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SIEVE User Guide

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2 Setting up a Procedure<br />

Setting Parameters<br />

Setting Parameters<br />

To set parameters<br />

1. After selecting files for the analysis, click Next to display the Set Parameters dialog box.<br />

<strong>SIEVE</strong> populates each parameter in an edit box with recommended default values based<br />

on the instrument used to acquire the first raw file listed in the Control group. You can<br />

modify these values from the original default.<br />

2. Change parameters as needed.<br />

Figure 7. Set Parameters dialog box<br />

<strong>SIEVE</strong> uses these parameters to define a frame to determine the MS intensity threshold<br />

and to define the limits of the experiment.<br />

12 <strong>SIEVE</strong> <strong>User</strong> <strong>Guide</strong> Thermo Scientific

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