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Biological field and laboratory methods for measuring the quality of ...

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BIOASSAY<br />

1.0 GENERAL CONSIDERATIONS<br />

2.0 PHYTOPLANKTON - ALGAL ASSAY<br />

2.1 Principle .<br />

2.2 Planning Algal Assays<br />

2.3 Apparatus <strong>and</strong> Test Conditions<br />

2.3.1 Glassware<br />

2.3.2 Illumination<br />

2.3.3 pH<br />

2.4 Sample Preparation<br />

2.5 Inoculum<br />

2.6 Growth Response Measurements<br />

2.7 Data Evaluation . . . . . .<br />

2.8 Additions (Spikes) . . . . .<br />

2.9 Data Analysis <strong>and</strong> Interpretation<br />

2.10 Assays to Determine Toxicity<br />

3.0 PERIPHYTON<br />

3.1 Static . . . . . . .<br />

3.2 Continuous Flow<br />

4.0 MACROINVERTEBRATES<br />

5.0 FISH<br />

6.0 REFERENCES . . . . .<br />

6.1 General . . . . . .<br />

6.2 Phytoplankton - Algal Assay<br />

6.3 Periphyton<br />

6.4 Macroinvertebrates<br />

6.5 Fish .....<br />

FATHEAD MINNOW CHRONIC TEST<br />

BROOK TROUT CHRONIC TEST<br />

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