and 4: List of contents Page 1-Introductio
Page 5 and 6: Tables Page Table(1)Biochemical rea
Page 7 and 8: Acknowledgements First of all thank
Page 9: 2 Introduction certain serogroups,
Page 13 and 14: Sodium citrate, tribasic 2.0 g/l So
Page 15 and 16: 3.1.4.Solutions and indicators in b
Page 17 and 18: Polyvalent 4: O6 , O27 , O78 , O148
Page 19 and 20: serological identification accordin
Page 21 and 22: 6- Urease test The test determine t
Page 23 and 24: 3. The plate was swabbed in differe
Page 25 and 26: 2.REVIEW OF LITERATURE 2.1. Inciden
Page 27 and 28: Review of Literature suggested that
Page 29 and 30: Review of Literature K5 (37.5%), 15
Page 31 and 32: Review of Literature within Ireland
Page 33 and 34: Review of Literature salmonella spe
Page 35 and 36: Review of Literature Sasipreeyajan
Page 37 and 38: Review of Literature Moreno et al.
Page 39 and 40: Review of Literature Mohamad (1996)
Page 41 and 42: Review of Literature pencillin (68.
Page 43 and 44: Review of Literature El-Ghamdi et a
Page 45 and 46: Review of Literature serious ever l
Page 47 and 48: Review of Literature the time from
Page 49 and 50: Review of Literature isolates from
Page 51 and 52: 4.Results A total of 295 chicken sa
Page 53 and 54: Table (4): Incidence of chicken Ent
Page 55 and 56: Abolila 11 10/11(90.9%) 10/10(100%)
Page 57 and 58: fermentation (Lactose, Maltose, Man
Page 59 and 60: isolates were salmonella, pseudomon
Page 61 and 62:
Fig.(3)Triple sugar iron agar(TSI),
Page 63 and 64:
Fig.(4).Citrate negative E.coli iso
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Fig.(6)An isolate was sensitive to
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Farm Mansoura1 Omda1 Asher3 Asher4
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Gentamycin, Colistin, Erythromycin,
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Trimethoprim), Colistin, Erythromyc
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(Sulpamethox-azole/ Trimethoprim),
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Table(10)Sensitivity pattern for is
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R = Resistant S = Sensitive CTX30 =
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Table(12 )Sensitivity Pattern of is
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Doxycyclin, Erythromycin, were effe
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Fig.(8) Sensitivity percentages of
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Discussion Concerning bacteriologic
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al., (1997) reported that 10% of E.
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1/2(50%) Salmonella broiler isolate
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12/14(85.71%) E.coli isolates from
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streptomycin, meanwhile higher perc
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Two out of eleven(18.18%) E.coli is
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and Nguyen (1995) who reported mode
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Summary Bacterial diseases have lon
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epresented 105/138(76.08%) and 33/1
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100%, 100%, 63.63%, 63.63%, 63.63%,
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3-Results of antibiogram on biotype
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a pediatric cohort in aperiurbab ar
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Bozorgmehri O., Fard M.H. and Gilan
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importance in some poultry farms. 7
Page 114 and 115:
Fisher M.E., Trampel D. W. and Grif
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Hui, A.K. and Das, R., (2000): Prev
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Kikuvi, G.M.; Ole-Mapenay, I.M.; Mi
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Machado J. and Bernardo F. (1990):
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Oxoid manual (1998): The oxoid manu
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and O 157 Serotypes from piglets an
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Van Donkersgoed J., Manninen K., Po
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ﻦﻴﺘﺴﻟﻮآ , ﻦﻴﺳﺎ
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- She received her primary educatio
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E. Coli

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