Thirteen Philippine carabao bulls and 35 crossbred male buffaloes with ages three years and above were used in the study. Testicular biopsy was used to get tissue samples from the experimental animals. A JEOL transmission electron microscope was used to observe the ultrastructure of spermatogenetic cells. Incidence of ultrastructural alterations were analyzed by means of Fisher exact probability test. Twenty-four percent (24%) of crossbred buffaloes had spermatogenic cell alterations while a significant 40% (P
methods. The diameter of the seminiferous tubules increased with age, the increase being abrupt at 10½ months of age, then became more or less stabilized at 25 months of age. Intratubular cells remained primodial cells in 1½ to 4½ months old animals and then these gradually differentiated to spermatogonia in 8 months old animals which could indicate the start of spermatogenesis. Lumen formation manifested its first clear-cut appearance in 15 months old animals. With advance in age, the lumina also increased in size. The interstitial cells were most numerous in 1½ month old carabull testes and decreases in number as the animal grew older until 8 months of age. Regeneration of the interstitial cells occurred when the animals approached pre-pubertal stage at 10½ months of age. Keywords: carabulls, interstitial cells, post-natal development, pre-pubertal stage, seminferous tubules, testes *Abstracts of Completed Researches (1983), PCRDC- PCARRD, UPLB. Also in PCC-OED, DA Abstracts: Water Buffalo Researches in the Philippines 1981-1995 (1996) p. 62. Also in Philippine Journal of Veterinary Animal Science 1984, 10 (3 & 4):40 (Abstr.). Also in Annotated Bibliography on Philippine Biodiversity: Livestock and Poultry (Agrobiodiversity) 1949-1997, Carabaos (2002) p. 367. A-58 HISTOLOGICAL OBSERVATION OF FOLLICULAR ATRESIA IN SWAMP <strong>BUFFALO</strong>* M. B. Ocampo, PhD Student, Hokkaido University A. T. de Asis, Student, CVM, CLSU L. C. Ocampo, M. S. Student, Hokkaido University H. Kanagawa, Professor, JFVM, Hokkaido University Swamp buffalo ovaries were collected and fixed for histological examination of follicular atresia. The follicles were classified according to size into three groups: 1.0-4.0 mm (small, 4.1-9.9 mm (medium) and 10.0 mm and above (large) in diameter. Also, the size of the corpus luteum on the surface of each pair of ovary was used to estimate the stage of estrus cycle. Of 312 follicles examined, 286, 18 and 8 were classified as small, medium and large follicles. Of these, 238 (83.2%), 10 (55.5%) and 8 (100%), respectively were observed to manifest signs of atresia. The number of follicles examined under follicular and luteal phase of estrus cycle were 168 (53.8%) and 144 46.2%), respectively. Of these, 120 (71.04%) and 136 (94.4%), were found to have signs of atresia. The results presented here provided the basic information for studying follicular atresia in swamp buffalo. That follicle diameter and stage of the estrus cycle are both a convenient and appropriate ANATOMY basis to use in the classification and description of the course of atresia. Keywords: follicular atresia, swamp buffalo ovaries *Buffalo Bulletin. 1994. 13:51-55. Also in PCC-OED, DA Abstracts: Water Buffalo Researches in the Philippines 1981-1995 (1996), p.66. Also in Annotated Bibliography on Philippine Biodiversity: Livestock and Poultry (Agrobiodiversity) 1949-1997, Carabaos (2002) p. 356. A-59 A STUDY ON SOME HISTOCHEMICAL CHARAC- TERISTICS OF <strong>THE</strong> OVARY OF SEXUALLY MATURE NON-PREGNANT <strong>PHILIPPINE</strong> CARABAO (Bubalus bubalis)* T. G. Pallasigue, Student The results of some histochemical studies on the ovaries of fifty (50) sexually mature Philippine carabao are presented in the study. During the follicular phase, the intensity of PAS reaction was persistent in the zona pellucida and Call-Exner’s bodies of the growing follicle compared with the other cellular structures of the ovary. Variations in the PAS reaction were observed in the basement membrane and liquor folliculi. These structures gave an intense PAS reaction during their developmental phase but weakens as the follicle approaches maturity. During the luteal phase, developing and regressing corpus luteum gave an intense PAS reaction than the fully developed corpus luteum. However, this phase showed less intense PAS reaction than the follicular phase. PASpositive materials were observed to be saliva resistant. Using the silver impregnation and mallory’s phosphotungstic acid hematoxylin stains, the different connective tissue elements in the ovary were identified and differentiated. In both stains used, collagen fibers were observed in the tunica albuginea, connective tissues surrounding the corpus luteum and in the stroma of the ovary. Reticular fibers were abundant in the ovarian stroma and theca interna and theca externa. Elastic fibers, which gave similar reaction as collagen were present in the walls of blood vessels. Keywords: Philippine carabao, non-pregnant, histochemical characteristics of ovaries *Undergraduate Thesis, College of Veterinary Medicine, University of the Philippines, Diliman, Quezon City, 1979. A-60 HISTOLOGICAL AND HISTOCHEMICAL STUDIES OF <strong>THE</strong> INTERNAL PARATHYROID OF <strong>THE</strong> MALE 23
- Page 1 and 2: ABSTRACT OF RESEARCHES ON THE PHILI
- Page 3 and 4: USER’S GUIDE The book contains pu
- Page 5 and 6: ACKNOWLEDGMENT Grateful recognition
- Page 7 and 8: CONTENTS Inoc, J. G. and C. P. Maal
- Page 9 and 10: CONTENTS Viernes, Jr., V. D. 2004.
