BOTANY Higher Secondary Second Year - Textbooks Online
BOTANY Higher Secondary Second Year - Textbooks Online
BOTANY Higher Secondary Second Year - Textbooks Online
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decarboxylation and dehydrogenation. This reaction is catalyzed by pyruvic<br />
dehydrogenase and two molecules of NAD + are reduced to NADH 2 .<br />
During this reaction two molecules of CO 2 are released. Oxidative<br />
decarboxylation of pyruvic acid occurs only under aerobic condition. Under<br />
anaerobic conditions, the pyruvic acid is reduced either to lactic acid or<br />
ethyl alcohol depending on the nature of the organism.<br />
5.3.3 Krebs cycle<br />
In 1937, Sir Hans Adolf Krebs described the catalytic role of pyruvic<br />
acid for the production of energy in the cell. The series of cyclic reactions<br />
involved in converting pyruvic acid to carbondioxide and water in<br />
mitochondria is called Krebs cycle. It is also known as citric acid cycle or<br />
tricarboxylic acid cycle – TCA cycle.<br />
1. In the first reaction of citric acid cycle, one molecule of acetyl<br />
Co~A combines with oxaloacetic acid to form citric acid. This reaction is<br />
catalyzed by citric acid synthetase. Citric acid contains three carboxylic<br />
acid groups.<br />
2. Citric acid is dehydrated to form cis-aconitic acid in the presence<br />
of aconitase<br />
3. The same enzyme aconitase catalyzes the formation of isocitric<br />
acid from cis-aconitic acid by the addition of a molecule of water. Citric<br />
acid, cis-aconitic acid and isocitric acid contain three carboxylic acid groups.<br />
4. The isocitric acid is oxidatively decarboxylated to α- ketoglutaric<br />
acid. This reaction is catalyzed by isocitric dehydrogenase. During this<br />
reaction, one NADH is formed.<br />
2<br />
5. The α- ketoglutaric acid is oxidatively decarboxylated to form<br />
succinyl Co~A. This reaction is catalyzed by α-ketoglutaric<br />
dehydrogenase. The energy released during this reaction is conserved in<br />
NADH . 2<br />
6. The succinyl Co~A is hydrolysed to succinic acid in the presence<br />
of succinyl Co-A synthetase. In this reaction, ADP is phosphorylated to<br />
ATP. This is called substrate level phosphorylation.<br />
7. The succinic acid is oxidized to form fumaric acid by succinic<br />
dehydrogenase. Here, FAD + is reduced to FADH 2 .<br />
8. The fumaric acid is converted to malic acid by the addition of a<br />
molecule of water. This reaction is catalyzed by fumarase.<br />
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