VITAMIN A FORTIFIed PEANUT BUTTER - The Official Website of ...

VITAMIN A FORTIFIed PEANUT BUTTER - The Official Website of ... VITAMIN A FORTIFIed PEANUT BUTTER - The Official Website of ...

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(1983) as cited by Solon et al. (1985), an average of 76% vitamin A is lost after 6 mos of storage. Fortified peanut butter was shown to lose 12% of vitamin A after 180 days of storage at 80°F (Willich et al., 1954). Peanut spread with nonfat dairy milk fortified with vitamin A only retained 30% vitamin A after 3 mos of storage in the dark at 23 or 40°C (Yeh et al., 2002). About one-half of the amounts of vitamin A added to fortified rice were lost after 24 wks of storage at 35°C (Lee et al, 2000). Storage of cornflakes fortified with vitamin A palmitate at 23 and 45°C for 6 to 8 weeks resulted in more than 90% loss in vitamin A. Influence of Stabilizer The amount of vitamin A retained (as % of RENI) in stabilized peanut samples was significantly affected by the brands of stabilizers used (Table 3.3). All stabilized peanut butter samples with Fix-X generally showed higher amounts of vitamin A except for samples with microencapsulated vitamin A palmitate fortified at 70% and 140% and beta-carotene at 140% RENI, than samples with Myvatex monoset®. The type of fortificant used and the level of fortification significantly influenced the effects of stabilizers on vitamin A retained after processing of peanut butter. Peanut butter samples fortified with vitamin A palmitate (oily preparation and microencapsulated) and stabilized with the two brands of stabilizers did not significantly differ in terms of vitamin A retained after processing (Table 3.5). However, peanut butter samples fortified with betacarotene and stabilized with Fix X contained higher amounts of vitamin A than samples stabilized with Myvatex monoset®. Likewise the combined effects of the level of fortification and the brand of stabilizer significantly affected the amount of vitamin A retained in peanut butter samples with beta-carotene. Table 3.4 Analysis of Variance (ANOVA) of overall effects and interaction of factors and means of the factors studied on the percent vitamin A recovered in fortified stabilized peanut butter after processing Type of fortificant: Vitamin A (Oily Preparation) Vitamin A (Microencapsulated) 10% Beta-carotene Level of fortificant 2 70% of RENI 140% of RENI 210% of RENI Factors % Recovery Means 1 46.88c 54.38b 89.43a 71.22a 61.76b 57.71c Stabilizer brand: Myvatex monoset® 3 Fix-X 4 63.13 64.00 1 Means within each factor followed by the same letters are not significantly different at 5% level. 2 Based on the RENI for Filipino male adult= 525 μg RE 3 Local brand 4 Imported brand 5 Significant at 5% level p-value 0.0001 5 0.0019 5 0.7555 93

Table 3.5 Analysis of the individual effects of the level of fortification (ANOVA) and brand of stabilizer (t-Test) on the amount of vitamin A retained in stabilized peanut butter after processing per fortificant used Factors A. Level of fortification 2 70% RENI 140% RENI 210% RENI B. Brand of stabilizer (t-Test) Vitamin A Palmitate (oily preparation) Means 1 38.10b 63.50b 98.01a p-value Means 1 0.0081 5 0.6375 Vitamin A Palmitate (microencapsulated) Beta-carotene (10%) p-value Means 1 p-value 32.76c 49.10b 63.96a 0.0007 5 0.9523 67.34c 137.18b 261.56a Myvatex monoset® 3 Fix-X 4 64.05 48.50 120.09b 69.03 48.71 190.62a 1 Means within each factor in a column followed by the same letters are not significantly different at 5% . 2 Based on the RENI for Filipino male adult= 525 μg RE 3 Local brand 4 Imported brand 5 Significant at 5% level 0.0001 5 0.0001 5 Although higher (36.57-175.37% RENI) amount of vitamin A was retained in peanut butter samples stabilized with Fix X, as discussed previously, all samples retained at least one-third of RENI of vitamin A. Moreover, since the % vitamin A retention in all stabilized peanut butter samples did not significantly differ (Table 3.6), this demonstrates that Myvatex monoset® can be substituted to Fix X in stabilized peanut butter fortified with vitamin A that will give at least 1/3 of RENI. The study by Chapman et al. (1998) on vitamin A degradation in chocolate milk stabilized with carrageenan, showed that colloidal suspensions effectively scattered light, thus, minimized photooxidation of vitamin A. Stabilization of the peanut butter with the stabilizers containing rapeseed and cottonseed oils was shown to be through a fat crystal network or matrix, which held peanut solids in suspension (Aryana et al., 2000). This matrix may also help prevent photooxidation of vitamin A in the product upon storage. 94

