covered with mineral oil and placed in an incubator at 39 0 C with 15% carbon dioxide in air and high humidity. Assessment of nuclear maturation of swamp buffalo oocytes where based on the following: Germinal Vesicle (GV), Germinal Vesicle Breakdown (GVBD), Metaphase I (MI), Telophase I (TI) and Metaphase II (MII) Parthenote and Degenerated. After 24 hours incubation, oocytes were mounted on a glass slide, fixed and cleared with acetic acid: ethanol (1.3v/v) and stained with 1% orcein in 40% acetic acid and was observed under the microscope. The percentage of oocytes that had reached Metaphase II or maturation rates were at 67%, 58%, 59% and 70% for T1, T2, T3 and T4, respectively. Statistical analysis showed that no significant differences exist with respect to its ability to support nuclear maturation. Keywords: immature swamp buffalo oocytes, IVM, maturation media, nuclear maturation *Undergraduate Thesis, College of Veterinary Medicine, CLSU, Muñoz, Nueva Ecija (1997). R-7 BLOOD PLASMA PROGESTERONE IN NORMALLY ESTROUS CYCLING AND ANESTROUS <strong>PHILIPPINE</strong> CARABAO HEIFERS* L. N. Chua, M.S. Student, IAS-CA, UPLB A. S. Sarabia, Science Research Specialist, PCRDC-PCARRD, UPLB N. P. Roxas, Assistant Professor, IAS-CA, UPLB Z. M. Nava, Science Research Specialist, PCRDC-PCARRD, UPLB V. G. Momongan, Prof., IAS-CA, UPLB & Proj. Leader, PCRDC-PCARRD, UPLB Regularly cycling carabao heifers had plasma progesterone level which was lowest at estrus (0.241 + 0.134 ng/ml) and which gradually increased to reach a peak (1.759 + 0.187 ng/ ml) at luteal phase, about day 12 to 14, and then gradually decline thereafter until it reached the lowest level at the next estrus. On the other hand, anestrus caraheifers had two patterns of plasma progesterone levels. One group had similar plasma progesterone pattern as the regular cycling animals, the other group had no cycling pattern but had plasma progesterone levels which remained low (0.164 to 0.718 ng/ml) throughout the expected length of the estrous cycle. Keywords: anestrus, blood plasma progesterone, carabao heifers, estrus *Abstract of Completed Researches 1981-1995 (1985). PCC- DA, UPLB, College, Laguna. Also in Abstracts of Water Buffalo researches in the Philippines 1981-1995. 1996. PCC- DA. p. 39. Also in Philippine Journal of Veterinary Animal Science, 10(1&2):41 (Abstr.). Also in Annotated Bibliography on Philippine Biodiversity: Livestock and Poultry (Agrobiodiversity) 1949-1997, Carabaos. (2002) p. 325. 64 ABSTRACT OF RESEARCHES ON <strong>THE</strong> <strong>PHILIPPINE</strong> <strong>WATER</strong> <strong>BUFFALO</strong> R-8 GROSS PATHOLOGICAL DISORDERS OF <strong>THE</strong> INTERNAL REPRODUCTIVE ORGANS OF SLAUGHTERED <strong>PHILIPPINE</strong> <strong>BUFFALO</strong>-COWS (Bubalus bubaline) IN RELATION TO INFERTILITY PROBLEMS* S. H. Corcoro, Student, CVM, UP, Diliman, Quezon City The gross pathological disorders of the internal reproductive organs of the slaughtered Philippine buffalo-cows is herein presented. The gross pathological disorders observed and their incidence are: follicular cyst, 8.33 percent; cystic corpera lutea, 5.30 percent; parovarian cyst, 7.30 percent, ovarian hematoma, 0.49 percent; adhesions, 4.9 percent; hydrosalpinx, 5.88 percent, mucometra, 3.40 percent; pyometra, 0.98 percent; hypoplasia 4.9, percent; and cystic glandular hypoplasia, 5.3 percent; cervicitis, 5.9 percent; vaginitis, 2.90 percent; cervical cyst, 1.96 percent; and tumor, 2.45 percent. Of the gross pathological disorders observed, follicular cyst, hydrosalpinx, mucometra, pyometra and hypoplasia will cause infertility in the affected animal. Generalized adhesions especially if it involves the ovaries, will also result to infertility. Cystic glandular hyperplasia and uterine tumor if extensive, may order the endometrium inhospitable to ovum implantation. Cervicitis and vaginitis may cause infertility if the condition extends to the uterus. Cervical cysts may obstruct the cervix and prevent insemination or it may destroy the cervical plug of pregnancy facilitating uterine infection. Parovarian cysts do not affect the fertility of the animal and cystic corpora lutea are known to occur in infertile animals. Further studies should be conducted to determine if the gross pathological disorders observed in the present study also occur in nonslaughter animals. Keywords: Philippine carabao cows, pathological disorders, internal reproductive organs, infertility problems *Undergraduate Thesis, College of Veterinary Medicine, University of the Philippines, Diliman, Quezon City (1978). R-9 OVARIAN RESPONSES AND PREGNANCY RATE OF CARABAOS TREATED WITH PGF 2 ALPHA FOLLOWED BY GnRH AT PREDETERMINED TIME* L. C. Cruz, Professor, DAS-CA, CLSU & Project Leader, PCRDC- PCARRD, CLSU P. G. Duran, Science Research Specialist; PCRDC-PCARRD, CLSU H. V. Venturina, Science Research Specialist; PCRDC-PCARRD, CLSU E. Aquino, Science Research Assistant, PCRDC-PCARRD, CLSU D. Hufana, Science Research Assistant, PCRDC-PCARRD, CLSU F. Rellin, M. Student, IGS, CLSU O. F. Smith, PhD Student, IGS, CLSU
