THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
covered with mineral oil and placed in an incubator at 39 0 C<br />
with 15% carbon dioxide in air and high humidity.<br />
Assessment of nuclear maturation of swamp buffalo oocytes<br />
where based on the following: Germinal Vesicle (GV),<br />
Germinal Vesicle Breakdown (GVBD), Metaphase I (MI),<br />
Telophase I (TI) and Metaphase II (MII) Parthenote and<br />
Degenerated. After 24 hours incubation, oocytes were<br />
mounted on a glass slide, fixed and cleared with acetic<br />
acid: ethanol (1.3v/v) and stained with 1% orcein in 40%<br />
acetic acid and was observed under the microscope. The<br />
percentage of oocytes that had reached Metaphase II or<br />
maturation rates were at 67%, 58%, 59% and 70% for<br />
T1, T2, T3 and T4, respectively. Statistical analysis showed<br />
that no significant differences exist with respect to its ability<br />
to support nuclear maturation.<br />
Keywords: immature swamp buffalo oocytes, IVM,<br />
maturation media, nuclear maturation<br />
*Undergraduate Thesis, College of Veterinary Medicine,<br />
CLSU, Muñoz, Nueva Ecija (1997).<br />
R-7<br />
BLOOD PLASMA PROGESTERONE IN NORMALLY<br />
ESTROUS CYCLING AND ANESTROUS <strong>PHILIPPINE</strong><br />
CARABAO HEIFERS*<br />
L. N. Chua, M.S. Student, IAS-CA, UPLB<br />
A. S. Sarabia, Science Research Specialist, PCRDC-PCARRD, UPLB<br />
N. P. Roxas, Assistant Professor, IAS-CA, UPLB<br />
Z. M. Nava, Science Research Specialist, PCRDC-PCARRD, UPLB<br />
V. G. Momongan, Prof., IAS-CA, UPLB & Proj. Leader, PCRDC-PCARRD, UPLB<br />
Regularly cycling carabao heifers had plasma progesterone<br />
level which was lowest at estrus (0.241 + 0.134 ng/ml) and<br />
which gradually increased to reach a peak (1.759 + 0.187 ng/<br />
ml) at luteal phase, about day 12 to 14, and then gradually<br />
decline thereafter until it reached the lowest level at the next<br />
estrus. On the other hand, anestrus caraheifers had two patterns<br />
of plasma progesterone levels. One group had similar plasma<br />
progesterone pattern as the regular cycling animals, the other<br />
group had no cycling pattern but had plasma progesterone<br />
levels which remained low (0.164 to 0.718 ng/ml) throughout<br />
the expected length of the estrous cycle.<br />
Keywords: anestrus, blood plasma progesterone, carabao<br />
heifers, estrus<br />
*Abstract of Completed Researches 1981-1995 (1985). PCC-<br />
DA, UPLB, College, Laguna. Also in Abstracts of Water<br />
Buffalo researches in the Philippines 1981-1995. 1996. PCC-<br />
DA. p. 39. Also in Philippine Journal of Veterinary Animal<br />
Science, 10(1&2):41 (Abstr.). Also in Annotated Bibliography<br />
on Philippine Biodiversity: Livestock and Poultry<br />
(Agrobiodiversity) 1949-1997, Carabaos. (2002) p. 325.<br />
64<br />
ABSTRACT OF RESEARCHES ON<br />
<strong>THE</strong> <strong>PHILIPPINE</strong> <strong>WATER</strong> <strong>BUFFALO</strong><br />
R-8<br />
GROSS PATHOLOGICAL DISORDERS OF <strong>THE</strong><br />
INTERNAL REPRODUCTIVE ORGANS OF<br />
SLAUGHTERED <strong>PHILIPPINE</strong> <strong>BUFFALO</strong>-COWS<br />
(Bubalus bubaline) IN RELATION TO<br />
INFERTILITY PROBLEMS*<br />
S. H. Corcoro, Student, CVM, UP, Diliman, Quezon City<br />
The gross pathological disorders of the internal reproductive<br />
organs of the slaughtered Philippine buffalo-cows is herein<br />
presented. The gross pathological disorders observed and<br />
their incidence are: follicular cyst, 8.33 percent; cystic<br />
corpera lutea, 5.30 percent; parovarian cyst, 7.30 percent,<br />
ovarian hematoma, 0.49 percent; adhesions, 4.9 percent;<br />
hydrosalpinx, 5.88 percent, mucometra, 3.40 percent;<br />
pyometra, 0.98 percent; hypoplasia 4.9, percent; and cystic<br />
glandular hypoplasia, 5.3 percent; cervicitis, 5.9 percent;<br />
vaginitis, 2.90 percent; cervical cyst, 1.96 percent; and<br />
tumor, 2.45 percent. Of the gross pathological disorders<br />
observed, follicular cyst, hydrosalpinx, mucometra,<br />
pyometra and hypoplasia will cause infertility in the affected<br />
animal. Generalized adhesions especially if it involves<br />
the ovaries, will also result to infertility. Cystic glandular<br />
hyperplasia and uterine tumor if extensive, may order the<br />
endometrium inhospitable to ovum implantation. Cervicitis<br />
and vaginitis may cause infertility if the condition extends<br />
to the uterus. Cervical cysts may obstruct the cervix and<br />
prevent insemination or it may destroy the cervical plug of<br />
pregnancy facilitating uterine infection. Parovarian cysts<br />
do not affect the fertility of the animal and cystic corpora<br />
lutea are known to occur in infertile animals. Further studies<br />
should be conducted to determine if the gross pathological<br />
disorders observed in the present study also occur in nonslaughter<br />
animals.<br />
Keywords: Philippine carabao cows, pathological<br />
disorders, internal reproductive organs, infertility problems<br />
*Undergraduate Thesis, College of Veterinary Medicine,<br />
University of the Philippines, Diliman, Quezon City (1978).<br />
R-9<br />
OVARIAN RESPONSES AND PREGNANCY RATE OF<br />
CARABAOS TREATED WITH PGF 2 ALPHA<br />
FOLLOWED BY GnRH AT PREDETERMINED TIME*<br />
L. C. Cruz, Professor, DAS-CA, CLSU & Project Leader, PCRDC-<br />
PCARRD, CLSU<br />
P. G. Duran, Science Research Specialist; PCRDC-PCARRD, CLSU<br />
H. V. Venturina, Science Research Specialist; PCRDC-PCARRD, CLSU<br />
E. Aquino, Science Research Assistant, PCRDC-PCARRD, CLSU<br />
D. Hufana, Science Research Assistant, PCRDC-PCARRD, CLSU<br />
F. Rellin, M. Student, IGS, CLSU<br />
O. F. Smith, PhD Student, IGS, CLSU