(1983) as cited by Solon et al. (1985), an average <strong>of</strong> 76% vitamin A is lost after 6 mos <strong>of</strong> storage.<br />

Fortified peanut butter was shown to lose 12% <strong>of</strong> vitamin A after 180 days <strong>of</strong> storage at 80°F (Willich et<br />

al., 1954). Peanut spread with nonfat dairy milk fortified with vitamin A only retained 30% vitamin A<br />

after 3 mos <strong>of</strong> storage in the dark at 23 or 40°C (Yeh et al., 2002). About one-half <strong>of</strong> the amounts <strong>of</strong><br />

vitamin A added to fortified rice were lost after 24 wks <strong>of</strong> storage at 35°C (Lee et al, 2000). Storage <strong>of</strong><br />

cornflakes fortified with vitamin A palmitate at 23 and 45°C for 6 to 8 weeks resulted in more than 90%<br />

loss in vitamin A.<br />

Influence <strong>of</strong> Stabilizer<br />

<strong>The</strong> amount <strong>of</strong> vitamin A retained (as % <strong>of</strong> RENI) in stabilized peanut samples was significantly<br />

affected by the brands <strong>of</strong> stabilizers used (Table 3.3). All stabilized peanut butter samples with Fix-X <br />

generally showed higher amounts <strong>of</strong> vitamin A except for samples with microencapsulated vitamin A<br />

palmitate fortified at 70% and 140% and beta-carotene at 140% RENI, than samples with Myvatex<br />

monoset®. <strong>The</strong> type <strong>of</strong> fortificant used and the level <strong>of</strong> fortification significantly influenced the effects <strong>of</strong><br />

stabilizers on vitamin A retained after processing <strong>of</strong> peanut butter.<br />

Peanut butter samples fortified with vitamin A palmitate (oily preparation and<br />

microencapsulated) and stabilized with the two brands <strong>of</strong> stabilizers did not significantly differ in terms <strong>of</strong><br />

vitamin A retained after processing (Table 3.5). However, peanut butter samples fortified with betacarotene<br />

and stabilized with Fix X contained higher amounts <strong>of</strong> vitamin A than samples stabilized with<br />

Myvatex monoset®. Likewise the combined effects <strong>of</strong> the level <strong>of</strong> fortification and the brand <strong>of</strong> stabilizer<br />

significantly affected the amount <strong>of</strong> vitamin A retained in peanut butter samples with beta-carotene.<br />

Table 3.4 Analysis <strong>of</strong> Variance (ANOVA) <strong>of</strong> overall effects and interaction <strong>of</strong> factors and<br />

means <strong>of</strong> the factors studied on the percent vitamin A recovered in fortified stabilized<br />

peanut butter after processing<br />

Type <strong>of</strong> fortificant:<br />

Vitamin A (Oily Preparation)<br />

Vitamin A (Microencapsulated)<br />

10% Beta-carotene<br />

Level <strong>of</strong> fortificant 2<br />

70% <strong>of</strong> RENI<br />

140% <strong>of</strong> RENI<br />

210% <strong>of</strong> RENI<br />

Factors % Recovery<br />

Means 1<br />

46.88c<br />

54.38b<br />

89.43a<br />

71.22a<br />

61.76b<br />

57.71c<br />

Stabilizer brand:<br />

Myvatex monoset® 3<br />

Fix-X 4<br />

63.13<br />

64.00<br />

1<br />

Means within each factor followed by the same letters are not significantly different at 5% level.<br />

2<br />

Based on the RENI for Filipino male adult= 525 μg RE<br />

3<br />

Local brand<br />

4<br />

Imported brand<br />

5<br />

Significant at 5% level<br />

p-value<br />

0.0001 5<br />

0.0019 5<br />

0.7555<br />

93

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