Series of experiment were conducted aimed at developing least-cost and more effective synchronization scheme for the carabaos in the villages. Experiment I focused on the understanding of the occurrence of estrus and ovulation in carabaos after prostaglandin F 2 alpha (PGF 2 alpha) treatment. Result obtained from 36 cycling caracows administered with two PGF 2 alpha injections (1 mg fenprostalene, i.m./injection) 11 days apart indicated that the onset of estrus was 60.8 + 13 hours. After the last PGF 2 alpha treatment and estrus duration was 20.0 + 3.1 hours. Ovulation occurred 91.6 + 9.5 hours after the last PGF 2 alpha treatment. In Experiment II, 28 cycling caracows were assigned to 2 x 3 factorial experiment involving two PGF 2 alpha preparations (fenprostalene and dinoprost tromethamine) and three intervals of gonadotrophin releasing hormone (GnRH) treatment after PGF 2 alpha (60, 72 and 84 hours) to identify the more appropriate PGF 2 alpha estrus synchronization and the proper time of GnRH treatment for induction of ovulation. All animals received two injections of PGF 2 alpha (1 mg fenprostalene or 25 mg dinoprost tromethamine/injection). GnRH (100 ug fertilerin acetate, i.m.) was given to respective treatment groups at either 60, 72 or 84 hours after the last PGF 2 alpha administration. There was no difference in the estrus response of the animals after the GnRH treatement. However, the incidence of abnormal luteal phases was significantly higher (P
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ABSTRACT OF RESEARCHES ON THE PHILI
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USER’S GUIDE The book contains pu
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ACKNOWLEDGMENT Grateful recognition
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CONTENTS Inoc, J. G. and C. P. Maal
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CONTENTS Viernes, Jr., V. D. 2004.
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CONTENTS Harada, H., et al. Ultraso
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CONTENTS Ocampo, M. B., et al. Clea
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CONTENTS Aranas, T. J., et al. 1968
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CONTENTS Romulo, B. H., et al. Dige
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ANATOMY A-1 DISTRIBUTION AND MICROS
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of the islet. The pancreatic- polyp
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(P>0.05) from that of the Philippin
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A-15 THE MODIFIED HORN RING METHOD
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undifferentiated cells. Mucigenous
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*Abstracts of Completed Researches,
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70.97 mg percent. Age and sex seeme
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levels (8.3 mg/100 ml vs. 11.0 mg/1
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for the first 7 months. Tethering p
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L. S. Castillo, Professor, Departme
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Keywords: caraheifers, chromic oxid
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similar. Addition of 1% urea in the
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espectively. Detergent fiber analys
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V. S. Subere, Former M. S. Student,
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serovars were used namely: Leptospi
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was conducted in the abattoirs of M
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Pseudomonas sp. (2.86%), Pasteurell
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*Undergraduate thesis, College of V
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upper respiratory tract of normal c
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studies are necessary to find possi
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permanent cheek teeth, 26 permanent
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uy back operation, 11 calving inter
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caraheifers, 2, 27, 45 carcass qual