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TEST INSTRUCTIONS - Geotech Environmental Equipment

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AMMONIA-NITROGEN - LOW RANGE<br />

SALICYLATE METHOD CODE 3659-01-SC<br />

QUANTITY CONTENTS CODE<br />

60 mL *Salicylate Ammonia #1 *3978-H<br />

10 g *Salicylate #2 *7457-D<br />

2x5g *Salicylate#3 *7458-C<br />

1 Spoon, 0.1 g, plastic 0699<br />

1 Spoon, 0.15 g, plastic 0727<br />

1 Pipet, 1.0 mL, plastic 0354<br />

*WARNING: Reagents marked with an * are considered hazardous substances.<br />

To view or print a Material Safety Data Sheet (MSDS) for these reagents see<br />

MSDS CD or our web site. To obtain a printed copy, contact us by e-mail,<br />

phone or fax.<br />

Ammonia nitrogen is present in various concentrations in many surface and<br />

ground water supplies. Any sudden change in the concentration of ammonia<br />

nitrogen in a water supply is cause for suspicion. A product of microbiological<br />

activity, ammonia nitrogen is sometimes accepted as chemical evidence of<br />

pollution when encountered in natural waters.<br />

Ammonia is rapidly oxidized in natural water systems by special bacterial<br />

groups that produce nitrite and nitrate. This oxidation requires that dissolved<br />

oxygen be available in the water. Ammonia is an additional source of nitrogen<br />

as a nutrient which may contribute to the expanded growth of undesirable<br />

algae and other forms of plant growth that overload the natural system and<br />

cause pollution.<br />

APPLICATION: Low concentrations of ammonia in fresh, brackish and salt<br />

water; fresh and salt water aquariums.<br />

RANGE: 0.00 - 1.00 ppm Ammonia-Nitrogen<br />

METHOD: Salicylate and ammonia react at high pH in the presence of<br />

a chlorine donor and an iron catalyst to form a blue<br />

indophenol dye, the concentration of which is proportional<br />

SAMPLE<br />

HANDLE &<br />

PRESERVATION:<br />

to the ammonia concentration in the sample.<br />

Ammonia solutions tend to be unstable and should be<br />

analyzed immediately. Samples may be stored for 24 hours at<br />

4°C or 28 days at –20°C.<br />

INTERFERENCES: There are few interferences in most natural waters. High<br />

concentrations of reducing agents, such as hydrazine, react<br />

with the chlorine donor and can result in negative<br />

interferences. Color and turbidity can also interfere.<br />

Smart2 <strong>TEST</strong> PROCEDURES 2.04 Ammonia-Nitrogen - LR 1/3

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