THE PHILIPPINE WATER BUFFALO

on the factors affecting embryo recovery and transfer, physiology
and anatomy of the reproductive tract of the water buffalo.
Keywords: non-surgical embryo recovery, river buffaloes,
swamp buffalo
*Jpn. J. Vet. Res. 1988. 36:257-263. Also in Abstracts of
Water Buffalo Researches in the Philippines 1981-1995.
1996. PCC-DA. p. 64-65. Also in Annotated Bibliography
on Philippine Biodiversity: Livestock and Poultry
(Agrobiodiversity) 1949-1997, Carabaos. 2002. p. 340.
R-47
STUDY ON SOME ASPECTS OF REPRODUCTIVE
PERFORMANCE OF ESTRUS SYNCHRONIZED
PHILIPPINE CARABAO (Bubalus bubalis Linn.) IN
SELECTED TOWNS OF NUEVA ECIJA*
W. A. Pascua, Undergraduate Student, DAS-CA, CLSU
F. V. Mamuad, Center Director, PCC-CLSU, DA
Records on estrus synchronization and artificial insemination
in water buffalo in selected towns of Nueva Ecija, filed at the
Philippine Carabao Cener (PCC) at Central Luzon State
University Ranch Joson (Digdig, Caranglan, Nueva Ecija)
were analyzed and evaluated. Some aspects of reproductive
performace of estrus synchronized Philippine carabao were
determined. These following observations were divided into
site of deposition, appearance of the vulva, tone of the uterus,
body condition, and time of insemination and number of
services per calving. Results showed that Philippine Carabao
has a higher calving at first srvice (35.38 percent), while at
the second service it has a 18.46 percent calving rate, a total
of 70 carabao calved out of 130 artificially inseminated (AI)
animals. Likewise, swollen vulva, a slightly hard uterus,
time of injecting PGF 2 alpha, time of AI after injection of PGF 2
alpha, body condition score of 3, and body of the uterus
were noted for having highest percentage of calving. Analysis
of the Philippine carabao in selected towns of Nueva, Ecija
showed that site of semen deposition was correlated to the
calving rate of the animals. Body condition score, appearance
of the vulva, tone of uterus, number. of services, and time of
insemination did not influence the calving rate of the animals.
Keywords: estrus synchronization, artificial insemination,
Philippine carabao, estrus signs, calving rate, body score,
reproductive performance
* Undergraduate Thesis. 2003. DAS-CA, CLSU. 38 p.
R-48
STUDY ON NON-SURGICAL RECOVERY TECH-
NIQUES IN WATER BUFFALO (Bubalus bubalis)
UNDER THE TO AND FRO SYSTEM*
REPRODUCTION
J. F. Pastor, Student, DAS-CA, CLSU
J. Eyadan, Professor, DAS-CA, CLSU
E. Atabay, Science Research Specialist, PCRDC-PCARRD, CLSU
H. Venturina, Science Research Specialist, PCRDC-PCARRD, CLSU
P. Duran, Science Research Specialist, PCRDC-PCARRD, CLSU
C. Valdez, Student, Hokkaido University
R. Uenishi, M.S. Student, Hokkaido University
M. B. Ocampo, Ph.D. Student, Hokkaido University
H. Kanagawa, Professor, JFVM, Hokkaido University
L. C. Cruz, Prof., DAS-CA, CLSU and Project Leader, PCRDC-PCARRD,
CLSU
Three river buffaloes and one swamp buffalo were used in
this study. Selection of these animals were based on the
record of their reproductive performance. Superovulation
was carried out by giving each buffalo a first injection of 25
mg prostaglandin F 2 alpha (PGF 2 alpha) followed by 2000
IU of pregnant mare serum gonadotropin (PMSG) 10 days
after. A second dose of PGF 2 alpha was given 48 hours
after PMSG injection. After 72 hours, the buffaloes came
into heat and GnRH was injected at 250 mg per head.
Confirmation of estrus was performed by the use of a
teaser bull, and artificial insemination was carried out. The
number of ovulation was estimated by counting the corpora
lutea via rectal palpation. Flushing of each uterine horn for
the collection of embryos was carried out at approximately
4.5 days after insemination. Morula and an unfertilized
oocyte were recovered from the right and left uterine horns,
respectively.
Keywords: reproductive performance, river buffaloes,
swamp buffalo, superovulation
*Abstracts of Water Buffalo Researches in the Philippines.
1981-1995. 1996. PCC-DA. p. 68. Also in Annotated
Bibliography on Philippine Biodiversity: Livestock and
Poultry (Agrobiodiversity) 1949-1997, Carabaos. 2002. p.
343.
R-49
PRELIMINARY STUDY ON SUPEROVULATION AND
NON-SURGICAL EMBRYO RECOVERY TECHNIQUE
IN WATER BUFFALO*
J. F. Pastor, Student, DAS-CA, CLSU
J. Eyadan, Professor, DAS-CA, CLSU
M. B. Ocampo, Ph.D. Student, Hokkaido University
C. Valdez, Student, Hokkaido University
R. Uenishi, M.S. Student, Hokkaido Uniersity
E. C. Atabay, Science Research Specialist, PCRDC, PCARRD
H. V. Venturina, Science Research Specialist, PCRDC-PCARRD
P. G. Duran, Science Research Specialist, PCRDC-PCARRD
H. Kanagawa, Professor, JFVM, Hokkaido University
L. C. Cruz, Professor, DAS-CA, CLSU and Project Leader, PCRDC-PCARRD
Three river buffaloes and one swamp buffalo in luteal phase
were used to test their ovulatory response to PMSG,
PGF 2 alpha and GnRH treatments. Collection of embryos
81