SIEVE User Guide
SIEVE User Guide
SIEVE User Guide
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2. Make changes to the parameters as needed. Click Run.<br />
2 Setting up a Procedure<br />
Identifying Proteins and Peptides<br />
If you selected Show advanced progress info in the Submit Analysis view, a DOS<br />
window displays the progress of the procedure.<br />
At the end of the SEQUEST database search, <strong>SIEVE</strong> opens the Spotfire application.<br />
3. To see a consolidated summary of <strong>SIEVE</strong> results, click the Table View tab in the Spotfire<br />
main view. This table includes columns displaying the pValue and intensity ratio for each<br />
frame, as well as the SEQUEST search results, if applicable. <strong>SIEVE</strong> provides the results of<br />
the differential analysis in a table view so that you can check the protein or peptide<br />
columns and select a frame with useful protein or peptide information.<br />
For information about the various Spotfire views shown as tabs at the bottom of the<br />
screen or the sliding scale parameter settings, see Appendix C, “Using Spotfire to View<br />
Analysis Results.”<br />
Figure 12. Table view in Spotfire post-SEQUEST search<br />
Thermo Scientific <strong>SIEVE</strong> <strong>User</strong> <strong>Guide</strong> 21
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