THE PHILIPPINE WATER BUFFALO

stock farms which practiced backyard-type production,
e.g. La Carlota. Results of the analysis with reference to
main effects and two-factor interactions confirmed most of
the previous statistical analyses. The present analysis is
confined more on comparative performances in relation to
locations and type of farming system (feeding and
management).
Keywords: backyard and ranch-type production, carabao
and crossbreds, growth performance, institutional herds
*Abstracts of Completed Researches, 1981-1995, (1996)
PCC-DA, UPLB. Also in Abstracts of Water Buffalo
Researches in the Philippines, 1981-1995, (1996) PCC-
DA p. 4-5. Also in Annotated Bibliography on Philippine
Biodiversity: Livestock and Poultry (Agrobiodiversity)
1949-1997, Carabaos (2002) p. 243
B-31
RAPD ANALYSIS OF INDIAN MURRAH WATER
BUFFALO, NATIVE CARABAO AND THEIR F 1 *
B. P. Roxas, Graduate Student, Molecular Biology and Biotech Program,
UPDiliman
S. C. Halos, Professor, Molecular Biology and Biotechnology Program,
UPDiliman
A. S. Sarabia, Supervising Science Research Specialist, PCC-DA, UPLB
RAPD assay was used to identify polymorphic DNA
markers between Murrah buffalo, native carabao and their
F1. Genomic DNA was extracted from blood samples.
PCR amplification using six UBC primers was done using
25 ng DNA template and 2.5 mM Mg 2 Cl in a 25 ul reaction
mixture under the following conditions: single cycle of 5
mins at 95 o C, 45 cycles of 5 sec at 94 o C, 1 min at 34 o C,
2 mins at 72 o C and final elongation of 5 mins. PCR
amplification products were resolved in 5% polyacrylamide
gel. Four primers generated one to nine bands ranging
from 175 to 2150 bp in length. Nei’s similarity coefficient
was calculated to be S XY = 0.706 between Murrah and
native carabao. The F 1 was observed to exhibit Mendelian
inheritance of bands as well as the generations of nonparental
bands.
Keywords: RAPD assay, DNA markers, water buffalo,
native carabao, F 1 cross
*Proceedings of the 34 th Annual Convention, Philippine
Society of Animal Science, October 23-24, 1997, PICC,
Manila, Philippines. p. 314-319. Also in Completed and
On-going Research Studies, PCC at UPLB, January 1-
December 31, 1997. p. 1-6. Also in Annotated Bibliography
on Philippine Biodiversity: Livestock and Poultry
(Agrobiodiversity) 1949-1997, Carabaos (2002) p. 304
BREEDING AND GENETICS
B-32
THE DETERMINATION OF LIVE WEIGHT AND
CARCASS WEIGHT BASED ON BODY
MEASUREMENT OF PHILIPPINE CARABAOS*
R. Saladin, M. S. Student, DAS-CA, UPLB
B. A. Parker, Associate Professor, DAS-CA, UPLB
Five hundred and thirty two Philippine native carabaos
were grouped according to sex (males, females and steers),
age (below five years old over five years old) and sources
(Luzon region , Visayas region and Mindanao region).
Measurements of body length and heart girth revealed
consistent correlation with liveweight and carcass weight
in the carabaos used. The average correlation coefficient
(r) of live weight with body length and heart girth or animal
from Luzon are 0.84 and 0.912, respectively. For animals
from Visayas region, these estimates are 0.751 and 0.894,
respectively, and for animals from Mindanao region 0.785
and 0.905, respectively; all being statistically significant
(P