THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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elated peptide (CGRP), vasoative intestinal peptide (VIP),<br />
galanin (Gal), methionine enkephalin (M-ENK), tyrosine<br />
hydroxylase (TH) immunoreactivity was studied in the<br />
stellate ganglion (SG) of water buffalo. NPY, SP, Gal, SP,<br />
CGRP, VIP and M-ENK immunoreactive nerve fibers<br />
were also seen in the SG. The localization and pattern of<br />
distribution of these peptides in the water buffalo stellate<br />
ganglion were compared with those in stellate ganglia of<br />
other mammalian species.<br />
Keywords: neuropeptide, stellate ganglion, water buffalo<br />
*Fukuoka Acta Medica, 91(5): 116-122, 2000.<br />
A-52<br />
SOME ANATOMICO-PATHOLOGICAL OBSER-<br />
VATIONS ON <strong>THE</strong> PANCREAS OF <strong>THE</strong> <strong>PHILIPPINE</strong><br />
CARABAOS*<br />
M. N. Novilla, Assistant Professor, College of Veterinary Medicine,<br />
University of the Philippines Diliman<br />
G. T. Baoy, Student<br />
J. A. Solis, Professor, College of Veterinary Medicine, University of the<br />
Philippines Diliman<br />
Some observations on the anatomy and pathology of the<br />
carabao pancreas at slaughter are herein presented. The<br />
gross anatomy and histology of the carabao pancreas are<br />
quite similar to those of the ox. The carabao pancreas is<br />
heavier than that of the ox, the weight varying from 300-<br />
450 grams with a mean of 386 ± 36.8 grams. Very few<br />
gross lesions were noted at slaughter even if the other<br />
viscera had lesions. Dark coloration, edema and fibrosis<br />
were the only discernible changes found. Histopathologically,<br />
vascular congestion, edema and varying<br />
degrees of leukocytic infiltrations of pancreatic fatty tissues<br />
were noted. In a few cases, fibroplasias of the ductal and<br />
vascular systems were observed. These changes were<br />
associated with incomplete bleeding, fat necrosis and<br />
fascioliasis.<br />
Keywords: anatomico-pathological observations,<br />
pancreas, Philippine carabao<br />
* The Philippine Journal of Veterinary Medicine, 9(1&2):<br />
51-59, 1970.<br />
A-53<br />
ULTRASTRUCTURE OF SERTOLI AND<br />
SPERMATOGENIC CELLS OF <strong>PHILIPPINE</strong><br />
CARABAOS AND <strong>THE</strong>IR CROSSBREDS IN<br />
RELATION TO SPERM PRODUCTION *<br />
O. M. Nuñeza. Instructor, Iligan Institute of Technology,<br />
Mindanao State University, Iligan City<br />
ANATOMY<br />
Study 1 was conducted to compare the sertoli cells and<br />
spermatogenic cell morphology of Philippine carabaos and<br />
crossbreds buffaloes as well as to determine the incidence<br />
of ultrastructural alterations in both groups. Thirteen Philippine<br />
carabao bulls and twenty five crossbreds male buffaloes<br />
were used in this study. Testicular biopsy was used to get<br />
tissue samples which were prepared for histologic<br />
evaluation using the paraffin as well as electron microscopy<br />
methods. Light and electron microscopy showed active<br />
spermatogenesis in Philippine carabao bulls. A significant<br />
high incidence of ultrastructural alterations in sertoli and<br />
spermatogenic cells was observed in crossbred buffaloes<br />
(80%) as compared to the 23% occurrence Philippine<br />
carabaos. The frequently observed alterations were pyonotic<br />
nuclei (20%), and intracytoplasmic vacuolations (32%) in<br />
spermatogonia and spermatocytes. Developing spermatids<br />
exhibited nuclear bleb (8%), disintegrated nuclear<br />
membrane (28%) and some vesiculations. Sertoli cells<br />
exhibited intravavitary structures and exaggerated<br />
infoldings of the nuclear envelop (30%), nuclear bleb (16%),<br />
and intracytoplasmic vacuolations (16%). Philippine<br />
carabaos exhibited few ultrastructural alterations which<br />
were mainly intracytoplasmic vacuolations in sertoli cells<br />
(15%) and spermatogenic cells (7.6%). Study 2 was<br />
conducted to evaluate and compare the semen<br />
characteristics of three Philippine carabao bulls, three<br />
crossbreds with normal testicular morphology and three<br />
crossbreds with ultrastuctural alterations in the testes.<br />
Weekly ejaculate obtained for a duration of one month were<br />
assessed based on semen value, initial motility, sperm<br />
concentration, percentage of live and dead spermatozoa<br />
and percent sperm abnormalities. Data were analyzed<br />
using ANOVA for split plot in completely randomized design<br />
(CRD). Crossbreds with testicular alterations were found<br />
to have inferior semen quality compared to Philippine<br />
carabaos and crossbred buffaloes without testicular<br />
alterations.<br />
Keywords: ultrastuctures, sertoli cells, spermatogenic cells,<br />
Philippine carabao, sperm production<br />
*Doctoral dissertation, Institute of Animal Science, Collage<br />
of Agriculture, University of the Philippines Los Baños,<br />
1992.<br />
A-54<br />
ELECTRON MICROSCOPIC INVESTIGATIONS ON<br />
<strong>THE</strong> SPERMATOGENETIC CELLS OF <strong>THE</strong><br />
<strong>PHILIPPINE</strong> CARABAOS AND <strong>THE</strong>IR<br />
CROSSBREDS*<br />
O. M. Nuñeza, Ph.D. Student, IAS-CA, UPLB<br />
V. G. Momongan, Professor, IAS-CA, UPLB & Proj. Leader, PCRDC-<br />
PCARRD, UPLB<br />
21