2007, Piran, Slovenia

Cold physiology
cold receptors decreased after menthol application in group A. The weakly sensitive to
menthol receptors, depolarizing under its effect, proved to respond more markedly to cold
exposure. This may be a reason why the response to cold was enhanced in-group C after
menthol application.
DISCUSSION
Taken together, the results demonstrated that menthol, which along with cold activates the
specific thermosensitive ion channels, changes the number of functioning cold receptors in
forearm skin, but is without effect on the warm receptor number. The disclosed group variants
in menthol response evidence for individual differences in skin receptor sensitivity to the
effect of menthol and cold. The results suggest that, based on the response variant to menthol
(decrease, increase or no change in the number of functioning cold receptors 5 min after
menthol application), the features of response to cold may be predicted.
REFERENCES
Kozyreva T.V. Adaptive changes in temperature sensitivity in humans under the conditions
of cold, heat and prolonged exercise. Human Physiology. 2006. 32, 721-725.
McKemy D. D., Neuhausser W.M., Julius D. Identification of a cold receptor reveals a
general role for TRP channels in thermosensation. Nature . 2002. 416, 52-58.
Jordt S.E, McKemy D.D, Julius D. Lessons from peppers and peppermint: the molecular logic
of thermosensation. . Curr. Opin. Neurobiol. 2003. (4), 487-492.
Reid G., Flonta M. Ion channels activated by cold and menthol in cultured rat dorsal root
ganglion neurons. Neurosci. Letter. 2002. 324, 164-168.
Viana F, de la Pena E, Belmonte C. Specificity of cold thermotransduction is determined by
differential ionic channel expression. Nat. Neurosci. 2002. 5, 189-190.
Wasner G., J. Schattschneider, A. Binder, R. Baron. Topical menthol – human model for cold
pain by activation and sensitization of nociceptors. Brain. 2004. 127, 1159-1171
Green BG. The sensory effects of l-menthol on human skin. Somatosens Mot Res. 1992. 9:
235-244.
Andersson D.A, Chase H.W, Bevan S. TRPM8 activation by menthol, icilin, and cold is
differentially modulated by intracellular pH. J. Neurosci. 2004. 24, 5364-5369.
Nealen M.L., Gold M.S., Thut P.D., Caterina M.J. TRPM8 mRNA Is Expressed in a Subset of
Cold-Responsive Trigeminal Neurons From Rat. J. Neurophysiol. 2003. 90, 515-520.
Tsuzuki K, Xing H, Ling J, Gu J.G. Menthol-induced Ca2+ release from presynaptic Ca2+
stores potentiates sensory synaptic transmission. J. Neurosci. 2004. 24, 762-771.
McKemy D. How cold is it? TRPM8 and TRPA1 in the molecular logic of cold. Molecular
Pain. 2005. 1-16.
Okazawa M., Inoue W., Hori A., Hosokawa H., Matsumura K., Kobayashi S. Noxious heat
receptors present in cold-sensory cells in rats. Neurosci. Lett. 2004. 359, 33-36.
Schaffer K., Braun H.A., Isenberg C. Effect of menthol on cold receptor activity. J. Gen.
Physiol. 1986. 88, 757-776.
Schaffer K., Braun H.A. Modulation of cutaneous cold receptor function by electrolytes,
hormones and thermal adaptation. Physiol. Res. 1992. 41: 71-75.
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