THE PHILIPPINE WATER BUFFALO

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carabaos whose ages ranged from 5-7 years old. They were acquired fresh from slaughtered animals and were fixed in 10% buffered formalin. The samples were then processed for histologic examination following the routine paraffin block technique. Tissue sections were cut, stained with H & E, PAS, Masson’s Trichrome and Weigert’s stains and examined microscopically at different magnifications. Twenty acini, intralobular and interlobular ducts from male and female samples were randomly measured using micrometer and the number of secretory cells in each acinus were counted. The nasolabial glands of the Philippine Carabao were compound tubuloacinar glands and closely resembled the salivary glands structurally. The duct system consisted of the intercalated, intralobular, interlobular, and excretory ducts. No striated ducts were observed. The glands were located in the reticular layer of the dermis. The secretory units were composed of pyramidal-shaped cells with round and densely stained nuclei. Few myoepithelial cells surrounded the acini and ducts of the gland. Significant histologic differences were observed between the nasolabial gland of the male and female carabao. The connective tissue septae were more developed in the male. The acini were larger but fewer in numbers in the male than in the female. The lobules contained larger acini (27-50 u) in the male than in the female (19-40 u). The acinus in the male consisted of 8-10 secretory cells while in the female it consisted of 5-7 secretory cells. The secretory ducts had wider lumen in males than in females. The epithelial lining of the intralobular and interlobular ducts were taller (19-31 u) in the female than in the male (11-19 u and 12-25 u, respectively). With PAS stain, the Philippine carabao nasolabial gland was observed to be a mixed gland but predominantly mucous. This finding agrees with earlier reports in water buffalo and cattle. Keywords: microscopic anatomy, nasolabial glands, Philippine carabao, Bubalus bubalis *Undergraduate thesis, College of Veterinary Medicine, University of the Philippines Los Baños, 1999. A-50 A PRELIMINARY STUDY ON THE MEASUREMENTS OF THE REPRODUCTIVE ORGANS OF PREGNANT PHILIPPINE BUFFALOES (CARABAO) (Bubalus bubalis)* J. C. Muñoz, Student The present study aimed to determine the changes in the 20 ABSTRACT OF RESEARCHES ON THE PHILIPPINE WATER BUFFALO measurements of the reproductive organs of pregnant bubaline as an aid in pregnancy examination. However, the author was constrained to use the bovine fetal measurements in determining the stages of gestation although it was recognized that the two species have different gestation lengths. This was because no studies yet have been made on the bubaline in this aspect to be used as a basis. A study was made on 50 pregnant bubaline reproductive organs at the first and second trimesters of pregnancy from animals with ages ranging from 8 to 16 years and weights from 350 to 450 kilograms. The results are presented in tables. During early pregnancy, the vagina was shorter but broader. The cervix, body and horns of the uterus were generally longer and broader than those of the non-pregnant animal. The uterine tubes were relatively shorter. The ovaries with the corpora lutea were greater in length, width, thickness and weight. Those without the corpora lutea increased in length but decreased in width, thickness and weight especially as pregnancy advanced. No ova transmigration was observed. Fiftysix percent of the total pregnancies occurred in the right horn while 44% occurred in the left. In the future, it is hoped that further studies will be made on this, keeping a closer track of the breeding histories so that the definite stages of gestation could be determined, and more accurate measurements could be taken on the changes of the reproductive organs in each gestation stage. It is also suggested that greater number of sample animals per gestation stage should be used to obtain more accurate results. Limitations of the study include lack of breeding records and previous calving histories, age and plane of nutrition of the animals. Keywords: Philippine carabao, pregnant, measurements of the reproductive organs *Undergraduate Thesis, College of Veterinary Medicine, University of the Philippines, Diliman, Quezon City, 1979. A-51 IMMUNOHISTOCHEMICAL STUDY OF THE NEUROPEPTIDES IN THE STELLATE GANGLION OF THE WATER BUFFALO* T. Nasu, Faculty of Agriculture, Miyazaki University G. D. de Ocampo, Professor, College of Veterinary Medicine, University of the Philippines Los Baños H. A. Molina, Professor, College of Veterinary Medicine, University of the Philippines Los Baños S. Tateyama, Center for Laboratory Animals, Saga Medical School M. Morimoto, Center for Laboratory Animals, Saga Medical School The localization of some neuropeptides including neuropeptide Y (NPY), substance P (SP), calcitonin gene
