VITAMIN A FORTIFIed PEANUT BUTTER - The Official Website of ...

VITAMIN A FORTIFIed PEANUT BUTTER - The Official Website of ... VITAMIN A FORTIFIed PEANUT BUTTER - The Official Website of ...

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Materials needed: Appendix D (Name of Company) SAMPLING AND EVALUATION OF WATER FOR MICROBIOLOGICAL QUALITY Sterile polyethylene bags to contain at least 300 grams of ice Sterile bottles with cover to contain at least 300 mL water Alcohol lamp Denatured alcohol Match Rubbing alcohol (for disinfecting hands) Cotton balls A pair of tongs or forceps A piece of clean cloth (for wiping top) A styropore box and ice for keeping samples Procedure: 1. Ice sample a. Use an inverted sterile polyethylene bag as hand glove. b. Place ice sample (approximately 300 g) in double layer sterile polyethylene bag and seal. c. Keep refrigerated until analyzed. 2. Water sample a. Choose tap water which is directly connected with the main supply. b. Turn on the tap and allow water to run waste for 2-3 minutes. Then turn off. Dry the outer surface with a clean cloth. c. Sterilize the tap. c.1 Moisten a piece of cotton with denatured alcohol. c.2 Ignite and hold it with a pair of tongs (or forceps). c.3 Flame the top. d. Cool the tap by allowing water to run to waste for a few seconds. e. Open sample bottle. It should be held by the base in one hand while the cover is held by the other. f. Fill the bottle from a gentle stream of water. Avoid splashing. During the whole procedure, the cover and the mouth of the bottle should not be allowed to touch anything. g. Flame mouth of the bottle, then replace cap. h. Keep sample in refrigerated container during transport. 191

Appendix 4 continued… Important points to remember when collecting samples: 1. Sample container should be sterile and should be kept unopened until it is required for filling. 2. The faucet should be clean. 3. Hands of the one collecting sample should be clean. 4. Care should be taken to avoid accidental contamination during collection. 5. Sample should be representative of the water to be examined. 6. The changes which occur in the bacterial content of samples can be reduced by transporting the sample to the laboratory in a refrigerated container. 7. Time between collection and examination of samples should be within six hours. It is important, however, that samples should be examined as soon as possible after collection. 192

Materials needed:<br />

Appendix D<br />

(Name <strong>of</strong> Company)<br />

SAMPLING AND EVALUATION OF WATER<br />

FOR MICROBIOLOGICAL QUALITY<br />

Sterile polyethylene bags to contain at least 300 grams <strong>of</strong> ice<br />

Sterile bottles with cover to contain at least 300 mL water<br />

Alcohol lamp<br />

Denatured alcohol<br />

Match<br />

Rubbing alcohol (for disinfecting hands)<br />

Cotton balls<br />

A pair <strong>of</strong> tongs or forceps<br />

A piece <strong>of</strong> clean cloth (for wiping top)<br />

A styropore box and ice for keeping samples<br />

Procedure:<br />

1. Ice sample<br />

a. Use an inverted sterile polyethylene bag as hand glove.<br />

b. Place ice sample (approximately 300 g) in double layer sterile polyethylene bag and seal.<br />

c. Keep refrigerated until analyzed.<br />

2. Water sample<br />

a. Choose tap water which is directly connected with the main supply.<br />

b. Turn on the tap and allow water to run waste for 2-3 minutes. <strong>The</strong>n turn <strong>of</strong>f. Dry the outer surface<br />

with a clean cloth.<br />

c. Sterilize the tap.<br />

c.1 Moisten a piece <strong>of</strong> cotton with denatured alcohol.<br />

c.2 Ignite and hold it with a pair <strong>of</strong> tongs (or forceps).<br />

c.3 Flame the top.<br />

d. Cool the tap by allowing water to run to waste for a few seconds.<br />

e. Open sample bottle. It should be held by the base in one hand while the cover is held by the other.<br />

f. Fill the bottle from a gentle stream <strong>of</strong> water. Avoid splashing. During the whole procedure, the<br />

cover and the mouth <strong>of</strong> the bottle should not be allowed to touch anything.<br />

g. Flame mouth <strong>of</strong> the bottle, then replace cap.<br />

h. Keep sample in refrigerated container during transport.<br />

191

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