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KCE reports vol. 6A Prenatale zorg 98
What methods should be used for screening for Chlamydia trachomatis in pregnancy?
Study Ref Population Intervention Outcomes Results Comments Study
Fitzgerald
et al.,
1998
Stary,
2001
4
[372]
4
[364]
Adults Culture
Enzyme
immunoassay
Serology
Adults Culture
Direct fluorescent
antibody assays
Enzyme
immunoassays
RNA-DNA
hybridisation
Nucleic acid
amplification
Sensitivities
and
specificities of
various tests
Sensitivities
and
specificities of
various tests
Chlamydia culture
Sensitivity 75% to 85% at best, and
may be low as 55%, only appropriate
for invasive samples, and labour
intensive
Enzyme immunoassay
Sensitivity 75% to 80% compared
with culture
Suitable for large number of samples,
requires invasive samples and high
specificity only if positive results are
confirmed
Serology of no value in diagnosis of acute
chlamydial infection
Cell culture sensitivity range 40% to 85%,
only appropriate for invasive samples
Direct fluorescent antibody assays
sensitivity range 50% to 90%, suitable for
invasive and noninvasive samples, but timeconsuming
and therefore unsuitable for
large numbers
Enzyme immunoassays sensitivity range
20% to 85%, suitable for large number of
samples, requires invasive samples and high
specificity only if positive results are
confirmed
Work carried out in
collaboration with Royal
College of Physicians
Research Unit and
Members of Central Audit
Group in Genitourinary
Medicine
Single author, no guideline
methodology given
EL
type
GL 4
GL 4