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3 Investigation into the habitat of Romulea sabulosa and Romulea
Contents
monadelpha: Soil sampling and analysis 92
3.1 INTRODUCTION 92
3.2 MATERIALS AND METHODS 93
3.3 RESULTS 94
4.4 DISCUSSION 97
4.5 SUMMARY 97
4 Germination physiology 98
4.1 INTRODUCTION 98
4.2 MATERIALS AND METHODS 98
4.2.1 Viability tests 99
4.2.2 Water content and imbibition rate 100
4.2.3 Scanning electron microscopy 100
4.2.4 Ex vitro germination experiments 100
4.2.5 In vitro germination experiments 102
4.2.6 Statistical analysis 102
4.3 RESULTS 103
4.3.1 Viability tests 103
4.3.2 Water content and imbibition rate 103
4.3.3 Scanning electron microscopy 105
4.3.4 Ex vitro germination experiments 108
4.3.5 In vitro germination experiments 110
4.4 DISCUSSION 111
4.5 SUMMARY 114
5 In vitro culture initiation and multiplication 115
5.1 INTRODUCTION 115
5.2 MATERIALS AND METHODS 116
5.2.1 Explants from seedlings 116
5.2.2 Explants from embryos 117
5.2.3 Explant comparison 120
5.2.4 Shoot multiplication 120
5.2.5 Statistical analysis 121
5.3 RESULTS 121
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