VITAMIN A FORTIFIed PEANUT BUTTER - The Official Website of ...

until no trace of the fortificant was visible at the surface of the peanut butter. The mixture was then
transferred to a Hobart vertical mixer model HCM 300 (Hobart Corporation, Troy, Ohio, U.S.A.) and
mixed for a total of 10 min stopping every 5 min interval to allow retrieval of adhering peanut butter from
the sides of the mixer. The resulting premix was manually filled into plastic containers, sealed and stored
at refrigerated conditions until its intended use. The schematic diagram of the procedure for the
laboratory preparation of peanut butter premix at FDC is shown in Fig. 2.4. Plain peanut butter used in
this study was prepared following the procedure of Galvez et al. (2000) described in Fig. 2.1.
Pre-heat vitamin A palmitate in a water bath
maintained at 40 o C for 15 min
Weigh required amounts of vitamin A palmitate
and plain peanut butter in separate containers
Add pre-weighed vitamin A palmitate to the center
of pre-weighed plain peanut butter
Manually mix the vitamin A palmitate to plain peanut butter
with a plastic spatula until no trace of the fortificant
is visible from the surface of the peanut butter mixture
Transfer the vitamin A palmitate-peanut butter mixture to
a Hobart vertical mixer Model HCM 300
Mix for 5 min
Scrape off adhering peanut butter from the sides
of the mixer and add back to the mixture
Mix for another 5 min
Manually fill the premix in plastic containers
Seal containers and store in refrigerated conditions
Fig. 2.4 Schematic diagram of the procedure for the laboratory scale preparation of premix at
FDC.
Laboratory Procedure for the Preparation of Fortified Peanut Butter at FDC
Fortified peanut butter was prepared by adding premix to the center of plain peanut butter in
amounts that varied depending on the actual vitamin A content of the peanut butter premix used. The
minimum target fortification level of the final fortified product was 4.38 µg RE/g peanut butter. The
premix and plain peanut butter were mixed manually with a plastic spatula until no trace of the premix
was visible at the surface of the mixture. The premix-plain peanut butter mixture was then transferred to
a Hobart vertical mixer Model HCM 300 (Hobart Corporation, Troy, Ohio, U.S.A.) and mixed for a total
of 10 min, stopping every 5 min interval to allow retrieval of adhering product from the sides of the
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