E. Coli
E. Coli
E. Coli
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3.1. Materials:<br />
3.1.1. Sample:<br />
3.MATERIAL AND METHODS<br />
A total of 295 samples obtained from livers, spleen, heart of<br />
freshly died broiler, one day old chicks and layers in Dakahlia<br />
province.<br />
A total of 53 children, male and female urine samples were<br />
obtained from human private laboratories.<br />
3.1.2. Media used:<br />
3.1.2.1. Selective enrichment broth:<br />
Selenite F broth (Edward and Ewing, 1972)<br />
Bacteriological peptone 5.0 g/l<br />
Lactose 4.0 g/l<br />
Sodium phosphate 10.0 g/l<br />
3.1.2.2. Selective culture media:<br />
3.1.2.2.1. Nutrient Agar (Oxoid)<br />
Lab-lemco powder 1.0 g/l<br />
Yeast extract 2.0 g/l<br />
Peptone 2.0 g/l<br />
Sodium chloride 5.0 g/l<br />
Agar<br />
pH 7.4<br />
15.0 g/l<br />
3.1.2.2.2. Blood Agar (Oxoid)<br />
Lab-lemco, powder 10.0 g/l<br />
Peptone 10.0 g/l<br />
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