Investigation of retention mechanisms in HILIC chromatography ...
Investigation of retention mechanisms in HILIC chromatography ...
Investigation of retention mechanisms in HILIC chromatography ...
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<strong>Investigation</strong> <strong>of</strong> Retention Mechanisms on<br />
Different <strong>HILIC</strong> Phases<br />
• <strong>in</strong>teraction differences for three different <strong>HILIC</strong> stationary phases: PFPP (F5), bare<br />
silica (<strong>HILIC</strong>) and a new pentalol phase (OH5)<br />
• Us<strong>in</strong>g ephedr<strong>in</strong>e as a probe molecule, <strong>retention</strong> as a function <strong>of</strong> buffer<br />
concentration was collected and <strong>in</strong>terpreted.<br />
• Related compounds and dom<strong>in</strong>ant <strong>in</strong>teractions prevalent us<strong>in</strong>g each phase is<br />
studied<br />
Selected Probes<br />
HO<br />
C<br />
H 3<br />
C<br />
H 3<br />
C<br />
H 3<br />
C<br />
H 3<br />
NH<br />
NH<br />
OH<br />
OH<br />
HN<br />
CH 3<br />
OH<br />
ACD/Labs, PhysChemProp, v. 12<br />
Structure pKa(MB) LogD(8.0) LogP MW name<br />
9.38 -0.37 1.08 165.23 pseudoephedr<strong>in</strong>e<br />
9.38 -0.37 1.08 165.23 ephedr<strong>in</strong>e<br />
9.37 -1.35 0.13 167.21 synephr<strong>in</strong>e<br />
24/10/2012<br />
6
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