VITAMIN A FORTIFIed PEANUT BUTTER - The Official Website of ...

Prepare dyed peanut butter by adding 1 ½ tablespoons
of annatto powder to plain peanut butter
until the color changes to reddish orange
Weigh required amounts of dyed peanut butter and
plain peanut butter in separate containers
Transfer the plain peanut butter to a
Hobart vertical mixer Model HCM 300
Add the dyed peanut butter directly
to the center of plain peanut butter
Mix for 5 min
Observe the sides and bottom of the mixer for presence
of reddish orange streaks indicative of the dye not
yet fully dispersed with peanut butter mixture
Scrape off sides of mixer for adhering
peanut butter and add back to mixture
Mix the peanut butter mixture for another
5 min in the same mixer
Observe the peanut butter mixture for disappearance of reddish orange
streaks in the mixture and at the sides and bottom of the mixer
Fig. 2.3 Schematic diagram of the dye test procedure for the addition of dyed peanut butter to
plain peanut butter
Laboratory Trials at FDC
Laboratory trials were conducted at FDC to establish the level of vitamin A addition in the
premix and in the final fortified product. Fortified peanut butter was prepared using a two-step
fortification process that involved the preparation of a premix followed by the preparation of the final
fortified product. The procedures for the preparation of premix and preparation of fortified peanut butter
are described as follows:
Laboratory Procedure for the Preparation of Peanut Butter Premix at FDC
Prior to use, the fortificant (vitamin A palmitate in oil) was pre-heated at 40 o C for 15 min in a
water bath as recommended by the supplier (BASF, 1997). The required amounts of fortificant and plain
peanut butter were weighed in separate containers after which the pre-weighed fortificant was added to
the center of pre-weighed plain peanut butter. The mixture was manually mixed with a plastic spatula
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