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Sheep - AgRIS

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need for one or more "teasers" to detect which ewes are in estrus, and the frequent handling of<br />

the ewes during the breeding season.<br />

7 2. 2.1 Puberty<br />

Puberty in the males is marked by production of viable sperms and a desire for mating<br />

influenced by hormone testosterone. The age at puberty vary from 132 days for<br />

Tobasco (Pelibucy) x Dorset lambs on the high plane of nutrition in Mexico (Valecia et<br />

al., 1977)to as old as 738 days for 3/4 Rambouillet 1/4 Malpura lambs in Rajasthan<br />

(Tiwari and Sahni 1981). The quantity and quality of ejaculates collected from<br />

puberscent rams are poor with a high incidence of dead and deformed sper natozoa<br />

(EL-Wishy, 1974). Ram lambs reach puberty about two months earlier than ewe larnbs<br />

in each breed. Age at puberty depends upon the nutrition of lamb. Some breeders allow<br />

gimmers to mate immediately after they attain puberty. There is no evidence that early<br />

first lambing results in diminshed reproductive capacity (Wilson and Durkin, 1983).<br />

The hormonal stimulation of lambs is of practical use in the tropics. The gonads of<br />

prepubertal sheep respond to exogenous hormones, but animals stimulated in this way<br />

do not commence spontaneous estrous cycle (Hunter, 1980).<br />

7. 2.2.2 Ram Semen Collection<br />

Ram Semen can be collected by making use of (i) An Anoestrus ewe (ii) Artificial vagina<br />

(iii) by Electrical stimulation.<br />

i) Anoestrus ewe<br />

In this method the rams are trained to serve anoestrus ewe and after the service the semen<br />

is aspirated from the vagina. It is unsafe to use the semen so collected for fear of contamination.<br />

ii) Artificial vagina (A. V.)<br />

The pattern of A.V. is similar to that in the cattle, except that the A.V. is short. The outer<br />

hard rubber casing is 15.0 x 3.75 cm. The A.V. is prepared in the usual way by pouring hot<br />

water (45°C) through the inlet on the outer jacket. Necessary air is blown to provide cushioning<br />

affect and air valve tightened. Both the temperature and pressure are important. When the A.V.<br />

is ready, the inner temperature should be 40°C.<br />

Kaushish and Sahni (1976) considered it advisable to warrn the A.V. first with hot water<br />

(47°-50°V) initially and then pour out and refill the A.V. with hot water at 45° to 47°C,<br />

depending upon the season, in order to maintain the inner tube temperature between 40° - 41°C<br />

at the time of collection.<br />

A rubber cone is attached to one end of the A.V. at the end of which a graduated 5 ml test<br />

tube is fitted to serve as a receptacle for the semen. The open end of the A.V. should be<br />

properly lubricated preferably with liquid parafin. A glass collection cup may be directly fixed<br />

to the other end of the A.V. to obtain collection. Rams are very sensitive to temperature<br />

variations and therefore, adjusting, proper temperature in the A.V. is important. Once the rams<br />

are exposed either to an A.V. which is too hot or cold, they may refuse to serve on subsequent<br />

occasions. A freshly prepared artificial vagina should be used for each collection from a ram to<br />

avoid contamination. It should be properly sterilised aRer the use and kept in dry sterile<br />

chamber ready for use.<br />

iii) Electrical stimulation<br />

The equipment for collection of semen from the rams by electrical stimulation as modified<br />

by Dziuk et al., (1954) is said to be satisfactory. The power is obtained from 110 volt battery<br />

with 60 cycles which is reduced by transformer to 3 0 volt. This isolates the electrodes from the<br />

line of source. The probe for insertion into rectum is made up of rubber hose about 3/4 inch<br />

diameter with shoulder rings one inch apart. Alternate rings or electrodes are connected to<br />

provide the difference in electrical potential between adjacent electrodes. With this equipment<br />

the collection can be made in standing position. However, at times it is necessary to restrain the<br />

400

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