Investigation of retention mechanisms in HILIC chromatography ...
Investigation of retention mechanisms in HILIC chromatography ...
Investigation of retention mechanisms in HILIC chromatography ...
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log k'<br />
1.20<br />
1.00<br />
0.80<br />
0.60<br />
0.40<br />
0.20<br />
Response <strong>of</strong> Ephedr<strong>in</strong>e Retention on Buffer<br />
Concentration on Three <strong>HILIC</strong> Phases<br />
y = -0.6525x + 1.2104<br />
R 2 = 0.9984<br />
y = -0.8187x + 1.1259<br />
R 2 = 1<br />
y = -0.2199x + 0.2455<br />
R 2 = 0.9934<br />
0.00<br />
0.00 0.20 0.40 0.60 0.80 1.00 1.20<br />
log buffer concentration (mM)<br />
OH5 <strong>HILIC</strong> F5 L<strong>in</strong>ear (<strong>HILIC</strong>) L<strong>in</strong>ear (F5) L<strong>in</strong>ear (OH5)<br />
<strong>HILIC</strong> OH5 column at 10 and 2 mM ammonium<br />
acetate<br />
10 mM - 3<br />
A1849_032_A005 Sm (Mn, 2x3) 1: Scan ES+<br />
3.05<br />
166<br />
2.91e8<br />
%<br />
1<br />
Time<br />
1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00<br />
2 mM - 3<br />
A1849_032_A025 Sm (Mn, 2x3) 1: Scan ES+<br />
6.49<br />
168<br />
7.84e7<br />
%<br />
1<br />
10 mM<br />
2 mM<br />
1, 2<br />
1, 2<br />
3<br />
Time<br />
1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00<br />
More polar synephr<strong>in</strong>e elutes last<br />
= partition<br />
Little response <strong>of</strong> <strong>retention</strong> on<br />
buffer conc.<br />
3<br />
1. Ephedr<strong>in</strong>e<br />
2. Pseudoephedr<strong>in</strong>e<br />
3. Synephr<strong>in</strong>e<br />
24/10/2012<br />
8
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