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4.4. Protoplast fusion Protoplasts are cells without a cell wall but bound by a plasma membrane. The isolated protoplasts are totipotent. Because of this unique property, plant protoplasts play a vital role in the field of genetic engineering. Protoplast technology includes the isolation, culture and fusion of higher plant protoplasts leading to the production of entire plants. You will be studying the method of isolation and fusion of protoplast in this chapter. A hybrid produced by fusion of somatic cells of two varieties (or) species is called somatic hybrid. This process of producing somatic hybrids is known as somatic hybridization. The first step in somatic hybridization is the isolation of protoplast. Isolation of protoplast Protoplast can be isolated from a variety of plant tissues using either mechanical (or) enzymatic methods. Mechanical method In this method, cells are kept in a suitable plasmolyticum (protoplast shrink away from cell wall in a plasmolysed cell) and cut with a fine knife, so that protoplasts are released from cells through the opening of the cell wall. This method gives poor yield of protoplast and it is being rarely used. Enzymatic method Leaves from a 10 week old plant are sterilized with 70 per cent alcohol and then treating them with 2 per cent solution of sodium hypochlorite for 20 to 30 minutes. The leaves are then washed with sterile water and subsequent procedures are done under aseptic conditions (using laminar air flow chamber). The lower epidermis of the leaf is peeled off and the leaf is cut into small fragments. From the peeled leaf segments, the protoplasts are isolated. For isolation of protoplast, peeled leaf segments are placed with their lower surface downwards in a petridishes containing the enzyme mixture, which consists of 0.5 per cent macerozyme, 2 per cent cellulase in 13 per cent sorbitol or mannitol at pH 5.4. Finally the protoplasts are released and are kept in the isotonic solution. 162
Strain A Strain B Isolation of protoplasts Explant A Explant B Cell wall removal by cellulase Protoplast fusion Fig.4.5 Protoplasmic fusion 163 Callus Regenerated plants
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BOTANY Higher Secondary Second Year
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Preface The most important and cruc
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1. Taxonomy PRACTICALS (30 periods)
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1. TAXONOMY OF ANGIOSPERMS Taxonomy
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compared to perianth with two whorl
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a total variety or species. To conc
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well known system of classification
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Self Evaluation I . Choose and writ
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Series (iii) Calyciflorae It includ
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Class III Monocotyledonae Seeds of
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5. How many families were described
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Flower Bracteate or ebracteate, bra
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A twig Sepal Epicalyx Calyx A petal
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Self evaluation I . Choose and writ
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Inflorescence Usually a raceme eg.
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Calyx Ovary Gynoecium Bracteole Flo
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5. Fibre plants Fibres obtained fro
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1.3.3. RUBIACEAE - the coffee famil
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Stem Leaf Aerial, erect, branched,
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1. Beverage plants ECONOMIC IMPORTA
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1.3.4. ASTERACEAE - the sunflower f
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Habit Root Stem Leaf Botanical desc
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Fruit Seed A cypsela. Non-endosperm
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Flower Bracteate (eg. Petunia hybri
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Stamen Epipetalous stamens T.S. of
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(eg. Jatropha curcas). In xerophyti
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Perianth Tepals 5, arranged in sing
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1. Food plants ECONOMIC IMPORTANCE
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MONOCOT FAMILY 1.3.7 MUSACEAE - the
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Habit Root Stem Botanical descripti
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Androecium Stamens 6, in two whorls
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1.3.8. ARECACEAE - the palm family
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Habit Botanical description of Coco
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Gynoecium Absent but pistillode is
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II. Answer the following questions
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Classification of meristem Based on
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oval, spherical, rectangular, cylin
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Fibres Fibre cells are dead cells.
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Tracheid conducting elements in ang
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a lining layer of cytoplasm. This i
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2. Epidermis protects the underlyin
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made up of barrel shaped parenchyma
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2.2. Anatomy of monocot and dicot r
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The endodermal cells, which are opp
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Cortex Cortex consists of only pare
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Ground plan A sector enlarged Fig.
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Ground plan A sector enlarged Fig.
