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II. Answer the following questions in two or three sentences. 3. Define recombinant DNA. 4. Name the enzymes involved in the making of a DNA hybrid. 5. What are restriction endonucleases? 6. What is the importance of Escherichia coli in biotechnology? 7. What is the role of restriction enzymes in bacteria? 8. Define splicing. III. Answer the following questions in about 100 words. 9. What is a cloning vector? Why are they necessary? 10. Give an illustrated account of gene cloning. 11. What is the role of Agrobacterium for gene transfer in plants? 12. How is DNA cut? IV. Answer the following questions in about 200 words 13. Write an essay on DNA recombinant technology. 150
4.2 Transgenic plants Introduction of foreign gene In genetically engineered plant cells, a bacterium Agrobacterium is mainly involved in transfer of foreign gene. However, Agrobacterium cannot infect all plants since it has a narrow range of host specificity. Therefore other techniques have been developed to introduce foreign DNA into plant cells. Novel methods of ensuring DNA uptake into cells include electroporation and mechanical delivery or biolistics. Electroporation is a process of creating temporary pores in the cell membrane by application of electric field. Creation of such pores in a membrane allows introduction of foreign molecules such as DNA, RNA, antibodies, drugs, etc. into cytoplasm. The development of this technique is due to contribution of biophysics, bioengineering, cell and molecular biology. While the technique is now used widely to create transgenic microorganisms, plants and animals, it is used increasingly for application of gene therapy. The mechanical particle delivery or gene gun methods to deliver DNA on microscopic particles into target tissue or cells. The process is increasingly used to introduce new genes into a range of bacterial, fungal plant and mammalian species. It is the main method of choice for genetic engineering of many plant species including rice, corn, wheat, cotton and soyabean. Transgenic (modified) plants Presently, more than 50 types of genetically engineered plant species, called transgenic plants have been successfully developed. These plants were made to resist insect pests, viruses or herbicides through incorporation of foreign gene into DNA of host plant cells. Initially transgenic plants were developed more in dicotyledons, but now extended to several monocotyledons like wheat, maize, rice and oats. Transgenic plants have also been developed and are suitable for food industries (delaying ripening in tomato). Gene pharming, the use of transgenic plants as bioreactors or factories for production of speciality chemicals and pharmaceuticals is being pursued 151
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BOTANY Higher Secondary Second Year
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Preface The most important and cruc
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1. Taxonomy PRACTICALS (30 periods)
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1. TAXONOMY OF ANGIOSPERMS Taxonomy
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compared to perianth with two whorl
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a total variety or species. To conc
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well known system of classification
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Self Evaluation I . Choose and writ
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Series (iii) Calyciflorae It includ
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Class III Monocotyledonae Seeds of
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5. How many families were described
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Flower Bracteate or ebracteate, bra
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A twig Sepal Epicalyx Calyx A petal
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Self evaluation I . Choose and writ
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Inflorescence Usually a raceme eg.
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Calyx Ovary Gynoecium Bracteole Flo
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5. Fibre plants Fibres obtained fro
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1.3.3. RUBIACEAE - the coffee famil
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Stem Leaf Aerial, erect, branched,
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1. Beverage plants ECONOMIC IMPORTA
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1.3.4. ASTERACEAE - the sunflower f
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Habit Root Stem Leaf Botanical desc
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Fruit Seed A cypsela. Non-endosperm
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Flower Bracteate (eg. Petunia hybri
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Stamen Epipetalous stamens T.S. of
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(eg. Jatropha curcas). In xerophyti
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Perianth Tepals 5, arranged in sing
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1. Food plants ECONOMIC IMPORTANCE
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MONOCOT FAMILY 1.3.7 MUSACEAE - the
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Habit Root Stem Botanical descripti
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Androecium Stamens 6, in two whorls
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1.3.8. ARECACEAE - the palm family
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Habit Botanical description of Coco
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Gynoecium Absent but pistillode is
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II. Answer the following questions
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Classification of meristem Based on
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oval, spherical, rectangular, cylin
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Fibres Fibre cells are dead cells.
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Tracheid conducting elements in ang
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a lining layer of cytoplasm. This i
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2. Epidermis protects the underlyin
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made up of barrel shaped parenchyma
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2.2. Anatomy of monocot and dicot r
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The endodermal cells, which are opp
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Cortex Cortex consists of only pare
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II. Answer the following questions
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Ground plan A sector enlarged Fig.
