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Transgenic microbes The genetically engineered micro-organisms are being used for the commercial production of some non-microbial products such as insulin, interferon, human growth hormone and viral vaccines. Use of genetically engineered bacterial strain In 1979, for the first time Anand Mohan Chakrabarty, an Indian born American scientist developed a strain of Pseudomonas putida that contained a hybrid plasmid derived by combining parts of CAM and OCT. (CAM and OCT are the plasmids which contain the genes responsible for the decomposition of the hydrocarbons like camphor and octane respectively present in the oil.) This strain could grew rapidly on crude oil because it was capable of metabolizing hydrocarbons more efficiently. The bacterial strain called the superbug was produced on a large scale in laboratory, mixed with straw and dried. When the straw was spread over oil slicks, the straw soaked up the oil and bacteria broke up the oil into non-polluting and harmless products. In this way, pollution of land and water due to the oil slicks can be remedied and the phenomenon is called bioremediation. It is defined as the use of living microorganisms to degrade environmental pollutants or prevent pollution. The contaminated sites are restored and future pollution is prevented. Benefits from release of genetically modified microorganisms into the environment. Protection of environment - Bioremediation of polluted environment. Microorganisms producing enzymes for food industry. Microorganisms with improved efficiency of fermentation. Improved microorganisms for milk industry. Microorganisms as live attenuated vaccines for health care. Increasing efficiency of plant nutrition, pest control (safe biopesticides), protection of plants from climatic stress and protection of plants from tumour formation and disease. Self evaluation I . Choose and write the correct options. 1. One of the following process is employed to introduce a foreign gene into a cell a. electrolysis b. electroporation c. plasmid d. ligation 154
2. The number of transgenic plants available to-day are approximately a. six b. two c. twelve d. fifty 3. A toxic protein called delta endotoxin is insecticidal and it is produced by a. Escherichia coli b. Streptomyces griseus c. Bacillus thuringiensis d. Bacillus lactii 4. Pseudomonas putida is a engineered bacterium that can a. produce a hormone b. produce a antibiotic c. digest crude oil slick d. pollute the soil II. Answer the following questions in two or three sentences. 5. Define biopesticides. 6. Define transgenic plants. 7. What is the importance of Agrobacterium tumefaciencs? 8. What is gene gun method of delivery of DNA? 9. Give the binomials of atleast two dicot transgenic plants. III. Answer the following questions in about 100 words. 10. What is the role of transgenic plants in food industry? 11. Give a brief account of herbicide resistance in transgenic plants. 12. How are foreign genes introduced into the plants? IV . Answer the following questions in about 200 words. 13. What is the role of Bt toxin in crop protection against pest? Explain the action of biopesticide. 14. Write an essay on transgenic plants. 155
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BOTANY Higher Secondary Second Year
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Preface The most important and cruc
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1. Taxonomy PRACTICALS (30 periods)
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1. TAXONOMY OF ANGIOSPERMS Taxonomy
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compared to perianth with two whorl
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a total variety or species. To conc
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well known system of classification
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Self Evaluation I . Choose and writ
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Series (iii) Calyciflorae It includ
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Class III Monocotyledonae Seeds of
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5. How many families were described
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Flower Bracteate or ebracteate, bra
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A twig Sepal Epicalyx Calyx A petal
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Self evaluation I . Choose and writ
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Inflorescence Usually a raceme eg.
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Calyx Ovary Gynoecium Bracteole Flo
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5. Fibre plants Fibres obtained fro
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1.3.3. RUBIACEAE - the coffee famil
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Stem Leaf Aerial, erect, branched,
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1. Beverage plants ECONOMIC IMPORTA
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1.3.4. ASTERACEAE - the sunflower f
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Habit Root Stem Leaf Botanical desc
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Fruit Seed A cypsela. Non-endosperm
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Flower Bracteate (eg. Petunia hybri
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Stamen Epipetalous stamens T.S. of
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(eg. Jatropha curcas). In xerophyti
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Perianth Tepals 5, arranged in sing
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1. Food plants ECONOMIC IMPORTANCE
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MONOCOT FAMILY 1.3.7 MUSACEAE - the
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Habit Root Stem Botanical descripti
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Androecium Stamens 6, in two whorls
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1.3.8. ARECACEAE - the palm family
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Habit Botanical description of Coco
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Gynoecium Absent but pistillode is
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II. Answer the following questions
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Classification of meristem Based on
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oval, spherical, rectangular, cylin
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Fibres Fibre cells are dead cells.
