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THE PHILIPPINE WATER BUFFALO

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Series of experiment were conducted aimed at developing<br />

least-cost and more effective synchronization scheme for<br />

the carabaos in the villages. Experiment I focused on the<br />

understanding of the occurrence of estrus and ovulation in<br />

carabaos after prostaglandin F 2 alpha (PGF 2 alpha)<br />

treatment. Result obtained from 36 cycling caracows<br />

administered with two PGF 2 alpha injections (1 mg<br />

fenprostalene, i.m./injection) 11 days apart indicated that<br />

the onset of estrus was 60.8 + 13 hours. After the last<br />

PGF 2 alpha treatment and estrus duration was 20.0 + 3.1<br />

hours. Ovulation occurred 91.6 + 9.5 hours after the last<br />

PGF 2 alpha treatment. In Experiment II, 28 cycling<br />

caracows were assigned to 2 x 3 factorial experiment<br />

involving two PGF 2 alpha preparations (fenprostalene and<br />

dinoprost tromethamine) and three intervals of<br />

gonadotrophin releasing hormone (GnRH) treatment after<br />

PGF 2 alpha (60, 72 and 84 hours) to identify the more<br />

appropriate PGF 2 alpha estrus synchronization and the<br />

proper time of GnRH treatment for induction of ovulation.<br />

All animals received two injections of PGF 2 alpha (1 mg<br />

fenprostalene or 25 mg dinoprost tromethamine/injection).<br />

GnRH (100 ug fertilerin acetate, i.m.) was given to<br />

respective treatment groups at either 60, 72 or 84 hours<br />

after the last PGF 2 alpha administration. There was no<br />

difference in the estrus response of the animals after the<br />

GnRH treatement. However, the incidence of abnormal<br />

luteal phases was significantly higher (P

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