elated peptide (CGRP), vasoative intestinal peptide (VIP), galanin (Gal), methionine enkephalin (M-ENK), tyrosine hydroxylase (TH) immunoreactivity was studied in the stellate ganglion (SG) of water buffalo. NPY, SP, Gal, SP, CGRP, VIP and M-ENK immunoreactive nerve fibers were also seen in the SG. The localization and pattern of distribution of these peptides in the water buffalo stellate ganglion were compared with those in stellate ganglia of other mammalian species. Keywords: neuropeptide, stellate ganglion, water buffalo *Fukuoka Acta Medica, 91(5): 116-122, 2000. A-52 SOME ANATOMICO-PATHOLOGICAL OBSER- VATIONS ON THE PANCREAS OF THE PHILIPPINE CARABAOS* M. N. Novilla, Assistant Professor, College of Veterinary Medicine, University of the Philippines Diliman G. T. Baoy, Student J. A. Solis, Professor, College of Veterinary Medicine, University of the Philippines Diliman Some observations on the anatomy and pathology of the carabao pancreas at slaughter are herein presented. The gross anatomy and histology of the carabao pancreas are quite similar to those of the ox. The carabao pancreas is heavier than that of the ox, the weight varying from 300- 450 grams with a mean of 386 ± 36.8 grams. Very few gross lesions were noted at slaughter even if the other viscera had lesions. Dark coloration, edema and fibrosis were the only discernible changes found. Histopathologically, vascular congestion, edema and varying degrees of leukocytic infiltrations of pancreatic fatty tissues were noted. In a few cases, fibroplasias of the ductal and vascular systems were observed. These changes were associated with incomplete bleeding, fat necrosis and fascioliasis. Keywords: anatomico-pathological observations, pancreas, Philippine carabao * The Philippine Journal of Veterinary Medicine, 9(1&2): 51-59, 1970. A-53 ULTRASTRUCTURE OF SERTOLI AND SPERMATOGENIC CELLS OF PHILIPPINE CARABAOS AND THEIR CROSSBREDS IN RELATION TO SPERM PRODUCTION * O. M. Nuñeza. Instructor, Iligan Institute of Technology, Mindanao State University, Iligan City ANATOMY Study 1 was conducted to compare the sertoli cells and spermatogenic cell morphology of Philippine carabaos and crossbreds buffaloes as well as to determine the incidence of ultrastructural alterations in both groups. Thirteen Philippine carabao bulls and twenty five crossbreds male buffaloes were used in this study. Testicular biopsy was used to get tissue samples which were prepared for histologic evaluation using the paraffin as well as electron microscopy methods. Light and electron microscopy showed active spermatogenesis in Philippine carabao bulls. A significant high incidence of ultrastructural alterations in sertoli and spermatogenic cells was observed in crossbred buffaloes (80%) as compared to the 23% occurrence Philippine carabaos. The frequently observed alterations were pyonotic nuclei (20%), and intracytoplasmic vacuolations (32%) in spermatogonia and spermatocytes. Developing spermatids exhibited nuclear bleb (8%), disintegrated nuclear membrane (28%) and some vesiculations. Sertoli cells exhibited intravavitary structures and exaggerated infoldings of the nuclear envelop (30%), nuclear bleb (16%), and intracytoplasmic vacuolations (16%). Philippine carabaos exhibited few ultrastructural alterations which were mainly intracytoplasmic vacuolations in sertoli cells (15%) and spermatogenic cells (7.6%). Study 2 was conducted to evaluate and compare the semen characteristics of three Philippine carabao bulls, three crossbreds with normal testicular morphology and three crossbreds with ultrastuctural alterations in the testes. Weekly ejaculate obtained for a duration of one month were assessed based on semen value, initial motility, sperm concentration, percentage of live and dead spermatozoa and percent sperm abnormalities. Data were analyzed using ANOVA for split plot in completely randomized design (CRD). Crossbreds with testicular alterations were found to have inferior semen quality compared to Philippine carabaos and crossbred buffaloes without testicular alterations. Keywords: ultrastuctures, sertoli cells, spermatogenic cells, Philippine carabao, sperm production *Doctoral dissertation, Institute of Animal Science, Collage of Agriculture, University of the Philippines Los Baños, 1992. A-54 ELECTRON MICROSCOPIC INVESTIGATIONS ON THE SPERMATOGENETIC CELLS OF THE PHILIPPINE CARABAOS AND THEIR CROSSBREDS* O. M. Nuñeza, Ph.D. Student, IAS-CA, UPLB V. G. Momongan, Professor, IAS-CA, UPLB & Proj. Leader, PCRDC- PCARRD, UPLB 21
Page 1 and 2: ABSTRACT OF RESEARCHES ON THE PHILI
Page 3 and 4: USER’S GUIDE The book contains pu
Page 5 and 6: ACKNOWLEDGMENT Grateful recognition
Page 7 and 8: CONTENTS Inoc, J. G. and C. P. Maal
Page 9 and 10: CONTENTS Viernes, Jr., V. D. 2004.
