THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
THE PHILIPPINE WATER BUFFALO
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cells” had fewer zymogen granules and numeroud dilated<br />
smooth endoplasmic reticulum. Pancreatic islets were very<br />
few in number. A, B, C and D cells were identified. B cells<br />
were the most predominant cell type. Their characteristic<br />
feature was the presence of many pleomorphic granules.<br />
Majority of the granules were round containing a<br />
homogenous mass surrounded by a helo of variable size.<br />
In some cells, there were numerous granules appearing<br />
like dilated vacuole-like bodies containing pleomorphic<br />
crystalloids bodies. The secretory granules of B cells<br />
measured from 0.22µm-0.38µm. The secretory granules<br />
of A cells were more uniform in shape than B cells and<br />
consisted of dense homogenous core surrounded by a<br />
tight membrane. They appeared to be uniformly distributed<br />
around the nucleus. The granules measured about 0.13µm-<br />
0.20µm. The secretory granules of D cells appeared<br />
almost identical to those of A cells except that they were<br />
fewer in number and less electron dense. They measured<br />
approximately 0.16µm-0.22µm. C cells has scanty and<br />
pale cytoplasm containing very few secretory granules.<br />
Some C cells completely lacked secretory granules. X, F,<br />
and E cells demonstrated in other species were not<br />
observed in the Philippine water buffalo. The ultrastuctural<br />
features of A, B, C and D cells of the pancreatic islets and<br />
the pancreatic acinus of the Philippine water buffalo generally<br />
conformed with those of other species of domestic animals.<br />
Keywords: electron microscopic study, pancreatic acinus,<br />
pancreatic islets, Philippine water buffalo<br />
* The Philippine Journal of Veterinary Medicine, 41 (1): 1-<br />
9, 2004.<br />
A-37<br />
GROSS AND MICROSCOPIC CHARACTERISTICS<br />
OF <strong>THE</strong> SMALL AND LARGE LUTEAL CELLS OF<br />
CORPUS LUTEUM OF PREGNANCY OF <strong>THE</strong><br />
<strong>PHILIPPINE</strong> <strong>WATER</strong> <strong>BUFFALO</strong> (Bubalus bubalis) *<br />
C. P. Maala, Professor, College of Veterinary Medicine, University of the<br />
Philippines Los Baños<br />
M. T. S. Oñate, Assistant Professor, College of Veterinary Medicine,<br />
University of the Philippines Los Baños<br />
The corpus luteum of the Philippine water buffalo (Bubalus<br />
bubalis L) during the early, middle, and late stages of<br />
pregnancy was studied using hematoxylin and eosin<br />
stained sections. Grossly, the corpus luteum appeared<br />
pinkish red in color at the early stage of pregnancy and<br />
was almost entirely embedded in the ovary, except for the<br />
crown, which protruded into the ovarian stroma. During<br />
middle and late stages of pregnancy, the corpus luteum<br />
turned brownish in color and as pregnancy progressed, it<br />
decreased in size. Microscopically, a well-developed<br />
ANATOMY<br />
connective tissue layer composed mainly of collagen fibers<br />
surrounded the corpus luteum and divided it into lobules of<br />
varying sizes. Large and small luteal cells made up the<br />
cell population of the corpus luteum. The large cells were<br />
polygonal in shape, with a large eccentric euchromatic<br />
nucleus and eosinophilic cytoplasm. Cytoplasmic lipid<br />
droplets were observed in large cells during the early<br />
stages of pregnancy, which appeared to decrease in amount<br />
in the middle and late stages of pregnancy. Eosinophilic<br />
granules were visible in the cytoplasm of large cells in the<br />
mid stage and were most numerous in the late stage of<br />
pregnancy. Small cells were darker with a dense<br />
heterochromic nucleus. Small cells and large cells were<br />
largest during mid-pregnancy and early pregnancy,<br />
respectively (P