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Xylem Xylem consists of xylem fibre
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2.4. Anatomy of a dicot and monocot
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Vascular tissues Vascular tissues a
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1. Formation of interfascicular cam
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Formation of periderm As secondary
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Each annual ring refers to one year
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8. What are complementary cells? 9.
Page 117 and 118: chromosome has one centromere and t
Page 119 and 120: Chromosomal puff Dark band inter ba
Page 121 and 122: The genome size of an individual is
Page 123 and 124: ound pollen (bbll). All the F1 hybr
Page 125 and 126: points called chiasmata. Crossing o
Page 127 and 128: 3.4. Recombination of chromosome Th
Page 129 and 130: Point or gene mutation Point mutati
Page 131 and 132: II. Answer the following questions
Page 133 and 134: 127 Fig. 3.6 Chromosomal aberration
Page 135 and 136: Autopolyploidy Ploidy - flow chart
Page 137 and 138: Self evaluation I . Choose and writ
Page 139 and 140: cells was injected into the mouse,
Page 141 and 142: Replication fork Super coil DNA pol
Page 143 and 144: 3.8. Structure of RNA and its types
Page 145 and 146: Role of RNA in protein synthesis Th
Page 147 and 148: The transfer of message present in
Page 149 and 150: IV. Answer the following questions
Page 151 and 152: to join DNA fragments. Thus the res
Page 153 and 154: Ti plasmid T-DNA Foreign DNA Bacter
Page 155 and 156: Restriction enzyme and ligase do no
Page 157 and 158: 4.2 Transgenic plants Introduction
Page 159 and 160: introducing Bt toxin gene in cotton
Page 161 and 162: 2. The number of transgenic plants
Page 163 and 164: Pot plant Explant Inoculation Surfa
Page 165 and 166: . Embryogenesis Formation of embryo
Page 167: II. Answer the following questions
Page 171 and 172: The term ‘single cell protein’
Page 173 and 174: 8. What is PEG? 9. How do you remov
Page 175 and 176: Many enzymes consists of a protein
Page 177 and 178: 5.1.3. Theories explaining the mech
Page 179 and 180: II. Answer the following questions
Page 181 and 182: 5.2.1. Significance of photosynthes
Page 183 and 184: The reactions of photosynthesis can
Page 185 and 186: 179 Fig. 5.8. Non-cyclic photophosp
Page 187 and 188: Difference between cyclic and noncy
Page 189 and 190: power NADPH 2 . So, two molecules o
Page 191 and 192: Fig.5.11 Hatch-Slack pathway of CO
Page 193 and 194: Difference between C 3 and C 4 phot
Page 195 and 196: Glycine molecules enter into mitoch
Page 197 and 198: Among leaf factors, such as leaf ag
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Page 201 and 202: When an insect is attracted by the
Page 203 and 204: 11. The gas evolved during photosyn
Page 205 and 206: 40. Explain the test tube and funne
Page 207 and 208: 201 Fig. 5.17 Overall scheme of res
Page 209 and 210: Fig. 5.18 Process of Glycolysis 203
Page 211 and 212: decarboxylation and dehydrogenation
Page 213 and 214: 9. The malic acid is oxidized to ox
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Respiratory quotient for anaerobic
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5. Fructose 1,6-bisphosphate is cle
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32. Write short notes on anaerobic
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this phase is called lag phase. It
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Physiological effects of auxin Auxi
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endosperm of coconut. Cytokinin occ
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4. Bakanae disease in paddy is caus
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5.5. Photoperiodism and vernalizati
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c. sunflower d. maize 4. Which of t
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1. To develop more food crops 2. To
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Clonal selection Crops like sugarca
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The haploid individual plant will h
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methods new improved crops are obta
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6.2. Crop diseases and their contro
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Pathogen The mycelium of Cercospora
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3. They also bring about considerab
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It is hoped that one day geneticall
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2. The genetic resources of the dev
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This will affect millions of uneduc
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additives. It depends upon crop rot
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COMMONLY AVAILABLE MEDICINAL PLANTS
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urinary infections, tuberculosis, m
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Groundnut oil is used to a limited
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