THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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the Alb A Alb B and Alb B Alb B genotypes. The frequencies of<br />
Alb A and Alb B alleles were 0.2051 and 0.7949, respectively.<br />
Genetic equilibrium was apparent. Post-albumin<br />
polymorphism in Philippine and Malaysian carabaos was<br />
also observed. The genotypes Pa F Pa F , Pa F Pa S and Pa S<br />
Pa S were observed in both carabaos. Genotype Pa F Pa S<br />
was the most common and the frequencies of the Pa F and<br />
Pa S alleles in both carabaos were found to be similar.<br />
There appeared to be a genetic disequilibrium in both cases.<br />
Keywords: albumin and post-albumin polymorphism,<br />
Philippine carabaos, starch gel electrophoresis<br />
*M.S. Thesis, Institute of Animal Science, CA, UPLB<br />
(1972). Also in Annotated Bibliography on Philippine<br />
Biodiversity: Livestock and Poultry (Agrobiodiversity),<br />
1949-1997, Carabaos (2002) p. 248.<br />
B-21<br />
CHROMOSOMAL ANALYSIS OF <strong>WATER</strong><br />
<strong>BUFFALO</strong>ES (Bubalus bubalis) AND <strong>THE</strong>IR<br />
CROSSES IN <strong>THE</strong> <strong>PHILIPPINE</strong>S*<br />
M. A. Majid, M.S. Student, IAS-CA, UPLB<br />
Z. M. Nava, Science Research Specialist, PCRDC-PCARRD, UPLB<br />
B. A. Parker, Professor, IAS-CA, UPLB<br />
M. N. I. Faridi, International Rice Research Institute<br />
V. G. Momongan, Professor, IAS-CA, UPLB & Proj. Leader, PCRDC-<br />
PCARRD, UPLB<br />
L. A. Sitch, International Rice Research Institute<br />
Chromosome analysis of Giemsa-stained lymphocytes<br />
culture was undertaken on 5 males and 5 females in<br />
each breeding group of Murrah buffalo, Philippine carabao,<br />
F 1 hybrid (Philippine Carabao x Murrah buffalo and<br />
Philippine carabao x Nili Ravi) and F 2 animals from<br />
interse mating of F 1 offspring within each group. The<br />
karyotype of Murrah buffalo (2n=50 consisted of 10 meta<br />
or submetacentric chromosomes and 40 acrocentric<br />
including sex chromosomes. The Philippine carabao<br />
(2n=48) consisted of 10 meta or submetacentric and 38<br />
acrocentric chromosomes including two sex<br />
chromosomes (X and Y). Chromosome pair 4 in the<br />
Philippine carabao karyotype was the 4/9 tandem fusion<br />
product of Murrah karyotype. All F 1 hybrids had 2n=49<br />
chromosomes complements. A single longest metacentric<br />
chromosome was also produced by tandem fusion<br />
between one member of chromosome pair 4 and 9 which<br />
could serve as a marker to differentiate whether it is<br />
crossbred or not. The F 2 animals comprised two<br />
populations carrying 2n=48 and 2n=50 chromosomes.<br />
The “X” chromosome was the largest and the “Y”<br />
chromosome was one of the small acrocentric<br />
chromosome in the complements in all the cases.<br />
BREEDING AND GENETICS<br />
Keywords: Philippine carabao, Murrah buffalo, buffalo<br />
crossbreds, karyotype<br />
*Philippine Journal of Veterinary Animal Science,<br />
17(3&4):58 (Abstract). Also in The Philippine Agriculturist,<br />
1991. 74 (4):543-556.<br />
B-22<br />
MORPHOMETRIC AND GENETIC VARIATIONS IN<br />
<strong>THE</strong> <strong>PHILIPPINE</strong> CARABAO (Bubalus bubalis) AND<br />
TAMARAW (Anoa mindorensis Huede)*<br />
M. A. Majid, Ph.D. Student, IAS-CA, UPLB<br />
The Philippine carabao (PC) ranging from 1 to 2 years old<br />
from different locations were found significantly different in<br />
terms of morphometric characteristics such as body length<br />
(BL), height of withers (HW) and chest girth (CG).<br />
However, hip height (HH) and cannon circumference (CC)<br />
were not significantly different. In the 3 to 4 years old<br />
female animals, BL, CC, HW and TL were found<br />
significantly different while HH, CG, head length (HL) and<br />
horn length (HL) were not significantly different. The above<br />
4 yers old PCs significantly differed in body weight (BW),<br />
BL, WH, HH, CG CC, HL and TL. Sex effect was found<br />
significant only on HW. All the morphometric measurements<br />
of tamaraw were observed much lower than those of the<br />
PC. Positive correlation on all the morphometric traits was<br />
found in PC and tamaraw. Of all the parameters involved<br />
in developing the prediction equations, chest girth was the<br />
best predictor for body weight of female PCs above 4 years<br />
old while body length was best predictor to 1 to 2 years old<br />
male PCs. Four blood proteins namely, albumin, hemoglobin,<br />
two macroglobulin and transferrin; two enzymes such as<br />
malate dehydrogenase and 6-phosphogluconate<br />
dehydrogenase were examined using starch gel<br />
electrophoresis and were all found to be polymorphic.<br />
Significant differences were observed in all six loci among<br />
PCs but only transferrin and and macroglobulin loci<br />
significantly differed between PC and tanaraw. Genetic<br />
distance among different populations ranged from 0.22 to<br />
0.27. The dendrogram constructed indicates that the PC<br />
and the tamaraw are genetically related.<br />
Keywords: Philippine carabao, tamaraw, morphometric<br />
characteristics, blood proteins, dehydrogenase enzymes,<br />
genetic distance, dendrogram<br />
*Ph. D. Dissertation, Institute of Animal Science, CA,<br />
UPLB (1992).<br />
B-23<br />
EFFECTS OF STORAGE AND BREEDTYPE ON <strong>THE</strong><br />
QUALITY OF <strong>BUFFALO</strong> FROZEN SEMEN*<br />
55
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