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BOTANY Higher Secondary Second Year - Textbooks Online

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specific for a particular amino acid. In bacterial cell, there are more than<br />

70 tRNAs and in eukaryotic cells the number is even greater. There are<br />

four or five tRNAs specific for a particular amino acid and these are<br />

called isoacceptor tRNAs.<br />

Structure<br />

The tRNA has a cloverleaf like structure. It is synthesized in the<br />

nucleus on a small part of DNA. In 1965, R.W. Holley suggested the<br />

cloverleaf model of tRNA. Though tRNA molecule consists of a single<br />

strand, it assumes clover leaf like structure through folding. There are<br />

three folds in the clover leaf tRNA. It has four arms namely anticodon<br />

arm, D arm, T C arm and aminoacid acceptor arm. The tRNA molecules<br />

are made up of 73 to 93 ribonucleotides. The acceptor arm carries an<br />

aminoacid. The anticodon arm has three anticodon nucleotides, which<br />

will join with the complementary codon in mRNA during protein synthesis<br />

i.e. three nucleotides in the tRNA pairs with three nucleotides of mRNA.<br />

In certain tRNAs in addition to these four arms an extra arm called variable<br />

arm occurs as shown in the figure. The aminoacid acceptor and the<br />

anticodon arms are oriented in opposite directions.<br />

Ribosomal RNA<br />

This is found in the ribosomes. The rRNA represents about 40 to 60<br />

per cent of the total weight of the ribosomes. Relatively it constitutes<br />

about 80 per cent of the total RNA of the cells. They are produced in the<br />

nucleus. They are the most stable forms of RNA. They consist of single<br />

strand of nucleotides. At some regions, the strand is folded.<br />

Comparison between DNA and RNA<br />

DNA RNA<br />

1. It contains a 5C deoxyribose. 1. It contains five carbon sugar ribose<br />

2. It contains adenine, guanine, 2. It contains adenine, guanine,<br />

cytosine and thymine cytosine and uracil.<br />

3. It mostly occurs as a double- 3. It often occurs as a single stranded.<br />

stranded helix.<br />

4. It is often much longer. 4. It is shorter.<br />

5. It is more stable. 5. It is less stable.<br />

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