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aided intrauterine deposition into the uterine lumen by-passing the cervix or<br />
transcervical insemination adapted to the anatomy of the ewe's genetalia.<br />
A new diluent named egg yolk lactose raffinose citrate glycerol (EYLRCG) has been<br />
evolved for pellet freezing of ram semen giving more than 50% post-thaw motlity. It contains<br />
18 ml eggyolk, 5g lactose, 8g raffinose, 2.2 g trisodium citrate dihydrate, 3.3 ml glycerol, 300<br />
mg streptopenicillin and 100 ml triple glass distilled water. Ejaculates of good quality semen<br />
are diluted with EYLRCG to bring the sperm concentration to 1 billion spermatozoa per ml.<br />
Diluted samples are equilibrated at 5°C for 4 h and then frozen into 0.2 ml size pellets.<br />
Pelleting is done in a pitted anodysed aluminium tray floating over liquid nitrogen. Pelleted<br />
semetlaced in goblets and stored in canisters by lowering under liquid nitrogen in cryocans.<br />
Thawing of pellets is done in dry test tubes individually upto liquification at 50°C.<br />
In order to overcome the disadvantages of proper identifi.cation of stored pellets and lack<br />
of repeatition in freezing conditions, a protocol has been developed, for deep freezing of semen<br />
in straws at CSWRI, Avikanagar. The freezing protocol, based on programmable freezing and<br />
computer assisted sperm analysis technique, gives 70% post-thaw motility consistently in all<br />
the breeds tested. Ejaculates having 90% initial motility and sperm density exceeding 3 billion<br />
spermatozoa per ml are extended in egg-yolk tris glycerol diluent to bring the final<br />
concentration to 1 billion spermatozoa per ml. The diluted samples are filled in 0.25 or 0.5 ml<br />
size straws, equilibrated for 2 h at 5°C and frozen @ -5 to -125°C per minute in a<br />
programmable cell freezer. Thawing is done at 50°C for 10 seconds in case of 0.25 ml size<br />
straws and at 60°C for 10 seconds in case of 0.5 ml straws in a water bath. The application of<br />
computer assisted sperm analysis technique during various stages of cryopreservation enables<br />
precise and objective evaluation of sperm function. Apart from identifying motile and immotile<br />
spermatozoa, it helps also in computing the sperm velocity and track dimensions of the motile<br />
spermatozoa. Efforts are also being made to identify factors causing cryo injury to ram<br />
spermatozoa during cryopreservation by cryo microscopic technique in order to amelio rate the<br />
sperm plasma membrane from the deleterious effect offreeze thaw process.<br />
7.3 Female reproduction<br />
Breeding ewes of indigenous breeds should be 1.5 to 2 years old depending upon their<br />
condition. Breeding too young ewes results in more wealclings and thus higher lamb losses.<br />
The ewes should posses a long preferably low set body, roomy hind-quarters, well formed<br />
pliable udder, active foraging habit and good mothering instinct. The ewes having poor milking<br />
capacity, overshot or undershot jaw, broken mouth, blind teat and meaty udder should be<br />
disqualified from the breeding programme. Body weight of an ewe at breeding should not<br />
normally be less than the adult body weight of that breed. The ewes should be prepared for<br />
mating when they are gaining weight, by giving them access to luxuriant pasture or feeding<br />
them a little grain for about 2-3 weeks preceding the beginning of the breeding season. After<br />
the ewes have been bred this extra feed can be stopped.<br />
The normal heat period in ewes lasts from 6 to 48 hours with an average of 24 hours. The<br />
normal oestrous cycle ranges from 16 to 19 days with an average of 17 days. The ewes come in<br />
heat usually about 2 months after lambing.<br />
<strong>Sheep</strong> can be bred naturally or through artificial insemination. The natural breeding is done<br />
by either flock mating, pen mating or hand mating. In flock-mating system artificial<br />
insemination makes possible the extensive use of superior exotic and outstanding<br />
crossbred/indigenous rams. On an average, a ram ejaculate measures about 1 ml and contains 3<br />
to 4 billion spermatozoa of which about 80-90 per cent are alive, if the semen is from a healthy<br />
ram. Semen of higher concentration is usually slightly acidic in reaction, and that of lower<br />
concentration slightly alkaline. In fertile semen, spermatozoa have a whirling motion. Semen<br />
can be stored for artificial insemination in a chilled liquid form or deep-frozen in liquid<br />
nitrogen. Preserving semen in a liquid form allows short-term preservation while the deepfrozen<br />
form permits 'long-term preservation for indenfinite period without any significant<br />
decrease in semen quality.<br />
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