- Page 11 and 12: CONTENTS Harada, H., et al. Ultraso
- Page 13 and 14: CONTENTS Ocampo, M. B., et al. Clea
- Page 15 and 16: CONTENTS Aranas, T. J., et al. 1968
- Page 17 and 18: CONTENTS Romulo, B. H., et al. Dige
- Page 19 and 20: ANATOMY A-1 DISTRIBUTION AND MICROS
- Page 21 and 22: of the islet. The pancreatic- polyp
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- Page 25 and 26: A-15 THE MODIFIED HORN RING METHOD
- Page 27 and 28: undifferentiated cells. Mucigenous
- Page 29 and 30: *Abstracts of Completed Researches,
- Page 31 and 32: A-31 SCANNING ELECTRON MICROSCOPIC
- Page 33 and 34: cells” had fewer zymogen granules
- Page 35 and 36: is recommended that two years old P
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- Page 39: elated peptide (CGRP), vasoative in
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- Page 53 and 54: esembles closely that of the lympho
- Page 55 and 56: obtained for total serum protein, a
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- Page 63 and 64: months to 3 years. Erythrocyte size
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- Page 73 and 74: the Alb A Alb B and Alb B Alb B gen
- Page 75 and 76: different in trms of shear force va
- Page 77 and 78: stock farms which practiced backyar
- Page 79 and 80: Ravi was at the top. The heterosis
- Page 81 and 82: & 4):61-66. Also in Annotated Bibli
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- Page 85 and 86: Keywords: embryo, in vitro fertiliz
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INSEMINATION OF PHILIPPINE CARABAO
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It was noted that regardless of the
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the presence or absence of cysteami
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R. de Vera, Laboratory Aid, PCC-OED
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on the factors affecting embryo rec
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Expanded blastocysts did nor surviv
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Early pregnancy diagnosis was condu
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ng/ml and dropped sharply at partur
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R-67 MANAGEMENT AND CLIMATIC FACTOR
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Keywords: carabao heifers, estrous
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animal draft power was designed and
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Water Buffalo Researches in the Phi
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D-13 SOME PHYSIOLOGICAL AND BIOCHEM
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FEEDING AND NUTRITION F-1 OBSERVATI
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W. L. Johnson, Former Graduate Assi
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in a majority of the carcass compon
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F-16 FEEDING VALUE OF CORN STOVER-M
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(DDMI) of rice straw per 100 kg bod
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D. D. Lorenzo, Instructor, Departme
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Tethering, soiling, communal pastur
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C. R. Legaspino, Former Student, De
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70.97 mg percent. Age and sex seeme
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levels (8.3 mg/100 ml vs. 11.0 mg/1
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for the first 7 months. Tethering p
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L. S. Castillo, Professor, Departme
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Keywords: caraheifers, chromic oxid
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similar. Addition of 1% urea in the
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espectively. Detergent fiber analys
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V. S. Subere, Former M. S. Student,
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serovars were used namely: Leptospi
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was conducted in the abattoirs of M
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Pseudomonas sp. (2.86%), Pasteurell
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*Undergraduate thesis, College of V
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upper respiratory tract of normal c
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studies are necessary to find possi
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permanent cheek teeth, 26 permanent
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uy back operation, 11 calving inter
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caraheifers, 2, 27, 45 carcass qual