THE PHILIPPINE WATER BUFFALO

R-15
ONSET OF ESTRUS, OCCURRENCE OF OVU-
LATION, AND ESTROUS CYCLE LENGTH OF
CYCLIC BUFFALOES AFTER PGF2 ALPHA OR PRID
TREATMENTS*
P. G. Duran, Science Research Specialist, PCRDC-PCARRD, CLSU
H. V. Venturina, Science Research Specialist, PCRDC-PCARRD, CLSU
F. V. Mamuad, PhD Student, IAS-CA, UPLB
L. C. Cruz, Professor, DAS-CA, CLSU and Project Leader, PCRDC-
PCARRD, CLSU
Three studies were conducted to determine the ovarian
response of cyclic water buffaloes to prostaglandin F 2 alpha
(PGF 2 alpha) or progesterone releasing intra-vaginal device
(PRID) treatments. Study 1 compared the effect of PGF 2
alpha and PRID in reference to the onset of estrus and
occurrence of ovulation. Animals in treatment A (14)
received two injections of PGF 2 alpha 11 days apart while
the animals in treatment B (13) had PRID remained in situ
for 11 days. In study II, twelve animals were subjected to
a 2 x 2 factorial experiment to compare the effects of two
types of PGF 2 alpha (Dinoprost tromethamine and
Cloprostenol) and to determine the effect of the stage of the
luteal phase at the time of PGF 2 alpha adminstration on the
variability of the onset of estrus and ovulation. Study II
involved 17 water buffaloes in luteal phase aimed at
determining the ovarian responses immediately after and
in subsequent cycles after consecutive PGF 2 alpha
treatments. Result of the study I indicated no difference in
response to either PGF 2 alpha or PRID on the onset of
estrus and occurrence of ovulation although there was a
tendency for the PRID-treated animals to come to estrus
and ovulate earlier than the other group. Animals treated
with PGF 2 alpha during the early luteal phase (day 7-10)
had the onset of estrus at the same time as those treated
during the late luteal phase (day 15-18). Mean interval
from the treatment to onset of estrus for early and late luteal
phases were 69.8 + 16.6 hours and 68.5 + 19.6 hours,
respectively. The interval of ovulation in the early and late
luteal phase groups did not vary significantly (127.0 + 34.9
hours). In the same respect, responses to two types of
PGF 2 alpha did not differ significantly. Result of the study
II indicated that precocious luteolysis resulting from
consecutive PGF 2 alpha treatments promoted high incidence
of abnormal cycles. Animals with short cycles had an
average length cycle of 7.62 + 2.13 days and represent
52.9 percent of the treated animals. Subsequent cycle
following immediately after short cycle was relatively longer
(15.87 + 5.3 days). Fifty percent of the short cycles
observed immediately following consecutive PGF 2 alpha
treatments where followed by normal cycles whereas the
68
ABSTRACT OF RESEARCHES ON
THE PHILIPPINE WATER BUFFALO
other 50 percent were followed by another short cycle
prior to normal cycle.
Keywords: cyclic water buffaloes, ovarian response,
prostaglandin F 2 alpha, progesterone releasing intra-vaginal
device
*Philippine Journal of Veterinary Animal Science.
16(1&2):72-73 (Abstr.). Also in Annotated Bibliography on
Philippine Biodiversity: Livestock and Poultry
(Agrobiodiversity) 1949-1997, Carabaos (2002) p. 333.
R-16
POST PARTUM REPRODUCTIVE ORGAN ACTIVITY
ON PHILIPPINE CARABAO, MURRAH BUFFALO
AND THEIR CROSSES*
P. G. Duran, Science Research Specialist, PCRDC-PCARRD, CLSU
H. V. Venturina, Science Research Specialist, PCRDC-PCARRD, CLSU
M. Singh, Reproductive Physiologist and FAO Consultant
F. V. Mamuad, Instructor, DAS-CA, CLSU
Observations were made on 15 Philippine caracows (PC),
six Murrah buffalo (MB) and four crossbreds (F 1 ) to study
postpartum changes in the reproductive tract and the
occurrence of first postpartum estrus under ranch
management. Rectal palpations were done 48 to 72 hours
after parturation and at five days interval thereafter, until
complete involution of the cervix and uterus and until the
onset of the next estrus. Results revealed that on the
average, PC, MB and F 1 crossbreds required 38, 39 and
39 days, respectively, to complete cervical and uterine
involution. The mean period postpartum for follicular
development in the PC, MB and F 1 crossbreds were 36,
42 and 21 days, respectively. Average first postpartum
estrus observed for Philippine carabao, Murrah buffalo and
F 1 crossbreds were 94 + 40.05, 107.76 + 15.50 and 76.75
+ 18.59 days, respectively.
Keywords: crossbred carabao, Murrah buffalo, Philippine
caracow, postpartum changes
*Philippine Journal of Veterinary Animal Science.
10(3&4):45 (Abstr.). Also in Abstracts of Water Buffalo
Researches in the Philippines 1981–1995. 1996. PCC-
DA. p. 45-46. Also in Annotated Bibliography on Philippine
Biodiversity: Livestock and Poultry (Agrobiodiversity)
1949-1997, Carabaos (2002) p. 252.
R-17
THE INCIDENCE OF PREGNANT CARABAOS
SLAUGHTERED AT FOOD TERMINAL, INC. FROM
1989-1990*
M. M. Dunwa, Undergraduate Student, CVM, UPLB
C. P. Maala, Professor, CVM, UPLB