THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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Keywords: embryo, in vitro fertilization, in vitro maturation,<br />
oocytes, water buffalo<br />
*Proceedings of the 34 th Annual Convention, Philippines<br />
Society of Animal Science, October 23-24, 1997, PICC.<br />
Manila. p. 304-313. Also in Philippine Journal of Veterinary<br />
Animal Science 1998. 24(1&2):54-64. Also in Annotated<br />
Bibliography on Philippine Biodiversity: Livestock and<br />
Poultry (Agrobiodiversity) 1949-1997, Carabaos. (2002)<br />
p. 395.<br />
R-13<br />
STUDIES ON INCREASING <strong>THE</strong> YIELD OF IN VITRO<br />
EMBRYO PRODUCTION IN <strong>WATER</strong> <strong>BUFFALO</strong>ES*<br />
D. H. Duran, Science Research Assistant II, PCC-CLSU, DA<br />
M. Gunjima, JOCV Volunteer<br />
P. B. Pedro, Senior Agriculturist, PCC-OED, DA<br />
F. P. Aquino, Science Research Assistant, PCC-CLSU, DA<br />
R. de Vera, Laboratory Aide, PCC-OED, DA<br />
P.G. Duran, Supervising Science Research Specialist, PCC-CLSU, DA<br />
L.C. Cruz, Executive Director, PCC-OED, DA<br />
Studies were conducted aimed at increasing the yield of<br />
preimplantation buffalo embryos in vitro. Method for efficient<br />
oocyte retrieval from the ovaries, criteria for selection of<br />
oocytes, and effects of different culture media were<br />
assessed. Results revealed that significantly higher number<br />
of oocytes could be retrieved from the ovaries using the<br />
slicing method than aspiration method (7.33 vs. 3.54<br />
oocytes/ovary, respectively). Similarly, higher number of<br />
good oocytes could be retrieved in using slicing method<br />
than in aspiration method (1.34+0.50 & 1.83+0.25 vs.<br />
0.43+0.15 & 0.92+0.28 Rank A & B oocytes, respectively).<br />
When in vitro development potential of the classified oocytes<br />
were assessed, significantly higher (P