Analytical and Forensic Toxicology
Analytical and Forensic Toxicology Analytical and Forensic Toxicology
Chromatography Separation and Detection •Encompasses several related techniques where analyte specificity is achieved by physical separation •Partition analytes between a stationary and mobile phase •Stationary: very fine particles aranged in a thin layer •Mobile (moving): phase flows thru spaces between particles •After separation--need a detection phase •However, can have provisional identification based on their characteristic velocities, distance traveled, or time to traverse the chromatography column •Not specific
Rf = Rf migration distance of substance migration distance of solvent front • Retention time • Time required to traverse the column • Retardation (planar) or retention (column) • Separation based on polarity, affinity, solubility, etc. • Standard for each analyte
- Page 1 and 2: Analytical and Forensic Evan S. Sch
- Page 3 and 4: Content • Core Content of Medical
- Page 5 and 6: • Waived • Moderate complexity
- Page 7 and 8: Magic Answer Box? • Old methodolo
- Page 9 and 10: Testing “You’re fired, Jack. Th
- Page 11 and 12: Assay Methods • Chromatography
- Page 13 and 14: • Salicylates • • • Spot Te
- Page 15 and 16: Co-oximetry • Spectrophotometry u
- Page 17 and 18: Spectrochemical • Produce light a
- Page 19 and 20: Immunoassays • Created due to nee
- Page 21: Non-competitive “Sandwich” assa
- Page 29 and 30: Competitive From Goldfranks 9th edi
- Page 31 and 32: Immunoassays • Old - radio immuno
- Page 33: Magnetic Microparticle Goldfranks 9
- Page 37 and 38: TLC Goldfranks 9th edition
- Page 39 and 40: TLC Drawbacks • Multiple steps
- Page 41 and 42: Reverse phase chromatography • No
- Page 43 and 44: HPLC
- Page 45 and 46: Gas Chromatography • Expensive
- Page 47 and 48: Mass Spectrometry • Can serve as
- Page 49 and 50: MS Limits • Cannot separate enant
- Page 51 and 52: GC/MS • Expensive equipment • M
- Page 53 and 54: Hair Analysis • Hair grows ~1 cm/
- Page 55 and 56: • Amphetamines • Cannabinoids
- Page 57 and 58: • • • • • Tetrahydrocanna
- Page 59 and 60: • Dronabinol • Efavirenz • NS
- Page 61 and 62: “Poppy Seed” Defense 6-MAM
- Page 63 and 64: Methadone False Positives • Queti
- Page 65 and 66: • Benzodiazepines Metabolized to
- Page 67 and 68: Nasal Inhalers • Can contain l-me
- Page 69 and 70: Federal Cutoffs (ng/mL) Substance S
- Page 71 and 72: Specimen Validity • Appearance
- Page 73 and 74: In Vitro Adulteration “Add after
- Page 75 and 76: Others • Acids and bases (i.e. le
- Page 77 and 78: Questions?
- Page 79 and 80: Butrylcholinesterase • Malnutriti
- Page 81 and 82: Anion Gap Reliability • Hypercalc
- Page 83 and 84: • • • Forensics Aid medical /
Chromatography<br />
Separation <strong>and</strong> Detection<br />
•Encompasses several related techniques where analyte specificity<br />
is achieved by physical separation<br />
•Partition analytes between a stationary <strong>and</strong> mobile phase<br />
•Stationary: very fine particles aranged in a thin layer<br />
•Mobile (moving): phase flows thru spaces between particles<br />
•After separation--need a detection phase<br />
•However, can have provisional identification based on their<br />
characteristic velocities, distance traveled, or time to traverse<br />
the chromatography column<br />
•Not specific
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