THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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REPRODUCTION<br />
R-1<br />
RADIOIMMUNOASSAY OF LH IN <strong>THE</strong> CARABAO*<br />
A. Alejandrino, Researcher, PAEC<br />
J. Alcantara, Researcher, PAEC<br />
E. Wagelie, Researcher, NABC-DA and BAI-DA (Alabang)<br />
C. Goze, Researcher, NABC-DA and BAI-DA (Alabang)<br />
J. Sevilla, Researcher, NABC-DA and BAI-DA (Alabang)<br />
A. Caburian, Researcher, NABC-DA and BAI-DA (Alabang)<br />
B. Oprenario, Researcher, NABC-DA and BAI-DA (Alabang)<br />
A sensitive method for the measurement of leutenizing<br />
hormone (LH) in carabao serum using radioimmunoassay<br />
was validated. To better understand carabao reproductive<br />
physiology and to evolve to breeding management scheme<br />
to improve reproductive efficiency, LH concentrations with<br />
focus on the LH surge event in 21 adult female carabaos<br />
managed under confinement, were determined. The majority<br />
of the animals (57.1%) showed the preovulatory LH peak<br />
values (5mg/ml or greater); others gave medium or low<br />
peaks. Basal, tonic values (0.4 + 0.2 mg/ml) were<br />
observed during the estrous cycle except for the brief LH<br />
surge which appeared either at early or late estrus stage.<br />
During pregnancy, LH concentration also remained basal<br />
(0.5 + 0.3 ng/ml) apparently suppressed by the sustained<br />
higher levels of progesterone.<br />
Keywords: carabao serum, radioimmunoassay of LH<br />
*Philippine Journal of Veterinary Animal Science,<br />
10(3&4):40-41 (Abstr.). Also in Annotated Bibliography on<br />
Philippine Biodiversity: Livestock and Poultry<br />
(Agrobiodiversity) 1949-1997, Carabaos, (2002), p. 324.<br />
R-2<br />
EFFECT OF TIME OF GnRH ADMINISTRATION ON<br />
OVULATION RESPONSE AND CORPUS LUTEUM<br />
FUNCTION IN POSTPARTUM <strong>BUFFALO</strong> COWS<br />
TREATED WITH PGF 2 ALPHA FOR<br />
SYNCHRONIZATION*<br />
E. J. Aquino, M. S. Student, IGS, CLSU<br />
P. G. Duran, Science Research Specialist, PCRDC-PCARRD, CLSU<br />
H.V. Venturina, Science Research Specialist, PCRDC-PCARRD, CLSU<br />
F. Rellin, M. S. Student, IGS, CLSU<br />
O. F. Smith, PhD Student, IGS, CLSU<br />
L. C. Cruz, Prof., DAS-CA, CLSU and Project Leader, PCRDC-PCARRD, CLSU<br />
Twenty-eight (28) cyclic caracows were assigned to a 2 x<br />
3 factorial experiment involving two-prostaglandin F 2 alpha<br />
(PGF 2 alpha) (fenprostalene and dinoprost tromethamine)<br />
preparations and three intervals of GnRH treatment after<br />
PGF 2 alpha (60, 72 and 84 hours) injection. The study<br />
62<br />
ABSTRACT OF RESEARCHES ON<br />
<strong>THE</strong> <strong>PHILIPPINE</strong> <strong>WATER</strong> <strong>BUFFALO</strong><br />
aimed to identify the appropriate type of PGF 2 alpha for<br />
estrus synchronization and the proper time of gonadotropin<br />
releasing hormone (GnRH) treatment for induction of<br />
ovulation. All animals were injected twice with PGF 2<br />
alpha (1 mg fenprostalene or 25 mg dinoprost tromethamine/<br />
injection). The same animals were given GnRH (100 ug<br />
fertilerin acetate, IM) 60, 72 or 84 hours after the last PGF 2<br />
alpha administration. Results showed no significant<br />
differences in the estrus responses of the animals to the<br />
two types of PGF 2 alpha. All the animals ovulated after<br />
GnRH treatment. However, the incidence of abnormal<br />
luteal phases was significantly higher (P
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