BOTANY Higher Secondary Second Year - Textbooks Online

Pot plant
Explant
Inoculation
Surface
sterilization
Medium
Induction of callus
Medium
Callus
Plantlets
Regeneration
of shoot and root
Pot plants
Transferred to field
(Hardening)
Fig. 4.4 Techniques of
tissue culture
157
1960s by Prof. P. Maheshwari and
Prof. S. Narayanaswamy at the
Department of Botany, University
of Delhi in India. Consequently,
media and culture techniques for a
variety of plant materials became
known, which are now extensively
utilised in all areas of plant
improvement programmes.
Basic techniques of plant tissue
culture
1. Culture vessels
The culture vessels used for
plant tissue studies includes
Erylenmayer flask (conical flask),
petri plates and culture tubes (25 x
150mm).
2. Culture medium
The important media used for
all purpose experiment are
Murashige and Skoog medium
(MS medium), Gamborg medium
(B5 medium), White medium
(W medium) and Nitsch medium.
The culture medium is closed with
cotton plug/ or aluminium foil
sheet. The pH of the medium is
adjusted to 5.8 (acidic range).
3. Sterilization
Sterilization is the technique
employed to get rid of the microbes
such as bacteria and fungi in the
culture medium and plant tissues.
So, it is important to sterilize the
culture medium and plant tissue.