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Biological field and laboratory methods for measuring the quality of ...

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PLANKTON<br />

1.0 INTRODUCTION .<br />

2.0 SAMPLE COLLECTION AND PRESERVATION<br />

2.1 General Considerations . .<br />

2.1.1 Influential factors<br />

2.1.2 Sampling frequency<br />

2.1.3 Sampling locations<br />

2.1.4 Sampling depth<br />

2.1.5 Field notes<br />

2.1.6 Sample labelling<br />

2.2 Phytoplankton ....<br />

2.2.1 Sampling equipment<br />

2.2.2 Sample volume<br />

2.2.3 Sample preservation<br />

2.3 Zooplankton .<br />

2.3.1 Sampling equipment<br />

2.3.2 Sample volume<br />

2.3.3 Sample preservation<br />

3.0 SAMPLE PREPARATION<br />

3.1 Phytoplankton ...<br />

3.1.1 Sedimentation<br />

3.1. 2 Centrifugation<br />

3.1.3 Filtration<br />

3.2 Zooplankton ....<br />

4.0 SAMPLE ANALYSIS ..<br />

4.1 Phytoplankton ...<br />

4.1.1 Qualitative analysis <strong>of</strong> phytoplankton<br />

4.1.2 Quantitative analysis <strong>of</strong> phytoplankton<br />

4.2 Zooplankton . . . . . . . . . . . . . . . .<br />

4.2.1 Qualitative analysis <strong>of</strong> zooplankton<br />

4.2.2 Quantitative analysis <strong>of</strong> zooplankton<br />

5.0 BIOMASS DETERMINAnON<br />

5.1 Dry <strong>and</strong> Ash-Free Weight<br />

5.1.1 Dry weight<br />

5.1.2 Ash-free weight<br />

5.2 Chlorophyll .....<br />

5.2.1 In vitro measurements<br />

5.2.2 In vivo measurement<br />

5.2.3 Pheophytin correction<br />

5.3 Cell Volume .<br />

5.3.1 Microscopic (algae <strong>and</strong> bacteria)<br />

5.3.2 Displacement (zooplankton)<br />

5.4 Cell Surface Area <strong>of</strong> Phytoplankton<br />

6.0 PHYTOPLANKTON PRODUCTIVITY<br />

6.1 Oxygen Method<br />

6.2 Carbon-I 4 Method<br />

7.0 REFERENCES<br />

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