Investigation of retention mechanisms in HILIC chromatography ...
Investigation of retention mechanisms in HILIC chromatography ...
Investigation of retention mechanisms in HILIC chromatography ...
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Proposed Model for Different <strong>HILIC</strong> Stationary Phases<br />
OH5<br />
Aqueous Layer<br />
Aqueous Layer<br />
- - - - - - -<br />
-<br />
Conclusions<br />
Aqueous-Organic Mobile Phase<br />
Silica<br />
Polar Stationary Phase<br />
• Columns described <strong>in</strong> this presentation<br />
• Pentalol (OH5)– dom<strong>in</strong>ated by partition<br />
• Bare silica (<strong>HILIC</strong>)– both partition and IEX<br />
• Pentafluorophenylpropyl – ma<strong>in</strong>ly IEX<br />
• In order to develop robust and reliable methods us<strong>in</strong>g <strong>HILIC</strong><br />
<strong>chromatography</strong> thefollow<strong>in</strong>g factors should be considered<br />
• pH changes <strong>in</strong> high % organics<br />
• pKa <strong>of</strong> analytes <strong>in</strong> high % organics<br />
• Mobile phase modifiers concentrations<br />
F5<br />
24/10/2012<br />
10
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