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THE PHILIPPINE WATER BUFFALO

THE PHILIPPINE WATER BUFFALO

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after the second injection. The conception rate of 20 induced<br />

animals was 55% against 15% for a control group of 20<br />

normally cycling (uninduced) carabaos. However, the<br />

difference in conception incidence between two groups<br />

was not statistically significant (probability of calculated<br />

chi-square = 70). By manner of insemination, induced<br />

animals had a conception rate of 66.7% (8 of 12) for natural<br />

service and 40% (3 of 8) for artificial insemination. Control<br />

(uninduced) carabaos had 80% (8 of 10) and 50% (5 of<br />

10) conception rates for natural and artificial insemination<br />

sub-groups, respectively.<br />

Keywords: caracows, conception rate, estrus induction<br />

and synchronization, prostaglandin F 2 -alpha<br />

*Philippine Journal of Veterinary Animal Science. 8(1&2):55<br />

(Abstr.). Also in Annotated Bibliography on Philippine<br />

Biodiversity: Livestock and Poultry (Agrobiodiversity)<br />

1949-1997, Carabaos. (2002) p. 250<br />

R-23<br />

ESTRUS PHENOMENA OF CARABAO UNDER<br />

RANGE MANAGEMENT CONDITION*<br />

F. V. Mamuad, Instructor, DAS-CA, CLSU<br />

P. G. Duran, Science Research Specialist, PCRDC-PCARRD, CLSU<br />

H. V. Venturina, Science Research Specialist, PCRDC-PCARRD, CLSU<br />

A study was conducted to establish the estrous cycle,<br />

duration of estrus and diurnal variations on the incidence of<br />

estrus symptoms of Philippine carabao (PC) raised under<br />

range condition. Females in the institutional herd exhibiting<br />

normal cycles were used in the study. They were exposed<br />

to two vasectomized bulls and observed round the clock.<br />

A total of 886 observations indicated that estrus usually<br />

start in the morning. Peak incidence of estrus was observed<br />

during the cooler part of the day from 5:00 a. m. to 9:00 a.<br />

m. The average percentage of caracows that exhibited<br />

estrus in the morning was 79.03% as against 20.83% for<br />

those that showed heat in the afternoon. Duration of estrus<br />

was from 12 to 36 hours with a mean value 23.24 + 3.7<br />

hours and repeated every 22.49 + 6.9 days. Observable<br />

signs of estrus manifestation were mounting other animals<br />

(38.17%), standing still while being mounted by teaser<br />

bull (22.9%), and copious mucus discharge (36.10%).<br />

Symptoms like swelling and reddening of the vulva,<br />

bellowing and restlessness were observed in only 2.73%<br />

of the animals.<br />

Keywords: Philippine carabao, estrus, range condition<br />

*Philippine Journal of Veterinary Animal Science. 10(3-<br />

4):46 (Abstr.). Also in Abstracts of Water Buffalo<br />

Researches in the Philippines 1981-1995. 1996. PCC-<br />

DA. p. 54. Also in Annotated Bibliography on Philippine<br />

REPRODUCTION<br />

Biodiversity: Livestock and Poultry (Agrobiodiversity)<br />

1949-1997, Carabaos (2002) p. 184.<br />

R-24<br />

OPTIMIZING HANDLING PROCESSING TECH-<br />

NIQUES FOR <strong>THE</strong> PRODUCTION OF QUALITY<br />

SEMEN II. PRELIMINARY STUDY ON <strong>THE</strong><br />

REVIVABILITY OF <strong>BUFFALO</strong> SPERMATOZOA<br />

FOLLOWING DIFFERENT FREEZING METHODS*<br />

T. Matsumoto, Expert in Semen Processing, Japan Overseas Corp. Volntr.<br />

(JOCV)<br />

F. V. Mamuad, Associate Professor, DAS-CA, CLSU<br />

F. P. Aquino, Science Research Assistant, PCRDC-PCARRD, CLSU<br />

R. O. Cuaresma, Science Research Specialist I, PCRDC-PCARRD, CLSU<br />

The study aimed to develop an alternative freezing method<br />

for processing buffalo semen of high quality, utilizing limited<br />

materials. Three freezing methods were used: T1=ice<br />

chest, T2=tank freezing A, and T3=tank freezing B. Murrah<br />

buffalo (MB) (n=7), Philippine carabao (PC) (n=2), and F 1<br />

crossbred (MB x PC) (n=4) were used in the study. All<br />

ejaculates were diluted with Tris-yolk-raffinose glycerol<br />

extender up to a concentration of 100 million motile sperm<br />

per ml. Two freezing trials were made. First trial was with<br />

the use of T 2 versus T 1 . Second trial compared T 1 with that<br />

of T 3 . Results showed that T 2 gave higher post freezing<br />

revivability with 52.06 +11.39% than T 1 with 36.18% for<br />

all breeds tested. Similarly, postfreezing revivability of<br />

buffalo spermatozoa in T 3 was 53.30% +10.20%, which<br />

was higher that that of T 1 . Variation on percent revivability<br />

showed minimal differences among different breeds used.<br />

However, it is interesting to note that crossbred and PC<br />

postfreezing revivability was higher in T 2 while Murrah<br />

postfreezing revivability responded better in T 3.<br />

Keywords: buffalo spermatozoa, freezing methods,<br />

postfreezing sperm revivability<br />

*Philippine Journal of Veterinary Animal Science. 16( ):76<br />

(Abstr.) .Also in Abstracts of Water Buffalo Researches in<br />

the Philippines. 1981-1995. 1996. PCC-DA. p. 55-56.<br />

Also in Annotated Bibliography on Philippine Biodiversity:<br />

Livestock and Poultry (Agrobiodiversity) 1949-1997,<br />

Carabaos (2002) p. 264.<br />

R-25<br />

<strong>THE</strong> MONTHLY CALVING RATES AND CALVING<br />

INTERVAL OF CARABAOS RAISED IN CONFINE-<br />

MENT AND IN RANCH TYPE OF MANAGEMENT*<br />

V. G. Momongan, Professor, IAS-CA, UPLB<br />

A. S. Dabandan, Undergraduate Student, IAS-CA, UPLB<br />

A. S. Sarabia, Supervising Science Research specialist, PCC-UPLB, DA<br />

F. M. Mamuad, Center Director, PCC-CLSU, DA<br />

71

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