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TEST INSTRUCTIONS - Geotech Environmental Equipment

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DISSOLVED OXYGEN<br />

WINKLER COLORIMETRIC METHOD CODE 3688-SC<br />

QUANTITY CONTENTS CODE<br />

30 mL *Manganese Sulfate Solution *4167-G<br />

30 mL *Alkaline Potassium Iodide Azide *7166-G<br />

30 mL *Sulfuric Acid 1:1 *6141WT-G<br />

1 Sample Tube, screw cap 29180<br />

1 Cap 28570<br />

*WARNING: Reagents marked with an * are considered hazardous substances. To<br />

view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS CD<br />

or our web site. To obtain a printed copy, contact us by e-mail, phone or fax.<br />

Dissolved oxygen is vital to the survival of aquatic organisms. Naturally<br />

present, dissolved oxygen enters the water when plants photosynthesize. Wind<br />

and wave action also cause oxygen from the air to dissolve into water.<br />

Dissolved oxygen is consumed by aquatic animals and by the oxidation, or<br />

chemical breakdown, of dead and decaying plants and animals. The<br />

concentration of dissolved oxygen in natural waters can range from 0 to 14<br />

ppm and is effected by temperature and salinity.<br />

APPLICATION: This method is applicable for the determination of dissolved<br />

oxygen in drinking water, all surface waters and wastewater.<br />

RANGE: 0.0–11.0 Dissolved Oxygen<br />

METHOD: This method uses the azide modification of the Winkler<br />

Method with a colorimetric determination of the yellow<br />

iodine produced from the reaction with the dissolved<br />

oxygen.<br />

INTERFERENCES: The presence of other oxidizing agents may cause positive<br />

interferences. Reducing may cause negative interferences.<br />

Nitrite interferences are eliminated with the azide<br />

modification.<br />

Smart2 <strong>TEST</strong> PROCEDURES 2.04 Dissolved Oxygen 1/3

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