THE PHILIPPINE WATER BUFFALO

B. B. Hufana, Student, DAS-CA, CLSU
D. H. Duran, Science Research Assistant, PCC-CLSU, DA
R. D. Hufana, Research Assistant, PCC-OED, DA
P. G. Duran, Supervising Science Research Specialist, PCC-CLSU, DA
E. P. Garillo, M. S. Student, DAS-IGS, CLSU
L. C. Cruz, Executive Director, PCC-OED, DA
Two studies were conducted to examine the effect of time
on buffalo oocytes in vitro maturation (IVM) and embryo
development after in vitro fertilization (IVF). The general
objective is to come up with a concrete basis for technical
procedure on collection and length of preservation of ovaries
and oocytes IVM for improved rate of in vitro embryo
production. Study 1 examined the effects of short (3-4 hr)
and long (5-6 hr) periods of ovary collection and
preservation on the subsequent development potential of
buffalo oocytes after IVF. A total of 132 oocytes were
randomly distributed into two groups – long period and
short period. Results revealed that cleavage and blastocyct
development rates were higher in the short period than in
the long period, 58.57% vs 46.77% and 21.43% vs
12.9%, respectively (P