Analytical and Forensic Toxicology
Analytical and Forensic Toxicology Analytical and Forensic Toxicology
High-Performance Liquid Chromatography • Stationary phase packed in a column • Mobile phase pumped through under pressure • Allows better separation in less time • Identification by retention time in a column • Also use ultraviolet spectroscopy • Measuring light absorbance allows the amount of the xenobiotic to be determined
Reverse phase chromatography • Non-polar stationary phase and hydrophilic mobile phase • Polar out first, non-polar retained • Hydrophobic stationary • Hydrophilic mobile phase • Most common HPLC technique • TLC can be done this way too
- Page 1 and 2: Analytical and Forensic Evan S. Sch
- Page 3 and 4: Content • Core Content of Medical
- Page 5 and 6: • Waived • Moderate complexity
- Page 7 and 8: Magic Answer Box? • Old methodolo
- Page 9 and 10: Testing “You’re fired, Jack. Th
- Page 11 and 12: Assay Methods • Chromatography
- Page 13 and 14: • Salicylates • • • Spot Te
- Page 15 and 16: Co-oximetry • Spectrophotometry u
- Page 17 and 18: Spectrochemical • Produce light a
- Page 19 and 20: Immunoassays • Created due to nee
- Page 21: Non-competitive “Sandwich” assa
- Page 29 and 30: Competitive From Goldfranks 9th edi
- Page 31 and 32: Immunoassays • Old - radio immuno
- Page 33 and 34: Magnetic Microparticle Goldfranks 9
- Page 35 and 36: Rf = Rf migration distance of subst
- Page 37 and 38: TLC Goldfranks 9th edition
- Page 39: TLC Drawbacks • Multiple steps
- Page 43 and 44: HPLC
- Page 45 and 46: Gas Chromatography • Expensive
- Page 47 and 48: Mass Spectrometry • Can serve as
- Page 49 and 50: MS Limits • Cannot separate enant
- Page 51 and 52: GC/MS • Expensive equipment • M
- Page 53 and 54: Hair Analysis • Hair grows ~1 cm/
- Page 55 and 56: • Amphetamines • Cannabinoids
- Page 57 and 58: • • • • • Tetrahydrocanna
- Page 59 and 60: • Dronabinol • Efavirenz • NS
- Page 61 and 62: “Poppy Seed” Defense 6-MAM
- Page 63 and 64: Methadone False Positives • Queti
- Page 65 and 66: • Benzodiazepines Metabolized to
- Page 67 and 68: Nasal Inhalers • Can contain l-me
- Page 69 and 70: Federal Cutoffs (ng/mL) Substance S
- Page 71 and 72: Specimen Validity • Appearance
- Page 73 and 74: In Vitro Adulteration “Add after
- Page 75 and 76: Others • Acids and bases (i.e. le
- Page 77 and 78: Questions?
- Page 79 and 80: Butrylcholinesterase • Malnutriti
- Page 81 and 82: Anion Gap Reliability • Hypercalc
- Page 83 and 84: • • • Forensics Aid medical /
- Page 85 and 86: Medical Review Officer • • Lice
- Page 87 and 88: Post Mortem Toxicology
- Page 89 and 90: Specimens • Blood • Reported as
Reverse phase<br />
chromatography<br />
• Non-polar stationary phase <strong>and</strong><br />
hydrophilic mobile phase<br />
• Polar out first, non-polar retained<br />
• Hydrophobic stationary<br />
• Hydrophilic mobile phase<br />
• Most common HPLC technique<br />
• TLC can be done this way too
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