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Ground plan A sector enlarged Fig.
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Xylem Xylem consists of xylem fibre
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2.4. Anatomy of a dicot and monocot
Page 105 and 106: Vascular tissues Vascular tissues a
Page 107 and 108: II. Answer the following questions
Page 109 and 110: 1. Formation of interfascicular cam
Page 111 and 112: Formation of periderm As secondary
Page 113 and 114: Each annual ring refers to one year
Page 115 and 116: 8. What are complementary cells? 9.
Page 117 and 118: chromosome has one centromere and t
Page 119 and 120: Chromosomal puff Dark band inter ba
Page 121 and 122: The genome size of an individual is
Page 123 and 124: ound pollen (bbll). All the F1 hybr
Page 125 and 126: points called chiasmata. Crossing o
Page 127 and 128: 3.4. Recombination of chromosome Th
Page 129 and 130: Point or gene mutation Point mutati
Page 133 and 134: 127 Fig. 3.6 Chromosomal aberration
Page 135 and 136: Autopolyploidy Ploidy - flow chart
Page 137 and 138: Self evaluation I . Choose and writ
Page 139 and 140: cells was injected into the mouse,
Page 141 and 142: Replication fork Super coil DNA pol
Page 143 and 144: 3.8. Structure of RNA and its types
Page 145 and 146: Role of RNA in protein synthesis Th
Page 147 and 148: The transfer of message present in
Page 149 and 150: IV. Answer the following questions
Page 151 and 152: to join DNA fragments. Thus the res
Page 153 and 154: Ti plasmid T-DNA Foreign DNA Bacter
Page 155: Restriction enzyme and ligase do no
Page 159 and 160: introducing Bt toxin gene in cotton
Page 161 and 162: 2. The number of transgenic plants
Page 163 and 164: Pot plant Explant Inoculation Surfa
Page 165 and 166: . Embryogenesis Formation of embryo
Page 169 and 170: Strain A Strain B Isolation of prot
Page 171 and 172: The term ‘single cell protein’
Page 173 and 174: 8. What is PEG? 9. How do you remov
Page 175 and 176: Many enzymes consists of a protein
Page 177 and 178: 5.1.3. Theories explaining the mech
Page 181 and 182: 5.2.1. Significance of photosynthes
Page 183 and 184: The reactions of photosynthesis can
Page 185 and 186: 179 Fig. 5.8. Non-cyclic photophosp
Page 187 and 188: Difference between cyclic and noncy
Page 189 and 190: power NADPH 2 . So, two molecules o
Page 191 and 192: Fig.5.11 Hatch-Slack pathway of CO
Page 193 and 194: Difference between C 3 and C 4 phot
Page 195 and 196: Glycine molecules enter into mitoch
Page 197 and 198: Among leaf factors, such as leaf ag
Page 199 and 200: Epiphyte eg. Vanda Aerial root Part
Page 201 and 202: When an insect is attracted by the
Page 203 and 204: 11. The gas evolved during photosyn
Page 205 and 206: 40. Explain the test tube and funne
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201 Fig. 5.17 Overall scheme of res
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Fig. 5.18 Process of Glycolysis 203
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decarboxylation and dehydrogenation
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9. The malic acid is oxidized to ox
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is fixed in the vertical position w
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Nonoxidative phase In this phase, v
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Respiratory quotient for anaerobic
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5. Fructose 1,6-bisphosphate is cle
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32. Write short notes on anaerobic
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this phase is called lag phase. It
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Physiological effects of auxin Auxi
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endosperm of coconut. Cytokinin occ
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4. Bakanae disease in paddy is caus
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5.5. Photoperiodism and vernalizati
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c. sunflower d. maize 4. Which of t
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1. To develop more food crops 2. To
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Clonal selection Crops like sugarca
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The haploid individual plant will h
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methods new improved crops are obta
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6.2. Crop diseases and their contro
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Pathogen The mycelium of Cercospora
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3. They also bring about considerab
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It is hoped that one day geneticall
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2. The genetic resources of the dev
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This will affect millions of uneduc
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additives. It depends upon crop rot
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COMMONLY AVAILABLE MEDICINAL PLANTS
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urinary infections, tuberculosis, m
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Groundnut oil is used to a limited
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