VITAMIN A FORTIFIed PEANUT BUTTER - The Official Website of ...

The amount of vitamin A (as % of RENI) for both levels of fortification increased as compared to
previous chocolate-peanut spreads fortified at a level of 100% of RENI. However, the target level of 1/3
of the RENI (or 33% of RENI) was not met by both fortification rates. Moreover, the higher fortification
level of 1 2/3 resulted in lower amount of vitamin A retained. T-test analysis for amount of vitamin A
(as % of RENI) showed no significant differences between the two fortification rates used (Table 4.5).
The same trend was also observed for % retention of vitamin A. Values were found to be
significantly different from each other. Results show that increasing the fortification level equivalent to
166% (1 2/3 of RENI) of RENI was still not sufficient to meet the target RENI. Consistency of the
mixture was very viscous, such that, homogenous distribution of the fortificant throughout the sample
was greatly affected. The possibility that the fortificant was not homogeneously distributed in the product
can be seen in the data were retention decreased as the level of fortification was increased. The low
values obtained may actually be due to problems related to the inability of the process to ensure the
homogeneous distribution of the fortificant in the product. This implies then that the fortification
procedure established from previous studies cannot be adapted for the chocolate-peanut spread. Further
studies need to be conducted regarding the delivery of the fortificant into the mixture.
Table 4.4 Percent Vitamin A recovered 1 and amount of vitamin A (based on % RENI) retained
after processing of Chocolate-Peanut Spread fortified with vitamin A at different
levels of fortification using vitamin A palmitate (microencapsulated) 2
Level of Fortification 3
133% of RENI
Amount of Vitamin A
% of RENI retained % Recovery
23.20
17.40
166% of RENI
1
Means of three replicates.
21.60 13.02
2
Purity of vitamin A palmitate (microencapsulated)=246,256.10 IU/g
3
Based on the RENI for Filipino male adult=525 μg RE
Table 4.5 t-Test analysis of means on the % Vitamin A (based on % RENI and recovery) in
Chocolate-Peanut spread after processing
Variable Means 1 p-value
% of RENI 2
1 1/3 of RENI
1 2/3 of RENI
23.20
21.60
0.3755
0.0304 3
% Recovery
1 1/3 of RENI
17.40a
1 2/3 of RENI
13.02b
1
Means within each factor followed by different letters are not significantly different at 5% level.
2
Based on the RENI for Filipino male adult=525 μg RE
3
Significant at 5% level
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