THE PHILIPPINE WATER BUFFALO

esembles closely that of the lymphoid organs, particularly
the lymph node, and does not exhibit any structure typical
of an endocrine gland. Its cellular organization presents no
marked differences from that of the early postnatal thymuses
of the bovine and other breeds of buffaloes.
Keywords: cytoarchitectural pattern, thymus, Philippine
carabao, Bubalus bubalis, ante-natal development
*The Philippine Journal of Veterinary Medicine, 15(1&2):
1-20, 1976.
A-87
HISTOLOGICAL AND HISTOCHEMICAL STUDIES
ON THE ADRENAL MEDULLA OF THE CARABAO*
C. S. Zamora, Assistant Professor, College of Veterinary Medicine
University of the Philippines Diliman
B. U. Alegado, Student
Studies on the adrenal medulla of the carabao were
undertaken to look into its general histological structure and
to identify and locate its component epinephrine and
norepinephrine secreting cells. The histological structure of
the adrenal medulla of the carabao closely resembles that
of the ox. Although an outer medullary zone composed
chiefly of cords of columnar shaped cells and an inner
medullary zone made up mainly of aggregates of polyhedral
shaped cells were generally observed, an intermingling of
the two types of parenchymal cells was also noted in
several instances. Based on the results of the iodate reaction
and differential fixation and staining technique, the two
functional types of chromaffin cells were identified, namely:
the epinephrine secreting cells located principally in the
outer medullary zone and the norepinephrine secreting
cells distributed mainly in the inner medullary zone.
Keywords: histological study, histochemical study, adrenal
gland, carabao
*The Philippine Journal of Veterinary Medicine, 6(1&2):
145-154, 1967.
A-88
SEX DIFFERENCE IN THE MORPHOLOGY OF
NEUTROPHILS IN THE PHILIPPINE CARABAO*
C. S. Zamora, Assistant Professor, College of Veterinary Medicine
University of the Philippines Diliman
E. M. Niones, Instructor, College of Veterinary Medicine, University of the
Philippines Diliman
Blood films from 106 apparently healthy carabaos of both
sexes were examined for the presence of the sex chromatin
in neutrophils. Two forms of nuclear sex appendage were
ANATOMY
observed in the neutrophils of female carabaos: the
“drumstick” appendage, present in 1-4% of the cells, and
the “sessile” form, present in 8-11% of the cells. Such
structures were rarely seen in the neutrophils of males.
The “drumstick” appendage was occasionally seen in the
nucleus of female eosinophils. Based on this study, sexual
dimorphism in the morphology of the neutrophils can be
used as a basis for sex differentiation and as a valuable aid
in the study of intersexuality and certain sexual
abnormalities in carabaos.
Keywords: sex difference, morphology, neutrophils,
Philippine carabao
*The Philippine Journal of Veterinary Medicine, 7(1&2):
92-100, 1968.
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