THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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P. Nabheerong, Ph.D. Student, IAS-CA, UPLB<br />
V. G. Momongan, Professor, IAS-CA, UPLB & Proj. Leader, PCRDC-<br />
PCARRD, UPLB<br />
Four bulls of Philippines carabao, Murrah buffalo and Phil-<br />
Murrah (Philippines carabao x Murrah buffalo); and storage<br />
times of one week, one month, three month, six month,<br />
nine month and 12 month for frozen semen were analyzed<br />
using a split-plot in Completely Randomized Design (CRD).<br />
The initial quality of the semen ejaculates with respect to<br />
semen volume, sperm concentration, percentage motility,<br />
dead-alive staining and morphology were evaluated. The<br />
semen ejaculates were diluted with Tris-glycerol extender<br />
and processed for storage in liquid nitrogen. Criteria for<br />
examination of frozen semen were percent motility, deadalive<br />
staining and morphological changes of sperm cell<br />
organelles as seen under the electron microscope. One<br />
year of storage of buffalo semen showed no effect on<br />
percent motility, dead-alive staining and morphological<br />
change of sperm cell organelles. The lowest percentage of<br />
abnormal sperm and abnormal acrosome was obtained<br />
from crossbred.<br />
Keywords: buffalo breedtype, frozen semen, semen<br />
quality, stored semen<br />
*Abstracts of Completed Researches, 1981-1995, PCC-<br />
UPLB, College, Laguna. Also in Abstracts of Water<br />
Buffalo Researches in the Philippines 1981-1995 (1996)<br />
PCC-DA, p. 60. Also in Annotated Bibliography on<br />
Philippine Biodiversity: Livestock and Poultry<br />
(Agrobiodiversity), 1949-1997, Carabaos (2002) p. 296.<br />
B-24<br />
TIMING OF SEQUENTIAL CHANGES ON<br />
CHROMOSOME CONFIGURATIONS DURING <strong>THE</strong><br />
1 ST , MEIOSIS, 2 ND MEIOSIS AND CYTOPLASMIC<br />
EVENTS OF SWAMP <strong>BUFFALO</strong> OOCYTES<br />
MATURED AND FERTILIZED IN VITRO*<br />
L. C. Ocampo, Senior Science Research Specialist, OED-PCC-DA<br />
M. B. Ocampo, Supervising Science Research Specialist, OED-PCC-DA<br />
F. V. Mamuad, Associate Professor, DAS-CA, CLSU<br />
E. V. Venturina, Science Research Specialist, PCC-DA, CLSU<br />
T. Mori, Faculty of Agriculture, Hokkaido University<br />
H. Shimizu, Faculty of Agriculture, Hokkaido University<br />
Swamp buffalo oocytes were examined to determine the<br />
specifics of early events of maturation and fertilization in<br />
vitro. A variation in germinal vesicle morphology revealed<br />
an asynchronous oocyte population at the time of collection<br />
(o hr culture). Germinal vesicle breakdown was noticed<br />
from 8-11.7 hr of culture, Metaphase I stage at 11.7 – 19.3<br />
hr. Anaphase I and Telophase I stages were seen<br />
occasionally between 19.3–21 hr and 21.1- 22.2 hr,<br />
56<br />
ABSTRACT OF RESEARCHES ON<br />
<strong>THE</strong> <strong>PHILIPPINE</strong> <strong>WATER</strong> <strong>BUFFALO</strong><br />
respectively, while Metaphase II stage was prominent<br />
between 22.2 – 24.0 hr of culture. The first evidence of<br />
sperm penetration was observed 1 hr after insemination.<br />
Attachement of spermatozoa in the ooplasm resulted to the<br />
initiation of 2 nd meiosis and was seen from 5 – 8 hr post<br />
insemination. Further decondensation of penetrating sperm<br />
head in association with female chromosome<br />
decondensation, accompanied with nuclear envelope<br />
formation was noted between 8–11 hr post insemination.<br />
Opposing and apposing pronuclei were frequently seen<br />
between 11–23 hr after insemination. Syngamy, prophase<br />
stage of 1 st mitotic division, including first cleavage, were<br />
observed as early as 20 hr after insemination.<br />
Keywords: asynchrony, buffalo, meiosis, oocyte,<br />
syngamy<br />
*Proceedings of the 38 th Annual Convention, Philippine<br />
Society of Animal Science, October 18-19, 2000. Manila,<br />
Philippines. p. 24-25. Also in Philippine Journal of<br />
Veterinary Animal Science, 27(1):1-11.<br />
B-25<br />
MUSCLE FIBER DIAMETER OF SELECTED<br />
MUSCLES AND SENSORY CHARACTERISTICS OF<br />
CARABEEF FROM <strong>PHILIPPINE</strong> CARABAO (Bubalis<br />
carabanensis) AND CROSSBREEDS*<br />
V. P. Obsioma, Assistant Professor, IAS-CA, UPLB<br />
S. P. Horn, Assistant Professor, IAS-CA, UPLB<br />
M. L. S. Mangabat, Undergraduate Student, IAS-CA, UPLB<br />
C. L. Tiongson, Undergraduate Student, IAS-CA, UPLB<br />
A. S. Sarabia, Supervising Science Research Specialist, PCC-DA, UPLB<br />
B. A. Oliveros, Director, PCC-DA, UPLB<br />
Two studies were conducted to compare the muscle fiber<br />
diameter of selected muscles, sensory characteristics,<br />
shear force value and firmness of carabeef from the<br />
Philippine carabao (PC) and Phil-Murrah (PM) crossbreeds<br />
(Murrah buffalo x PC or PM F 1 and PM x PM crossbreeds<br />
or PM F 2 ). Four animals from each breedtype were used<br />
in each stucy. In study I, psoas major, semitendinosus<br />
and supraspinatus muscles were taken from the left<br />
carcasses, for muscle fiber diameter determination using a<br />
compound microscope ser at low power objective (400x).<br />
In study 2, longissimus dorsi muscles from the right side of<br />
carabeef carcasses were used in penetrometer test, shear<br />
force test and sensory evaluation. Results showed no<br />
significant interaction effect of breedtypes and type of muscle<br />
on muscle fiber diameter. The mean fiber diameter of<br />
psoas major, semitendinosus and supraspinatus were<br />
31.42u, 44.83u and 59.00u, respectively, which were<br />
significantly different from each other. In study 2, loin<br />
samples from the three breedtypes were not significantly