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Tracheid conducting elements in ang
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a lining layer of cytoplasm. This i
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2. Epidermis protects the underlyin
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made up of barrel shaped parenchyma
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2.2. Anatomy of monocot and dicot r
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The endodermal cells, which are opp
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Cortex Cortex consists of only pare
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Ground plan A sector enlarged Fig.
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Ground plan A sector enlarged Fig.
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Xylem Xylem consists of xylem fibre
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2.4. Anatomy of a dicot and monocot
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Vascular tissues Vascular tissues a
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Page 109 and 110: 1. Formation of interfascicular cam
Page 111 and 112: Formation of periderm As secondary
Page 113 and 114: Each annual ring refers to one year
Page 115 and 116: 8. What are complementary cells? 9.
Page 117 and 118: chromosome has one centromere and t
Page 119 and 120: Chromosomal puff Dark band inter ba
Page 121 and 122: The genome size of an individual is
Page 123 and 124: ound pollen (bbll). All the F1 hybr
Page 125 and 126: points called chiasmata. Crossing o
Page 127 and 128: 3.4. Recombination of chromosome Th
Page 129 and 130: Point or gene mutation Point mutati
Page 131 and 132: II. Answer the following questions
Page 133 and 134: 127 Fig. 3.6 Chromosomal aberration
Page 135 and 136: Autopolyploidy Ploidy - flow chart
Page 137 and 138: Self evaluation I . Choose and writ
Page 139 and 140: cells was injected into the mouse,
Page 141 and 142: Replication fork Super coil DNA pol
Page 143 and 144: 3.8. Structure of RNA and its types
Page 145 and 146: Role of RNA in protein synthesis Th
Page 147 and 148: The transfer of message present in
Page 149 and 150: IV. Answer the following questions
Page 151 and 152: to join DNA fragments. Thus the res
Page 153 and 154: Ti plasmid T-DNA Foreign DNA Bacter
Page 155 and 156: Restriction enzyme and ligase do no
Page 157 and 158: 4.2 Transgenic plants Introduction
Page 159: introducing Bt toxin gene in cotton
Page 163 and 164: Pot plant Explant Inoculation Surfa
Page 165 and 166: . Embryogenesis Formation of embryo
Page 169 and 170: Strain A Strain B Isolation of prot
Page 171 and 172: The term ‘single cell protein’
Page 173 and 174: 8. What is PEG? 9. How do you remov
Page 175 and 176: Many enzymes consists of a protein
Page 177 and 178: 5.1.3. Theories explaining the mech
Page 181 and 182: 5.2.1. Significance of photosynthes
Page 183 and 184: The reactions of photosynthesis can
Page 185 and 186: 179 Fig. 5.8. Non-cyclic photophosp
Page 187 and 188: Difference between cyclic and noncy
Page 189 and 190: power NADPH 2 . So, two molecules o
Page 191 and 192: Fig.5.11 Hatch-Slack pathway of CO
Page 193 and 194: Difference between C 3 and C 4 phot
Page 195 and 196: Glycine molecules enter into mitoch
Page 197 and 198: Among leaf factors, such as leaf ag
Page 199 and 200: Epiphyte eg. Vanda Aerial root Part
Page 201 and 202: When an insect is attracted by the
Page 203 and 204: 11. The gas evolved during photosyn
Page 205 and 206: 40. Explain the test tube and funne
Page 207 and 208: 201 Fig. 5.17 Overall scheme of res
Page 209 and 210: Fig. 5.18 Process of Glycolysis 203
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decarboxylation and dehydrogenation
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9. The malic acid is oxidized to ox
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is fixed in the vertical position w
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Nonoxidative phase In this phase, v
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Respiratory quotient for anaerobic
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5. Fructose 1,6-bisphosphate is cle
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32. Write short notes on anaerobic
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this phase is called lag phase. It
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Physiological effects of auxin Auxi
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endosperm of coconut. Cytokinin occ
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4. Bakanae disease in paddy is caus
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5.5. Photoperiodism and vernalizati
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c. sunflower d. maize 4. Which of t
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1. To develop more food crops 2. To
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Clonal selection Crops like sugarca
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The haploid individual plant will h
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methods new improved crops are obta
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6.2. Crop diseases and their contro
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Pathogen The mycelium of Cercospora
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3. They also bring about considerab
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It is hoped that one day geneticall
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2. The genetic resources of the dev
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This will affect millions of uneduc
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additives. It depends upon crop rot
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COMMONLY AVAILABLE MEDICINAL PLANTS
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urinary infections, tuberculosis, m
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Groundnut oil is used to a limited
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