THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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general the most predominant endocrine cells were the<br />
acidophils. Yellow acidophils were most abundant in Philmurrah<br />
and red acidophils in pregnant carabaos.<br />
Histochemically, five types of cells were identified in the<br />
pars distalis: chromophobes, yellow acidophils, red<br />
acidophils, beta basophils and delta basophils. The delta<br />
basophils (gonadotrophs) were more numerous than the<br />
delta cells in all groups studied. Red acidophils (lactotrophs)<br />
were the most abundant in pregnant Philippine carabaos<br />
and yellow acidophils (somatotrophs) in male Philippine<br />
carabaos and Phil-murrah.<br />
Keywords: biometry, histology, histochemistry, pituitary<br />
gland, Philippine carabao, Bubalus bubalis L., Phil-murrah<br />
crossbred<br />
*MS Thesis, College of Veterinary Medicine, University<br />
of the Philippines Los Baños, College, Laguna, 1998.<br />
Copy of the thesis is available at the library of the College<br />
of Veterinary Medicine, University of the Philippines Los<br />
Baños, College, Laguna<br />
A-66<br />
BIOMETRY AND MICROSCOPIC ANATOMY OF <strong>THE</strong><br />
HYPOPHYSIS (PITUITARY GLAND) OF <strong>THE</strong><br />
<strong>PHILIPPINE</strong> SWAMP <strong>BUFFALO</strong> (Bubalus bubalis)*<br />
A. C. Ruma, Student<br />
The study aimed to describe into the histology and biometry<br />
(weight, length, width and thickness) of the pituitary gland<br />
of the Philippine swamp buffalo. A total of twenty-five (25)<br />
pituitary glands from abattoir of Guiguinto, Bulacan were<br />
collected. The glands were measured in terms of weight<br />
(grams), length, width and thickness (cm.). Five pituitary<br />
glands (3 female and 2 males) were subjected to routine<br />
paraffin technique and haematoxylin and eosin stain and<br />
studied under light microscopy. The pituitary gland weighed<br />
2.3 g in male and 2.0 g in the female. In terms of length,<br />
width and thickness, the male was found to have 2.35 cm,<br />
1.78 cm and 1.23 cm respectively while 2.1 cm, 1.66 cm<br />
and 1.28 cm with that of the female hypophysis. No<br />
significant difference between the male and female<br />
hypophysis in terms of weight, length, width and thickness<br />
was noted. Microscopic observation revealed that the pars<br />
distalis of the pituitary gland of Philippine swamp buffalo<br />
was highly vascularized where cords or cluster of<br />
parenchymal cells were closely associated to small and<br />
big colloidal structures. This was observed to be lined by<br />
squamous to cuboidal cells scattered throughout the<br />
parenchyma. Position of the pars intermedia was cranial in<br />
location in the female which almost enclosed the<br />
26<br />
ABSTRACT OF RESEARCHES ON<br />
<strong>THE</strong> <strong>PHILIPPINE</strong> <strong>WATER</strong> <strong>BUFFALO</strong><br />
neurohypophysis whereas in the male, it extended cranioventrally.<br />
Hypophyseal cleft in the Philippine swamp buffalo<br />
was not very distinct. Its identity was marked by the large<br />
colloidal structure lined by simple squamous to stratified<br />
squamous and cuboidal cells. No cilia could be distinguished<br />
on its lining epithelium. Few but large colloidal structures<br />
were observed along with blood vessels. Neurohypophysis<br />
was located cranial to the pars distalis. Pituicytes (stellate<br />
cells) predominated which were richly supplied with blood<br />
vessels. Small round cells with small rim of cytoplasm<br />
were also present along with oval or elongated cells.<br />
Keywords: biometry, histology, pituitary gland, Philippine<br />
water buffalo<br />
*Undergraduate Thesis, College of Veterinary Science<br />
and Medicine, Central Luzon State University, Muñoz<br />
City Nueva Ecija, 1998.<br />
A-67<br />
OVARIAN STRUCTURES AND FOLLICULAR<br />
POPULATION IN <strong>WATER</strong> <strong>BUFFALO</strong>ES (Bubalus<br />
bubalis)*<br />
O.F. Smith, Ph.D. Student, IGS, CLSU<br />
F.A. Adriano, Science Research Analyst, PCRDC-PCARRD, CLSU<br />
P.G. Duran, Science Research Specialist, PCRDC-PCARRD, CLSU<br />
E.C. Atabay, Science Research Specialist, PCRDC-PCARRD, CLSU<br />
S.S. Jha, M.S. Student, IGS, CLSU<br />
T. Niwat, Ph.D. Student, IGS, CLSU<br />
P. Serra, Science Aide, PCRDC-PCARRD, CLSU<br />
L.C. Cruz, Professor, DAS-CA, CLSU and Project Leader, PCRDC-<br />
PCARRD, CLSU<br />
Ovarian samples were collected from fetal, young (5-6<br />
mo.) pubertal (2 years), adult (7-8 years) and old (12-14<br />
years) water buffaloes to characterize the ovarian structures<br />
and to determine the follicular population relative to age.<br />
Immediately after the ovaries were collected, tissue<br />
samples were fixed in 10% formol-saline solution for 24<br />
hr, dehydrated and embedded in paraffin. All tissue samples<br />
were serially sectioned at 10 u. Every 20 th section was<br />
obtained and stained with periodic Acid Schiffs-<br />
Hematoxylin. Stained sections were examined under<br />
light microscope. Numerous primary follicles were<br />
observed in the outer layer of the stroma. These were<br />
surrounded with single layer of follicular cells. The growing<br />
follicles consist of two or more layers of polygonal or<br />
cuboidal cells while there was an increased follicular cells<br />
in the secondary follicles. Changes such as the increase<br />
in size of the oocytes, appearance of the zona pellucida,<br />
cumulus oophorus and accumulation of follicular fluid in the<br />
antrum characterizes the tertiary follicles. Atresia of the<br />
antral follicles was recognized by the pulling away of the<br />
follicular epithelium from the theca interna and the presence
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