SIEVE User Guide

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Preface Safety and Special Notices Contacting Us There are several ways to contact Thermo Fisher Scientific. To contact Technical Support Phone 800-685-9535 Fax 561-688-8736 E-mail TechSupport.C+MS@thermofisher.com Knowledge base www.thermokb.com Find software updates and utilities to download at www.mssupport.thermo.com. To order products or to get product updates Phone 800-532-4752 Fax 561-688-8731 Web site www.thermo.com/finnigan To suggest changes to documentation or to Help • Fill out a reader survey online at www.thermo.com/lcms-techpubs. • Send an e-mail message to the Technical Publications Editor at techpubs.finnigan-lcms@thermofisher.com. x SIEVE User Guide Thermo Scientific
Overview to SIEVE SIEVE software performs semi-quantitative differential analyses of sample populations by comparing spectral information from LC/MS analyses of control (healthy) and disease (treatment) samples. Any changes between the two sample sets indicate a differential protein expression. Elements from raw files that show significant statistical differences are sent for a SEQUEST ® database search to determine peptide and protein identification. The following sections provide a high-level overview of SIEVE: • Understanding SIEVE • About the SIEVE Procedure Thermo Scientific SIEVE User Guide 1 1
Page 1 and 2: XCALI-97186 Revision B July 2007 Fo
Page 3 and 4: Contents Thermo Scientific SIEVE Us
Page 5 and 6: Preface SIEVE User Guide Viewing th
Page 7 and 8: Getting a License Getting a New Lic
Page 9: Installing a New License Code Once
Page 13 and 14: About the SIEVE Procedure 1 Overvie
Page 15 and 16: Setting up a Procedure The followin
Page 17 and 18: 2. Click Get Started to display exp
Page 19 and 20: 2 Setting up a Procedure Starting a
Page 21 and 22: 10. Click Add to add the files to t
Page 23 and 24: Table 1. Set Parameters dialog box
Page 25 and 26: Tips for Improving the Process Anal
Page 27 and 28: Spotfire displays a screen similar
Page 29 and 30: 2 Setting up a Procedure Framing th
Page 31 and 32: 2. Make changes to the parameters a
Page 33 and 34: Viewing a Saved Experiment 2 Settin
Page 35: 2 Setting up a Procedure Viewing a
Page 38 and 39: A Understanding the ChromAlign Proc
Page 46 and 47: B Understanding the Framing Process
Page 49 and 50: Using Spotfire to View Analysis Res
Page 51 and 52: Spotfire Range Sliders Frame Detail
Page 53 and 54: Protein ID View C Using Spotfire to
Page 55: Table 4. Parameter definitions for
Page 58: Index: P P parameters framing 37 se

SIEVE User Guide

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