Page 11 and 12: CONTENTS Harada, H., et al. Ultraso
Page 13 and 14: CONTENTS Ocampo, M. B., et al. Clea
Page 15 and 16: CONTENTS Aranas, T. J., et al. 1968
Page 17 and 18: CONTENTS Romulo, B. H., et al. Dige
Page 19 and 20: ANATOMY A-1 DISTRIBUTION AND MICROS
Page 21 and 22: of the islet. The pancreatic- polyp
Page 23 and 24: (P>0.05) from that of the Philippin
Page 25 and 26: A-15 THE MODIFIED HORN RING METHOD
Page 27 and 28: undifferentiated cells. Mucigenous
Page 29 and 30: *Abstracts of Completed Researches,
Page 31 and 32: A-31 SCANNING ELECTRON MICROSCOPIC
Page 33 and 34: cells” had fewer zymogen granules
Page 35 and 36: is recommended that two years old P
Page 37: Their secretions, together with tho
Page 41 and 42: methods. The diameter of the semini
Page 43 and 44: appearance to the fungiform papilla
Page 45 and 46: of pyknotic nuclei in the follicula
Page 47 and 48: observed. Continuous medullary patt
Page 49 and 50: examination of the muzzle prints no
Page 51 and 52: conspicuously absent in the tunica
Page 53 and 54: esembles closely that of the lympho
Page 55 and 56: obtained for total serum protein, a
Page 57 and 58: A study in the conception rate of t
Page 59 and 60: significantly (P
Page 61 and 62: and Philippine-Murrah in health. Ei
Page 63 and 64: months to 3 years. Erythrocyte size
Page 65 and 66: were highly significant level (P
Page 67 and 68: THE CATTLE AND CARABAO BREEDING PRO
Page 69 and 70: estimated breeding values of (EBVs)
Page 71 and 72: and broken horn were common among t
Page 73 and 74: the Alb A Alb B and Alb B Alb B gen
Page 75 and 76: different in trms of shear force va
Page 77 and 78: stock farms which practiced backyar
Page 79 and 80: Ravi was at the top. The heterosis
Page 81 and 82: & 4):61-66. Also in Annotated Bibli
Page 83 and 84: Series of experiment were conducted
Page 85 and 86: Keywords: embryo, in vitro fertiliz
Page 87 and 88: The percentages of pregnant cattle
Page 89 and 90:
after the second injection. The con
Page 91 and 92:
INSEMINATION OF PHILIPPINE CARABAO
Page 93 and 94:
It was noted that regardless of the
Page 95 and 96:
the presence or absence of cysteami
Page 97 and 98:
R. de Vera, Laboratory Aid, PCC-OED
Page 99 and 100:
on the factors affecting embryo rec
Page 101 and 102:
Expanded blastocysts did nor surviv
Page 103 and 104:
Early pregnancy diagnosis was condu
Page 105 and 106:
ng/ml and dropped sharply at partur
Page 107 and 108:
R-67 MANAGEMENT AND CLIMATIC FACTOR
Page 109 and 110:
Keywords: carabao heifers, estrous
Page 111 and 112:
animal draft power was designed and
Page 113 and 114:
Water Buffalo Researches in the Phi
Page 115 and 116:
D-13 SOME PHYSIOLOGICAL AND BIOCHEM
Page 117 and 118:
FEEDING AND NUTRITION F-1 OBSERVATI
Page 119 and 120:
W. L. Johnson, Former Graduate Assi
Page 121 and 122:
in a majority of the carcass compon
Page 123 and 124:
F-16 FEEDING VALUE OF CORN STOVER-M
Page 125 and 126:
(DDMI) of rice straw per 100 kg bod
Page 127 and 128:
D. D. Lorenzo, Instructor, Departme
Page 129 and 130:
Tethering, soiling, communal pastur
Page 131 and 132:
C. R. Legaspino, Former Student, De
Page 133 and 134:
70.97 mg percent. Age and sex seeme
Page 135 and 136:
levels (8.3 mg/100 ml vs. 11.0 mg/1
Page 137 and 138:
for the first 7 months. Tethering p
Page 139 and 140:
L. S. Castillo, Professor, Departme
Page 141 and 142:
Keywords: caraheifers, chromic oxid
Page 143 and 144:
similar. Addition of 1% urea in the
Page 145 and 146:
espectively. Detergent fiber analys
Page 147 and 148:
V. S. Subere, Former M. S. Student,
Page 149 and 150:
serovars were used namely: Leptospi
Page 151 and 152:
was conducted in the abattoirs of M
Page 153 and 154:
Pseudomonas sp. (2.86%), Pasteurell
Page 155 and 156:
*Undergraduate thesis, College of V
Page 157 and 158:
upper respiratory tract of normal c
Page 159 and 160:
studies are necessary to find possi
Page 161 and 162:
permanent cheek teeth, 26 permanent
Page 163 and 164:
uy back operation, 11 calving inter
Page 165 and 166:
caraheifers, 2, 27, 45 carcass qual
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THE PHILIPPINE WATER BUFFALO

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