Analytical and Forensic Toxicology
Analytical and Forensic Toxicology Analytical and Forensic Toxicology
Blood Ethanol Testing • The law = whole blood • The lab = serum / plasma • [serum] = [plasma] • ETOH does not enter RBCs well • [serum]/[blood] ~ 1.15 • The lab measures higher than the law • [Breath ethanol] mmol/L X 2100 = [Blood ethanol] mmol/L
1. Wide turns 2. Straddling / driving on lane marker 3. Nearly striking object or another vehicle 4. Weaving / swerving 5. Going too slow (> 10 MPH below speed limit) The Clues 6. Stopping inappropriately / without cause 7. Following too closely 8. Erratic braking 9. Driving into opposing / crossing traffic 10.Slow response to traffic signals *NHTSA Top 10 in descending order of probability of intoxication
- Page 45 and 46: Gas Chromatography • Expensive
- Page 47 and 48: Mass Spectrometry • Can serve as
- Page 49 and 50: MS Limits • Cannot separate enant
- Page 51 and 52: GC/MS • Expensive equipment • M
- Page 53 and 54: Hair Analysis • Hair grows ~1 cm/
- Page 55 and 56: • Amphetamines • Cannabinoids
- Page 57 and 58: • • • • • Tetrahydrocanna
- Page 59 and 60: • Dronabinol • Efavirenz • NS
- Page 61 and 62: “Poppy Seed” Defense 6-MAM
- Page 63 and 64: Methadone False Positives • Queti
- Page 65 and 66: • Benzodiazepines Metabolized to
- Page 67 and 68: Nasal Inhalers • Can contain l-me
- Page 69 and 70: Federal Cutoffs (ng/mL) Substance S
- Page 71 and 72: Specimen Validity • Appearance
- Page 73 and 74: In Vitro Adulteration “Add after
- Page 75 and 76: Others • Acids and bases (i.e. le
- Page 77 and 78: Questions?
- Page 79 and 80: Butrylcholinesterase • Malnutriti
- Page 81 and 82: Anion Gap Reliability • Hypercalc
- Page 83 and 84: • • • Forensics Aid medical /
- Page 85 and 86: Medical Review Officer • • Lice
- Page 87 and 88: Post Mortem Toxicology
- Page 89 and 90: Specimens • Blood • Reported as
- Page 91 and 92: Other sources
- Page 93 and 94: Legal Ethanol
- Page 95: The Barman’s Paradox • Legally
- Page 99 and 100: What’s in a Drink? • 10 oz beer
- Page 101 and 102: • Specific gravity 0.8 g/ml • V
- Page 103 and 104: • • • • • • “Breathan
- Page 105: Photo References 1. http://www.offt
Blood Ethanol Testing<br />
• The law = whole blood<br />
• The lab = serum / plasma<br />
• [serum] = [plasma]<br />
• ETOH does not enter RBCs well<br />
• [serum]/[blood] ~ 1.15<br />
• The lab measures higher than the law<br />
• [Breath ethanol] mmol/L X 2100 = [Blood ethanol] mmol/L
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