THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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methods. The diameter of the seminiferous tubules<br />
increased with age, the increase being abrupt at 10½<br />
months of age, then became more or less stabilized at 25<br />
months of age. Intratubular cells remained primodial cells<br />
in 1½ to 4½ months old animals and then these gradually<br />
differentiated to spermatogonia in 8 months old animals<br />
which could indicate the start of spermatogenesis. Lumen<br />
formation manifested its first clear-cut appearance in 15<br />
months old animals. With advance in age, the lumina also<br />
increased in size. The interstitial cells were most numerous<br />
in 1½ month old carabull testes and decreases in number<br />
as the animal grew older until 8 months of age. Regeneration<br />
of the interstitial cells occurred when the animals approached<br />
pre-pubertal stage at 10½ months of age.<br />
Keywords: carabulls, interstitial cells, post-natal<br />
development, pre-pubertal stage, seminferous tubules,<br />
testes<br />
*Abstracts of Completed Researches (1983), PCRDC-<br />
PCARRD, UPLB. Also in PCC-OED, DA Abstracts:<br />
Water Buffalo Researches in the Philippines 1981-1995<br />
(1996) p. 62. Also in Philippine Journal of Veterinary Animal<br />
Science 1984, 10 (3 & 4):40 (Abstr.). Also in Annotated<br />
Bibliography on Philippine Biodiversity: Livestock and<br />
Poultry (Agrobiodiversity) 1949-1997, Carabaos (2002) p.<br />
367.<br />
A-58<br />
HISTOLOGICAL OBSERVATION OF FOLLICULAR<br />
ATRESIA IN SWAMP <strong>BUFFALO</strong>*<br />
M. B. Ocampo, PhD Student, Hokkaido University<br />
A. T. de Asis, Student, CVM, CLSU<br />
L. C. Ocampo, M. S. Student, Hokkaido University<br />
H. Kanagawa, Professor, JFVM, Hokkaido University<br />
Swamp buffalo ovaries were collected and fixed for<br />
histological examination of follicular atresia. The follicles<br />
were classified according to size into three groups: 1.0-4.0<br />
mm (small, 4.1-9.9 mm (medium) and 10.0 mm and above<br />
(large) in diameter. Also, the size of the corpus luteum on<br />
the surface of each pair of ovary was used to estimate the<br />
stage of estrus cycle. Of 312 follicles examined, 286, 18<br />
and 8 were classified as small, medium and large follicles.<br />
Of these, 238 (83.2%), 10 (55.5%) and 8 (100%),<br />
respectively were observed to manifest signs of atresia.<br />
The number of follicles examined under follicular and luteal<br />
phase of estrus cycle were 168 (53.8%) and 144 46.2%),<br />
respectively. Of these, 120 (71.04%) and 136 (94.4%),<br />
were found to have signs of atresia. The results presented<br />
here provided the basic information for studying follicular<br />
atresia in swamp buffalo. That follicle diameter and stage<br />
of the estrus cycle are both a convenient and appropriate<br />
ANATOMY<br />
basis to use in the classification and description of the<br />
course of atresia.<br />
Keywords: follicular atresia, swamp buffalo ovaries<br />
*Buffalo Bulletin. 1994. 13:51-55. Also in PCC-OED, DA<br />
Abstracts: Water Buffalo Researches in the Philippines<br />
1981-1995 (1996), p.66. Also in Annotated Bibliography on<br />
Philippine Biodiversity: Livestock and Poultry<br />
(Agrobiodiversity) 1949-1997, Carabaos (2002) p. 356.<br />
A-59<br />
A STUDY ON SOME HISTOCHEMICAL CHARAC-<br />
TERISTICS OF <strong>THE</strong> OVARY OF SEXUALLY<br />
MATURE NON-PREGNANT <strong>PHILIPPINE</strong> CARABAO<br />
(Bubalus bubalis)*<br />
T. G. Pallasigue, Student<br />
The results of some histochemical studies on the ovaries<br />
of fifty (50) sexually mature Philippine carabao are<br />
presented in the study. During the follicular phase, the<br />
intensity of PAS reaction was persistent in the zona<br />
pellucida and Call-Exner’s bodies of the growing follicle<br />
compared with the other cellular structures of the ovary.<br />
Variations in the PAS reaction were observed in the<br />
basement membrane and liquor folliculi. These structures<br />
gave an intense PAS reaction during their developmental<br />
phase but weakens as the follicle approaches maturity.<br />
During the luteal phase, developing and regressing corpus<br />
luteum gave an intense PAS reaction than the fully<br />
developed corpus luteum. However, this phase showed<br />
less intense PAS reaction than the follicular phase. PASpositive<br />
materials were observed to be saliva resistant.<br />
Using the silver impregnation and mallory’s<br />
phosphotungstic acid hematoxylin stains, the different<br />
connective tissue elements in the ovary were identified<br />
and differentiated. In both stains used, collagen fibers<br />
were observed in the tunica albuginea, connective tissues<br />
surrounding the corpus luteum and in the stroma of the<br />
ovary. Reticular fibers were abundant in the ovarian stroma<br />
and theca interna and theca externa. Elastic fibers, which<br />
gave similar reaction as collagen were present in the walls<br />
of blood vessels.<br />
Keywords: Philippine carabao, non-pregnant,<br />
histochemical characteristics of ovaries<br />
*Undergraduate Thesis, College of Veterinary Medicine,<br />
University of the Philippines, Diliman, Quezon City, 1979.<br />
A-60<br />
HISTOLOGICAL AND HISTOCHEMICAL STUDIES<br />
OF <strong>THE</strong> INTERNAL PARATHYROID OF <strong>THE</strong> MALE<br />
23