THE PHILIPPINE WATER BUFFALO

covered with mineral oil and placed in an incubator at 39 0 C
with 15% carbon dioxide in air and high humidity.
Assessment of nuclear maturation of swamp buffalo oocytes
where based on the following: Germinal Vesicle (GV),
Germinal Vesicle Breakdown (GVBD), Metaphase I (MI),
Telophase I (TI) and Metaphase II (MII) Parthenote and
Degenerated. After 24 hours incubation, oocytes were
mounted on a glass slide, fixed and cleared with acetic
acid: ethanol (1.3v/v) and stained with 1% orcein in 40%
acetic acid and was observed under the microscope. The
percentage of oocytes that had reached Metaphase II or
maturation rates were at 67%, 58%, 59% and 70% for
T1, T2, T3 and T4, respectively. Statistical analysis showed
that no significant differences exist with respect to its ability
to support nuclear maturation.
Keywords: immature swamp buffalo oocytes, IVM,
maturation media, nuclear maturation
*Undergraduate Thesis, College of Veterinary Medicine,
CLSU, Muñoz, Nueva Ecija (1997).
R-7
BLOOD PLASMA PROGESTERONE IN NORMALLY
ESTROUS CYCLING AND ANESTROUS PHILIPPINE
CARABAO HEIFERS*
L. N. Chua, M.S. Student, IAS-CA, UPLB
A. S. Sarabia, Science Research Specialist, PCRDC-PCARRD, UPLB
N. P. Roxas, Assistant Professor, IAS-CA, UPLB
Z. M. Nava, Science Research Specialist, PCRDC-PCARRD, UPLB
V. G. Momongan, Prof., IAS-CA, UPLB & Proj. Leader, PCRDC-PCARRD, UPLB
Regularly cycling carabao heifers had plasma progesterone
level which was lowest at estrus (0.241 + 0.134 ng/ml) and
which gradually increased to reach a peak (1.759 + 0.187 ng/
ml) at luteal phase, about day 12 to 14, and then gradually
decline thereafter until it reached the lowest level at the next
estrus. On the other hand, anestrus caraheifers had two patterns
of plasma progesterone levels. One group had similar plasma
progesterone pattern as the regular cycling animals, the other
group had no cycling pattern but had plasma progesterone
levels which remained low (0.164 to 0.718 ng/ml) throughout
the expected length of the estrous cycle.
Keywords: anestrus, blood plasma progesterone, carabao
heifers, estrus
*Abstract of Completed Researches 1981-1995 (1985). PCC-
DA, UPLB, College, Laguna. Also in Abstracts of Water
Buffalo researches in the Philippines 1981-1995. 1996. PCC-
DA. p. 39. Also in Philippine Journal of Veterinary Animal
Science, 10(1&2):41 (Abstr.). Also in Annotated Bibliography
on Philippine Biodiversity: Livestock and Poultry
(Agrobiodiversity) 1949-1997, Carabaos. (2002) p. 325.
64
ABSTRACT OF RESEARCHES ON
THE PHILIPPINE WATER BUFFALO
R-8
GROSS PATHOLOGICAL DISORDERS OF THE
INTERNAL REPRODUCTIVE ORGANS OF
SLAUGHTERED PHILIPPINE BUFFALO-COWS
(Bubalus bubaline) IN RELATION TO
INFERTILITY PROBLEMS*
S. H. Corcoro, Student, CVM, UP, Diliman, Quezon City
The gross pathological disorders of the internal reproductive
organs of the slaughtered Philippine buffalo-cows is herein
presented. The gross pathological disorders observed and
their incidence are: follicular cyst, 8.33 percent; cystic
corpera lutea, 5.30 percent; parovarian cyst, 7.30 percent,
ovarian hematoma, 0.49 percent; adhesions, 4.9 percent;
hydrosalpinx, 5.88 percent, mucometra, 3.40 percent;
pyometra, 0.98 percent; hypoplasia 4.9, percent; and cystic
glandular hypoplasia, 5.3 percent; cervicitis, 5.9 percent;
vaginitis, 2.90 percent; cervical cyst, 1.96 percent; and
tumor, 2.45 percent. Of the gross pathological disorders
observed, follicular cyst, hydrosalpinx, mucometra,
pyometra and hypoplasia will cause infertility in the affected
animal. Generalized adhesions especially if it involves
the ovaries, will also result to infertility. Cystic glandular
hyperplasia and uterine tumor if extensive, may order the
endometrium inhospitable to ovum implantation. Cervicitis
and vaginitis may cause infertility if the condition extends
to the uterus. Cervical cysts may obstruct the cervix and
prevent insemination or it may destroy the cervical plug of
pregnancy facilitating uterine infection. Parovarian cysts
do not affect the fertility of the animal and cystic corpora
lutea are known to occur in infertile animals. Further studies
should be conducted to determine if the gross pathological
disorders observed in the present study also occur in nonslaughter
animals.
Keywords: Philippine carabao cows, pathological
disorders, internal reproductive organs, infertility problems
*Undergraduate Thesis, College of Veterinary Medicine,
University of the Philippines, Diliman, Quezon City (1978).
R-9
OVARIAN RESPONSES AND PREGNANCY RATE OF
CARABAOS TREATED WITH PGF 2 ALPHA
FOLLOWED BY GnRH AT PREDETERMINED TIME*
L. C. Cruz, Professor, DAS-CA, CLSU & Project Leader, PCRDC-
PCARRD, CLSU
P. G. Duran, Science Research Specialist; PCRDC-PCARRD, CLSU
H. V. Venturina, Science Research Specialist; PCRDC-PCARRD, CLSU
E. Aquino, Science Research Assistant, PCRDC-PCARRD, CLSU
D. Hufana, Science Research Assistant, PCRDC-PCARRD, CLSU
F. Rellin, M. Student, IGS, CLSU
O. F. Smith, PhD Student, IGS, CLSU