Investigation of retention mechanisms in HILIC chromatography ...
Investigation of retention mechanisms in HILIC chromatography ...
Investigation of retention mechanisms in HILIC chromatography ...
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
log k'<br />
1.20<br />
1.00<br />
0.80<br />
0.60<br />
0.40<br />
0.20<br />
Response <strong>of</strong> Ephedr<strong>in</strong>e Retention on Buffer<br />
Concentration on Three <strong>HILIC</strong> Phases<br />
y = -0.6525x + 1.2104<br />
R 2 = 0.9984<br />
y = -0.8187x + 1.1259<br />
R 2 = 1<br />
y = -0.2199x + 0.2455<br />
R 2 = 0.9934<br />
0.00<br />
0.00 0.20 0.40 0.60 0.80 1.00 1.20<br />
log buffer concentration (mM)<br />
OH5 <strong>HILIC</strong> F5 L<strong>in</strong>ear (<strong>HILIC</strong>) L<strong>in</strong>ear (F5) L<strong>in</strong>ear (OH5)<br />
<strong>HILIC</strong> OH5 column at 10 and 2 mM ammonium<br />
acetate<br />
10 mM - 3<br />
A1849_032_A005 Sm (Mn, 2x3) 1: Scan ES+<br />
3.05<br />
166<br />
2.91e8<br />
%<br />
1<br />
Time<br />
1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00<br />
2 mM - 3<br />
A1849_032_A025 Sm (Mn, 2x3) 1: Scan ES+<br />
6.49<br />
168<br />
7.84e7<br />
%<br />
1<br />
10 mM<br />
2 mM<br />
1, 2<br />
1, 2<br />
3<br />
Time<br />
1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00<br />
More polar synephr<strong>in</strong>e elutes last<br />
= partition<br />
Little response <strong>of</strong> <strong>retention</strong> on<br />
buffer conc.<br />
3<br />
1. Ephedr<strong>in</strong>e<br />
2. Pseudoephedr<strong>in</strong>e<br />
3. Synephr<strong>in</strong>e<br />
24/10/2012<br />
8