THE PHILIPPINE WATER BUFFALO

different in trms of shear force values. However, carabeef
from PM F 1 had significantly lowere penetrometer reading
than that of PC. Carabeef from PM F 1 did not differ
significantly from that of PC of PM F 2 in terms of
penetrometer reading. Mean sensory scores of carabeef
samples from PC and Phil-Murrah crossbreeds were found
to be similar except for tenderness. The carabeef samples
had similar acceptability scores. The results of the study
suggest that crossbreeding scheme did not significantly
affect the muscle fiber size, shear force values and most of
the sensory traits of carabeef.
Keywords: Philippine carabao, crossbreeds, carabeef,
muscle fiber, sensory characteristics
*Philippine Journal of Veterinary Animal Science, 22
(3&4):17-23.
B-26
BLOOD TRANSFERRIN TYPES OF PHILIPPINE
CARABAOS*
B. A. Parker, Assistant Professor, DAH-CA, UPLB
V. G. Momongan, Assistant Professor, DAH-CA, UPLB
The existence of polymorphic plasma trasferrin in Philippine
carabao has been ascertained by means of disc and starch
gel electrophoresis. Three electrophoretic patterns,
Phenotype I, II and III, in blood samples showed
Phenotype I, which is characterized by two distinct and
densely stained brands with a very faint leading band.
Differences in the degree of stain absorption, size of bands
and rate of migration were noted between the cattle and the
carabao transferrin bands. The possible applications of
these findings are discussed.
Keywords: blood transferrin types, frequency distribution,
Phil. carabao
*Philippine Journal of Veterinary Animal Science, 8(2):
87–92. Also in Annotated Bibliography on Philippine
Biodiversity: Livestock and Poultry (Agrobiodiversity)
1949-1997, Carabaos (2002) p. 314.
B-27
THE DISTRIBUTION OF AMYLASE AND CARBONIC
ANHYDRASE GENOTYPES IN PHILIPPINE CATTLE
AND CARABAO*
B. A. Parker, Associate Professor, DAS-CA, UPLB
V. G. Momongan, Associate Professor, DAS-CA, UPLB
P. S. Sotaridona, Research Assistant, DAS-CA, UPLB
A modified technique of horizontal starch gel electrophoresis
in a discontinuous buffer system was used for esterase
BREEDING AND GENETICS
determination in blood samples from 293 mature Philippine
cattle and 118 carabaos. The analysis indicated the
presence of esterase polymorphs in the blood of Philippine
cattle and carabaos. Inhibition test was performed with the
use of 10-4M EDTA, a specific inhibitor for esterase, in
order to confirm that the investigation is esterase. There
are no phenotypes of esterase (ES to the A and ES to the
AB) observed in cattle and only one phenotype (ES to the
A) in carabaos. The ES to the A was common to both
cattle and carabao and ES to the AB was absent in carabao.
The ES to the AB was found in only six cattle.
Keywords: amylase and carbonic anhydrase genotypes,
Philippine carabao and cattle, starch gel electrophoresis
*Abstract of Completed Researches 1973-1995. IAS-CA,
UPLB, College, Laguna (1996). Compiled by A. T. Banzon,
N. L. de Castro, S. M. Ramos, F. F. Peñalba and D. B.
Roxas. 1996. p. 11-12. Also in Annotated Bibliography on
Philippine Biodiversity: Livestock and Poultry
(Agrobiodiversity) 1949-1997, Carabaos. 2002, p. 315
B-28
THE DISTRIBUTION OF ESTERASE, ACID
PHOSPHATASE AND ALKALINE PHOSPHATASE
GENOTYPES IN PHILIPPINE CATTLE AND
CARABAO*
B. A. Parker, Associate Professor, IAS-CA, UPLB
V. G. Momongan, Associate Professor, IAS-CA, UPLB
P. S. Sotaridona, Research Assistant, IAS-CA, UPLB
Esterase and alkaline phosphatase polymorphisms were
determined in hemolysates and the plasma using the starchgel
electrophoresis system. Electrophoretic results indicated
that long storage of the sample affects the zone pattern of the
enzyme as exhibited by lighter intensity and diffused bands
which were difficult to identify. The results also showed that
esterase and alkaline phosphatase activities are inhibited by
10 to the – 4M solution of ethylenediamine tetra-acetic acid
and 5 x 10 to the -3M solution of L-phenylalamine, respectively.
This led to the disappearance of the bands which confirmed
the activity of the enzymes being investigated. Base on the
limited number of samples, two phenotypes of Esterase
(ES to the A and ES to the AB) were observed in cattle and
one phenotype (ES to the A) in carabaos. Two phenotypes
of alkaline phosphates (Alp to the A) and Apl to the AO) were
observed to be common in both cattle and carabao.
Phenotype A of both enzymes migrated ahead of phenotypes
B and O.
Keywords: esterase and alkaline phosphatase,
polymorphisms, Philippine cattle and carabao, starch-gel
electrophoresis
57