VITAMIN A FORTIFIed PEANUT BUTTER - The Official Website of ...

mixer. The final fortified peanut butter was manually filled in plastic containers, sealed and kept at
refrigerated conditions. The schematic diagram of the procedure for the preparation of fortified peanut
butter at FDC is shown in Fig. 2.5. The plain peanut butter and premix used in this study were prepared
following Figures 2.1 and 2.4, respectively.
Weigh required amounts of premix and plain peanut butter
in separate containers according to the required
premix to plain peanut butter proportion
Add pre-weighed premix to the center of
pre-weighed plain peanut butter
Manually mix the premix to plain peanut butter with a plastic
spatula for approximately 1 min or until no trace
of the premix is visible at the surface of the mixture
Transfer the premix-plain peanut butter mixture to
a Hobart vertical mixer Model HCM 300
Mix for 5 min
Scrape off adhering peanut butter from the sides of the mixer
Mix for another 5 min
Manually fill the fortified peanut butter in plastic containers
Seal containers and store at refrigerated conditions
Fig. 2.5 Schematic diagram of procedure for the laboratory scale preparation of fortified peanut
butter at FDC.
Experiment 1: Validation of the adequacy of the ten minute mixing time established in the dye
test.
Experiments were conducted to validate the adequacy of the 10 min mixing time established in
the dye test, for the dispersion of the fortificant in the premix and of the premix in the final fortified
product. Adequacy of the mixing time was evaluated based on % recovery of vitamin A in the premix
and in the final fortified product and % variability of replicate samples. Percent recovery and %
variability were obtained using the following formula:
% Recovery = Vitamin A content in the product after processing x 100
Expected level of vitamin A in the product
% Variability = Average vitamin A in replicate samples x 100
Standard deviation of replicate samples
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